Characterization of non-flocculent cells isolated from a culture of flocculent Saccharomyces cerevisiae NCYC 1001

Abstract

During cultivation of a flocculent yeast, Saccharomyces cerevisiae 1001, two cell fractions, flocs and free cells, appeared in the medium. Free cells contained cells with a normal ability to flocculate, less flocculent cells and not-flocculent cells. When the non-flocculent cells and not-flocculent cells. When the non-flocculent cell fraction from the postexponential phase of growth was collected and used as an inoculum, the culture showed synchronous growth. The floc forming ability of the yeast cells from this culture increased gradually with the number of divisions.

Flocculation onset, growth phase, and genealogical age in Saccharomyces cerevisiae

“…Since during the budding period the assembling of the newly synthesized glucan and mannoprotein is predominantly confined to the growing bud (Tkacz and Lampen 1972;Cabib et al 1982), the cell wall of the growing bud was practically de novo synthesized, keeping very little material from the parental cell wall (Cabib et al 1982). This fact raises the possibility that the zero-age daughter cells do not possess the flocculation components in their cell walls (i.e., flocculation lectins and (or) carbohydrate receptors), as it was suggested by Bielecki and Brzeski ( 1989), contributing to the dramatical loss of flocculation observed when the growth starts (Fig. 3).…”

Biologia e fusão de protoplastos de leveduras floculantes e H>sub<2>/sub<S>sup<->/sup<

“…e segundoStratford & Assinder (1991) esta formação geralmente ocorre no final da fase exponencial ou durante a fase estacionária Bielecki & Brzeski (1989),. contrapondo-se aos autores acima, relataram a ocorrência de floculação em células de Saccharomyces cerevisiae durante todos os estágios do crescimento, sugerindo que este fenômeno seja dependente da disponibilidade de sítios receptores, necessitando ainda, que os mesmos encontrem-se em correta conformação para que as células formem agregados estáveis.…”