Characterization of Newly Developed Wheat/Barley Introgression Lines in Respect of Aluminium Tolerance

Darkó, Éva; Barnabás, Beáta; Molnár-Láng, Márta

doi:10.4236/ajps.2012.310176

Cited by 6 publications

(7 citation statements)

References 31 publications

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“…On such type of soil average concentrations of total Al varies typically between 42 mg/kg in the 0-30 cm soil layer to 21 mg/kg in the 125-170 cm layer (Altermann et al 2005). Solubility of Al in the soil arouses as a problem for plant growth at pH below 5 (Darkó et al 2012). Therefore, under the present conditions, the toxicity of Al was not an issue.…”

Section: Methodsmentioning

confidence: 79%

“…At soil solution with pH > 6, Al is present in the form of an anion Al(OH) 4 but its phytotoxic features were not studied in detail (Ma et al 2003). Since small grains are very important crops, a lot of research has been done with the aim to understand and alter mechanisms of tolerance towards high concentrations of Al and the results show that significant differences exist between different genotypes (Tang et al 2002, Darkó et al 2012.…”

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confidence: 99%

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Genetic differences in aluminium accumulation in the grains of field grown Aegilops and Triticum

Maksimović¹,

Kastori²,

Putnik-Delić³

et al. 2020

PLANT SOIL ENVIRON

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Plant species and genotypes differ considerably with respect to the accumulation of mineral elements. This study examined the accumulation of aluminium (Al) in Aegilops and Triticum species with different genomes (AA, BB, BBAA, BBAADD and DD) and correlations between concentration of Al in the grain and features of the spike. Twenty different genotypes were included in three-year field experiments. The examined species and genomes differed significantly in their Al concentration in grain. The highest concentrations of Al were found in the grains of wild diploid Aegilops speltoides (BB genome), and the lowest in tetraploids (BBAA genome). A significant positive correlation was found between the concentration of Al in the grain and spike length, while negative correlations were found between concentration of Al in the grain and the number of grains per spike, grain weight per spike and thousand grains weight. The presence of higher Al content in the individual grains of tetraploid and hexaploid wheat with respect to diploid ancestors suggests that during the increase in ploidity the capacity of plants to uptake Al from soil increased concomitantly with the increase of grain capacity to serve as Al sink.

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Section: Methodsmentioning

confidence: 79%

mentioning

confidence: 99%

Genetic differences in aluminium accumulation in the grains of field grown Aegilops and Triticum

Maksimović¹,

Kastori²,

Putnik-Delić³

et al. 2020

PLANT SOIL ENVIRON

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“…Manas is a winter barley cultivar with good winter hardiness making it better adapted to Central European climatic conditions than Betzes. It also has favourable agronomic traits, including good yielding ability [ 9 ] and better salt and aluminium tolerance than the other two barley genotypes previously used to develop addition lines [ 28 – 30 ]. Wheat-alien ditelosomic additions have also been developed using other cultivated and wild relatives of wheat, including various cultivars of rye, Aegilops species, Thinopyrum ponticum , Elymus species, Leymus racemosus and Hordeum chilense .…”

Section: Discussionmentioning

confidence: 99%

“…Introgression lines are useful for investigating heterologous expression of genes from alien chromosomes in the wheat genetic background [ 56 ]. In previous experiments several wheat/barley addition and translocation lines carrying chromosomes from different wheat and barley cultivars were studied for aluminium and salt tolerance [ 29 , 30 ]. A comparison of the salt stress responses of Asakaze/Manas wheat–barley disomic addition lines (2H, 3H, 3HS, 4H, 6H, 7H and 7HL) to those of the parental genotypes (Asakaze, Chinese Spring and Manas) revealed that the disomic addition line 7H and the ditelosomic line 7HL exhibited higher salt tolerance both during germination and in the early developmental stages than the wheat parents, among which Asakaze had higher salt tolerance than Chinese Spring [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Addition of Manas barley chromosome arms to the hexaploid wheat genome

