CHARACTERIZATION OF NICOTINE ACETYLCHOLINE RECEPTOR SUBUNITS IN THE COCKROACH <i>Periplaneta americana</i> MUSHROOM BODIES REVEALS A STRONG EXPRESSION OF β1 SUBUNIT: INVOLVEMENT IN NICOTINE‐INDUCED CURRENTS

Taillebois, Emiliane; Thany, Steeve Hervé

doi:10.1002/arch.21340

Cited by 4 publications

(5 citation statements)

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“…This is consistent with data showing that using a different antibody to ours (HPA005822; Atlas Antibodies), positive neuronal staining is present in the human brain regions of the cortex, hippocampus and cerebellum (The Human Protein Atlas). Moreover, other studies conducted in adult bees (Dupuis et al, 2011) and cockroaches (Taillebois and Thany, 2016) have found the presence of the β1 subunit in Kenyon cells of mushroom bodies, which are central integration structures involved in process of learning and memory, and in human pluripotent stem cells derived from neurons (Chatzidaki et al, 2015). Yet these three latter studies used tissue lysates, thus do not provide cellular detail of expression.…”

Section: Discussionmentioning

confidence: 99%

Cellular protein and mRNA expression of β1 nicotinic acetylcholine receptor (nAChR) subunit in brain, skeletal muscle and placenta

Aishah

Hinton

Machaalani

2017

Intl J of Devlp Neuroscience

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The β1 nicotinic acetylcholine receptor (nAChR) subunit is a muscle type subunit of this family and as such, is found predominantly in muscle. Recent reports document its expression in other tissues and cell lines including adrenal glands, carcinomas, lung and brain. However, the majority of studies were of tissue lysates, thus the cellular distribution was not determined. This study aimed to determine the cellular distribution of the β1 nAChR subunit in the brain, at both the mRNA and protein levels, using non-radioactive in situ hybridization (ISH) and immunohistochemistry (IHC), respectively, and to compare it to two muscle tissue types, skeletal and placenta. Tissue was formalin fixed and paraffin embedded (all tissue types) and frozen (placenta) from humans. Additional control tissue from the piglet and mouse brain were also studied, as was mRNA for the α3 nAChR and N-methyl-d-aspartate receptor 1 (NR1) subunit. We found no β1 nAChR subunit mRNA expression in the human and piglet brain despite strong protein expression. Some signal was seen in the mouse brain but considered inconclusive given the probes designed were not of 100% homology to the mouse. In the skeletal muscle and placenta tissues, β1 nAChR subunit mRNA expression was prominent and mirrored protein expression. No α3 nAChR or NR1 mRNA was seen in the skeletal muscle, as expected, although both subunit mRNAs were present in the placenta. This study concludes that further experiments are required to conclusively state that the β1 nAChR subunit is expressed in the human, piglet and mouse brain.

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Section: Discussionmentioning

confidence: 99%

Cellular protein and mRNA expression of β1 nicotinic acetylcholine receptor (nAChR) subunit in brain, skeletal muscle and placenta

Aishah

Hinton

Machaalani

2017

Intl J of Devlp Neuroscience

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“…Whereas, the inhibition of Pameα1, Pameα2 and Pameβ1 decreased nicotine-activated currents, which correspond to the current-voltage curve of nAChR2 subtype [ 24 ]. Moreover, the specific inhibition of the cockroach Pameβ1 subunit resulted in a decrease in nicotine-induced currents, confirming that this subunit accounts for functional nAChR subtypes [ 18 ]. These data suggest that more than three neuronal nAChR subtypes were expressed in the cockroach DUM neurons [ 24 ].…”

Section: Introductionmentioning

confidence: 92%

“…In the aim of characterizing the nAChR subunits involved in the pharmacological properties of cockroach native nAChR subtypes, eight nAChR subunits (seven α and one β subunits) were cloned in the cockroach Periplaneta americana [ 18 ], while around ten subunits were found in other insect species [ 19 , 20 , 21 , 22 , 23 ]. Indeed, no Periplaneta americana α5 (Pameα5) subunit was identified in the cockroach nervous system, suggesting that the cockroach lacked this subunit.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, no Periplaneta americana α5 (Pameα5) subunit was identified in the cockroach nervous system, suggesting that the cockroach lacked this subunit. Pameα6 and Pameα7 form a cluster with drosophila Dα7 while Pameα8 is close to drosophila Dβ2, suggesting that it corresponds more with a β rather than to an α subunit [ 18 ]. The inhibition of cockroach Pameα3, Pameα8 and Pameβ1 expression by antisense oligonucleotides reduced IMI and nicotine current amplitudes, which suggests that they could be included in nAChR1 subtype.…”

