Characterization of non-newtonian behavior during mixing of bacterial cellulose in a bioreactor

Kouda, Tohru; Yano, Hisato; Yoshinaga, Fumihiro; Kaminoyama, Meguru; Kamiwano, Mitsuo

doi:10.1016/0922-338x(96)89155-0

Cited by 59 publications

(19 citation statements)

References 12 publications

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“…In addition to this, the doubling time of A. xylinum was found increase in submerged conditions (4–6 h) compared to static condition (8–10 d) . Kouda et al studied the behavior of the culture medium during the mixing of BC in a stirred tank reactor. The rheological properties of the BC culture broth were found to be non‐Newtonian; shear‐thinning behavior was observed.…”

Section: Bioreactor Systems For Bc Productionmentioning

confidence: 99%

More Than Meets the Eye in Bacterial Cellulose: Biosynthesis, Bioprocessing, and Applications in Advanced Fiber Composites

et al. 2013

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Bacterial cellulose (BC) nanofibers are one of the stiffest organic materials produced by nature. It consists of pure cellulose without the impurities that are commonly found in plant-based cellulose. This review discusses the metabolic pathways of cellulose-producing bacteria and the genetic pathways of Acetobacter xylinum. The fermentative production of BC and the bioprocess parameters for the cultivation of bacteria are also discussed. The influence of the composition of the culture medium, pH, temperature, and oxygen content on the morphology and yield of BC are reviewed. In addition, the progress made to date on the genetic modification of bacteria to increase the yield of BC and the large-scale production of BC using various bioreactors, namely static and agitated cultures, stirred tank, airlift, aerosol, rotary, and membrane reactors, is reviewed. The challenges in commercial scale production of BC are thoroughly discussed and the efficiency of various bioreactors is compared. In terms of the application of BC, particular emphasis is placed on the utilization of BC in advanced fiber composites to manufacture the next generation truly green, sustainable and renewable hierarchical composites.

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Section: Bioreactor Systems For Bc Productionmentioning

confidence: 99%

More Than Meets the Eye in Bacterial Cellulose: Biosynthesis, Bioprocessing, and Applications in Advanced Fiber Composites

et al. 2013

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“…Agitated culture, on the other hand, is more suitable for the commercial-scale production as higher production rates can be achieved. So with the aim of improving BC production in agitated culture, the screening of suitable strains, improvement of the producing organisms, medium components and some basic operational parameters about diVerent kinds of reactors, including optimal oxygen supply, volumetric agitation power and so on, have been investigated [7,[11][12][13]. But this method has posed some problems, e.g.…”

Section: Introductionmentioning

confidence: 99%

Effect of addition of sodium alginate on bacterial cellulose production by Acetobacter xylinum

Zhou

Sun

et al. 2007

J Ind Microbiol Biotechnol

144

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Bacterial cellulose (BC) production by Acetobacter xylinum NUST4.1 was carried out in the shake flask and in a stirred-tank reactor by means of adding sodium alginate (NaAlg) into the medium. When 0.04% (w/v) NaAlg was added in the shake flask, BC production reached 6.0 g/l and the terminal yield of the cellulose was 27% of the total sugar initially added, compared with 3.7 g/l and 24% in the control, respectively. The variation between replicates in all determinations was less than 5%. During the cultivation in the stirred-tank reactor, the addition of NaAlg changed the morphology of cellulose from the irregular clumps and fibrous masses entangled in the internals to discrete masses dispersing into the broth, which indicates that NaAlg hinders formation of large clumps of BC, and enhances cellulose yield. Because the structure of cellulose is changed depending on the culture condition such as additives, structural characteristics of BC produced in the NaAlg-free and NaAlg medium are compared using scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD). SEM photographs show some differences in reticulated structures and ribbon width and FT-IR spectra indicate that there is the hydrogen bonding interaction between BC and NaAlg, then X-ray diffraction (XRD) analysis reveals that BC produced with NaAlg-added has a lower crystallinity and a smaller crystalline size. The results show that enhanced yields and modification of cellulose structure occur in the presence of NaAlg.

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“…The crystallinity index, Young's modulus, and the degree of polymerization of bacterial cellulose in fibrous form were lower than for those in pellicle form [14]. Moreover, the accumulation of bacterial cellulose in fibrous form during cultivation increased the viscosity of the broth, causing difficulty in mixing and reducing the oxygen‐transfer rate [13,15].…”

Section: Introductionmentioning

confidence: 99%

Cultivation of Acetobacter xylinum for bacterial cellulose production in a modified airlift reactor

Cheng¹,

Wang²,

Chen³

et al. 2002

Biotechnol. Appl. Biochem.

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Acetobacter xylinum for bacterial cellulose production was cultivated in a modified airlift reactor. Better results were obtained from the modified reactor than from a conventional bubble column. After 72 h of cultivation, the final concentration of bacterial cellulose was 7.72 g/l and the productivity was 0.107 g/l per h in the modified airlift reactor. The concentration of bacterial cellulose was about three times higher than that produced in the conventional bubble column. Moreover, the bacterial cellulose produced using the modified reactor formed a unique elliptical pellet (the average diameter was 10 mm), which is different from the fibrous form produced using the stirred-tank reactor. The modified airlift reactor with the suspended bacterial cellulose in pellet form had a higher volumetric oxygen-transfer coefficient and mixing capability than that with bacterial cellulose in fibrous form. The dissolved oxygen in the modified airlift reactor could be maintained above 35% throughout the cultivation.

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Characterization of non-newtonian behavior during mixing of bacterial cellulose in a bioreactor

Cited by 59 publications

References 12 publications

More Than Meets the Eye in Bacterial Cellulose: Biosynthesis, Bioprocessing, and Applications in Advanced Fiber Composites

More Than Meets the Eye in Bacterial Cellulose: Biosynthesis, Bioprocessing, and Applications in Advanced Fiber Composites

Effect of addition of sodium alginate on bacterial cellulose production by Acetobacter xylinum

Cultivation of Acetobacter xylinum for bacterial cellulose production in a modified airlift reactor

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