2008
DOI: 10.1093/pcp/pcn076
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Characterization of novel genes induced by sexual adhesion and gamete fusion and of their transcriptional regulation in Chlamydomonas reinhardtii

Abstract: When mating type plus and minus gametes of Chlamydomonas are mixed, they agglutinate with each other via their flagella, fuse, then initiate the zygote formation program which includes synthesis of the zygote cell wall, fusion of nuclei and chloroplasts, and the digestion of chloroplast DNA from the minus parent. The mRNAs from gamete and zygote cells was isolated and hybridized to cDNA-macroarray filters both to identify new genes expressed during the mating reaction and the early zygote formation process and… Show more

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Cited by 19 publications
(27 citation statements)
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“…The newly formed zygote triggers the synthesis of a new zygote extracellular matrix (ECM). Among the studied genes involved in ECM formation (Rodriguez et al 1999, Kubo et al 2008, EZY (early zygote expressed) 12, 13 and 23 are highly conserved among all algae, while others (EZY5, 6, 11, 14 and GAS28) are not. The fusion of opposite mating type gametes initiates the diploid phase of the Chlamydomonas life cycle.…”
Section: A Molecular Perspective On Fertility Genetic Controlmentioning
confidence: 99%
“…The newly formed zygote triggers the synthesis of a new zygote extracellular matrix (ECM). Among the studied genes involved in ECM formation (Rodriguez et al 1999, Kubo et al 2008, EZY (early zygote expressed) 12, 13 and 23 are highly conserved among all algae, while others (EZY5, 6, 11, 14 and GAS28) are not. The fusion of opposite mating type gametes initiates the diploid phase of the Chlamydomonas life cycle.…”
Section: A Molecular Perspective On Fertility Genetic Controlmentioning
confidence: 99%
“…Genes whose expression is known to be gamete or zygote specific, including GSM1 (minus gametes), SAG1 (plus gametes), and EZY1 (early zygotes), were retained by our filters (Armbrust et al, 1993;Kurvari et al, 1998;Ferris et al, 2002Ferris et al, , 2005Lee et al, 2008). Of the 32 previously described zygotic genes (Matters and Goodenough, 1992;Armbrust et al, 1993;Uchida et al, 1993Uchida et al, , 1999Kuriyama et al, 1999;Suzuki et al, 2000;Ferris et al, 2002;Kubo et al, 2008), 25 were in our zygote data set, while the remaining seven (EZY6, EZY15, EZY16, EZY17, EZY21, EZY23, and Lysozome 1A) were found to have significant expression in other samples and, therefore, were excluded from the zygotespecific set (Supplemental Tables S1 and S2).…”
Section: Gamete and Zygote-specific Genesmentioning
confidence: 99%
“…While previous high-throughput expression studies have focused on the transcriptional programs underlying processes such as nutrient deprivation (Nguyen et al, 2008;González-Ballester et al, 2010;Toepel et al, 2011Toepel et al, , 2013Schmollinger et al, 2014), environmental responses (Simon et al, 2008(Simon et al, , 2013Matsuo et al, 2011;Fang et al, 2012), flagellar biogenesis (Albee et al, 2013), lipid accumulation Boyle et al, 2012;Lv et al, 2013), and diurnal rhythms (Idoine et al, 2014;Panchy et al, 2014), only a few studies have explored the genome-wide transcriptional and epigenetic changes associated with the sexual cycle (Kubo et al, 2008;Ning et al, 2013;Aoyama et al, 2014). Several genes expressed in the early zygote, termed EZY genes, have predicted functions related to cell wall production, vesicular transport, and secretion (Ferris and Goodenough, 1987;Ferris et al, 2002;Kubo et al, 2008).…”
mentioning
confidence: 99%
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“…The mt+ gamete-specific protein Fus1, present on the plasma membrane of the mating structure, is essential for fusion of the mt+ and mt2 mating structures (Misamore et al, 2003). Fusion of mt+ and mt2 gametes and subsequent zygote formation entail the loss of gametic properties and the activation of the zygote developmental program, including the degradation of the Fus1 protein (Liu et al, 2010) and activation of zygote-specific genes (Armbrust et al, 1993;Uchida et al, 1993;Kuriyama et al, 1999;Uchida et al, 1999;Kubo et al, 2008). Upon fusion of mt+ and mt2 cell nuclei and chloroplasts, the zygotes develop a thick extracellular matrix and become zygospores (Woessner and Goodenough, 1989), a dormant stage in the life cycle.…”
Section: Introductionmentioning
confidence: 99%