Section: Introductionmentioning

confidence: 99%

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Mode of Action of Neonicotinoid Insecticides Imidacloprid and Thiacloprid to the Cockroach Pameα7 Nicotinic Acetylcholine Receptor

Cartereau

Taillebois

Questel

et al. 2021

IJMS

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The functional expression of the cockroach Pameα7 nicotinic acetylcholine receptor subunit has been previously studied, and was found to be able to form a homomeric receptor when expressed in Xenopus laevis oocytes. In this study, we found that the neonicotinoid insecticide imidacloprid is unable to activate the cockroach Pameα7 receptor, although thiacloprid induces low inward currents, suggesting that it is a partial agonist. In addition, the co-application or 5 min pretreatment with 10 µM imidacloprid increased nicotine current amplitudes, while the co-application or 5 min pretreatment with 10 µM thiacloprid decreased nicotine-evoked current amplitudes by 54% and 28%, respectively. This suggesting that these two representatives of neonicotinoid insecticides bind differently to the cockroach Pameα7 receptor. Interestingly, the docking models demonstrate that the orientation and interactions of the two insecticides in the cockroach Pameα7 nAChR binding pocket are very similar. Electrophysiological results have provided evidence to suggest that imidacloprid and thiacloprid could act as modulators of the cockroach Pameα7 receptors.

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“…To date, several cockroach cysLGIC subunit sequences have been reported. These include the nAChR subunits α1, α2, α3, α4, α6, α7, α8 and β1 from Periplaneta americana 22,24,25 . Also, fragments of Rdl from Blattella germanica were analysed in order to investigate the basis of cyclodiene resistance 26 .…”

Section: Introductionmentioning

confidence: 99%

The cys‐loop ligand‐gated ion channel gene superfamilies of the cockroaches Blattella germanica and Periplaneta americana

Jones

Goven

Froger

et al. 2021

Pest Management Science

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BACKGROUND Cockroaches are serious urban pests that can transfer disease‐causing microorganisms as well as trigger allergic reactions and asthma. They are commonly managed by pesticides that act on cys‐loop ligand‐gated ion channels (cysLGIC). To provide further information that will enhance our understanding of how insecticides act on their molecular targets in cockroaches, we used genome and reverse transcriptase polymerase chain reaction (RT‐PCR) data to characterize the cysLGIC gene superfamilies from Blattella germanica and Periplaneta americana. RESULTS The B. germanica and P. americana cysLGIC superfamilies consist of 30 and 32 subunit‐encoding genes, respectively, which are the largest insect cysLGIC superfamilies characterized to date. As with other insects, the cockroaches possess ion channels predicted to be gated by acetylcholine, γ‐aminobutyric acid, glutamate and histamine, as well as orthologues of the drosophila pH‐sensitive chloride channel (pHCl), CG8916 and CG12344. The large cysLGIC superfamilies of cockroaches are a result of an expanded number of divergent nicotinic acetylcholine receptor subunits, with B. germanica and P. americana, respectively, possessing eight and ten subunit genes. Diversity of the cockroach cysLGICs is also broadened by alternative splicing and RNA A‐to‐I editing. Unusually, both cockroach species possess a second glutamate‐gated chloride channel as well as another CG8916 subunit. CONCLUSION These findings on B. germanica and P. americana enhance our understanding of the evolution of the insect cysLGIC superfamily and provide a useful basis for the study of their function, the detection and management of insecticide resistance, and for the development of improved pesticides with greater specificity towards these major pests. © 2020 Society of Chemical Industry

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CHARACTERIZATION OF NICOTINE ACETYLCHOLINE RECEPTOR SUBUNITS IN THE COCKROACH Periplaneta americana MUSHROOM BODIES REVEALS A STRONG EXPRESSION OF β1 SUBUNIT: INVOLVEMENT IN NICOTINE‐INDUCED CURRENTS

Cited by 4 publications

References 46 publications

Cellular protein and mRNA expression of β1 nicotinic acetylcholine receptor (nAChR) subunit in brain, skeletal muscle and placenta

Cellular protein and mRNA expression of β1 nicotinic acetylcholine receptor (nAChR) subunit in brain, skeletal muscle and placenta

Mode of Action of Neonicotinoid Insecticides Imidacloprid and Thiacloprid to the Cockroach Pameα7 Nicotinic Acetylcholine Receptor

The cys‐loop ligand‐gated ion channel gene superfamilies of the cockroaches Blattella germanica and Periplaneta americana

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