et al. 2016

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BackgroundCultivated barley belongs to the tertiary genepool of hexaploid wheat. Genes of interest can be transferred from barley into wheat through wide hybridization. The application of wheat-barley introgression lines could provide an excellent tool for the transfer of earliness, favourable amino acid composition, biotic stress resistance, abiotic stress tolerance, or good tillering ability into wheat.ResultsA set of 10 wheat-barley ditelosomic addition lines (2HS, 2HL, 3HS, 3HL, 4HS, 4HL, 6HS, 6HL, 7HS and 7HL) was developed from the progenies of an Asakaze/Manas wheat-barley hybrid produced in Martonvásár, Hungary. The addition lines were selected from self-fertilized plants of the BC2F2-BC2F4 generations using genomic in situ hybridization (GISH) and were identified by fluorescence in situ hybridization (FISH) with repetitive DNA probes [HvT01, (GAA)7 and centromere-specific (AGGGAG)4 probes]. The cytogenetic identification was confirmed using barley arm-specific SSR and STS markers. The ditelosomic additions were propagated in the phytotron and in the field, and morphological parameters (plant height, tillering, length of the main spike, number of seeds/spike and seeds/plant, and spike characteristics) were described. In addition, the salt stress response of the ditelosomic additions was determined.ConclusionsThe six-rowed winter barley cultivar Manas is much better adapted to Central European environmental conditions than the two-rowed spring barley Betzes previously used in wheat-barley crosses. The production of wheat-barley ditelosomic addition lines has a wide range of applications both for breeding (transfer of useful genes to the recipient species) and for basic research (mapping of barley genes, genetic and evolutionary studies and heterologous expression of barley genes in the wheat background).Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-016-0393-2) contains supplementary material, which is available to authorized users.

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“…The genetic diversity of common wheat ( Triticum aestivum L.) can be extended via interspecific hybridization. One of the promising crossing partners is barley ( Hordeum vulgare L.), which has many agronomically useful traits, such as earliness [ 1 ], tolerance of biotic [ 2 , 3 ] and abiotic (Al and salt) stress [ 4 – 7 ], good tillering ability [ 8 , 9 ] and high grain protein [ 10 ] and dietary fiber content [ 11 , 12 ], which it would be desirable to transfer into wheat.…”

Section: Introductionmentioning

confidence: 99%

Molecular cytogenetic and morphological characterization of two wheat-barley translocation lines

et al. 2018

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Barley chromosome 5H, carrying important QTLs for plant adaptation and tolerance to abiotic stresses, is extremely instable in the wheat genetic background and is eliminated in the early generations of wheat-barley crosses. A spontaneous wheat-barley 5HS-7DS.7DL translocation was previously obtained among the progenies of the Mv9kr1 x Igri hybrid. The present work reports on the transfer of the 5HS-7DS.7DL translocation into a modern wheat cultivar, Mv Bodri, in order to use it in the wheat breeding program. The comparison of the hybridization bands of DNA repeats HvT01, pTa71, (GAA)n and the barley centromere-specific (AGGGAG)n in Igri barley and the 5HS-7DS.7DL translocation, together with the visualization of the barley chromatin made it possible to determine the size of the introgressed barley segment, which was approximately 74% of the whole 5HS. Of the 29 newly developed PCR markers, whose source ESTs were selected from the Genome Zipper of barley chromosome 5H, 23 were mapped in the introgressed 1–0.26 FL 5HS bin, three were located in the missing C-0.26 FL region, while three markers were specific for 5HL. The translocation breakpoint was flanked by markers Hv7502 and Hv3949. A comparison of the parental wheat cultivars and the wheat-barley introgression lines indicated that the presence of the translocation improved tillering ability in the Mv9kr1 and Mv Bodri genetic background. The similar or better yield components under high- or low-input cultivation environments, respectively, indicated that the 5HS-7DS.7DL translocation had little or no negative effect on yield components, making it a promising genotype to improve wheat genetic diversity. These results promise to accelerate functional genomic studies on barley chromosome 5H and to support pre-breeding and breeding research on wheat.

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Characterization of Newly Developed Wheat/Barley Introgression Lines in Respect of Aluminium Tolerance

Cited by 6 publications

References 31 publications

Genetic differences in aluminium accumulation in the grains of field grown Aegilops and Triticum

Genetic differences in aluminium accumulation in the grains of field grown Aegilops and Triticum

Addition of Manas barley chromosome arms to the hexaploid wheat genome

Molecular cytogenetic and morphological characterization of two wheat-barley translocation lines

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