Characterization of Novel Human Immortalized Thyroid Follicular Epithelial Cell Lines

Hopperstad, Kristen; Truschel, Theresa; Wahlicht, Tom; Stewart, Wendy; Eicher, Andrew; May, Tobias; Deisenroth, Chad

doi:10.1089/aivt.2020.0027

Cited by 7 publications

(6 citation statements)

References 40 publications

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“…[37] Thyroid follicular epithelial cells are the primary cell type of the thyroid gland and are responsible for the production and secretion of THs T4 and T3. [38] We used humanized medium (h7H) for the longterm culture of primary human follicular thyroid cells. [38,39] The optimal conditions for the culture of primary follicular thyroid epithelial cells were identified previously using h7H medium (Figure S5, Supporting Information).…”

Section: Formation Of Ts-microsphere-integrated Cellular Aggregatesmentioning

confidence: 99%

“…[38] We used humanized medium (h7H) for the longterm culture of primary human follicular thyroid cells. [38,39] The optimal conditions for the culture of primary follicular thyroid epithelial cells were identified previously using h7H medium (Figure S5, Supporting Information). Nonadhesive U-bottom plates were used for the formation of the cell aggregates (designated aggregated).…”

Section: Formation Of Ts-microsphere-integrated Cellular Aggregatesmentioning

confidence: 99%

“…[55] Recent studies have demonstrated that these cells can mimic functional thyroid tissues by accumulating iodine, producing TG, and synthesizing THs. [38] Analytical approaches for screening for toxins and hormones in biological samples are extensive and well documented. However, many rapid screening techniques often show poor sensitivity and specificity.…”

Section: Th Analysismentioning

confidence: 99%

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Thyroid‐Friendly Soft Materials as 3D Cell Culture Tool for Stimulating Thyroid Cell Function

Jun

Cho

Amos

et al. 2023

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The disruption of thyroid hormones because of chemical exposure is a significant societal problem. Chemical evaluations of environmental and human health risks are conventionally based on animal experiments. However, owing to recent breakthroughs in biotechnology, the potential toxicity of chemicals can now be evaluated using 3D cell cultures. In this study, the interactive effects of thyroid‐friendly soft (TS) microspheres on thyroid cell aggregates are elucidated and their potential as a reliable toxicity assessment tool is evaluated. Using state‐of‐the‐art characterization methods coupled with cell‐based analysis and quadrupole time‐of‐flight mass spectrometry, it is shown that TS‐microsphere‐integrated thyroid cell aggregates exhibit improved thyroid function. Specifically, the responses of zebrafish embryos, which are used for thyroid toxicity analysis, and the TS‐microsphere‐integrated cell aggregates to methimazole (MMI), a known thyroid inhibitor, are compared. The results show that the thyroid hormone disruption response of the TS‐microsphere‐integrated thyroid cell aggregates to MMI is more sensitive compared with those of the zebrafish embryos and conventionally formed cell aggregates. This proof‐of‐concept approach can be used to control cellular function in the desired direction and hence evaluate thyroid function. Thus, the proposed TS‐microsphere‐integrated cell aggregates may yield new fundamental insights for advancing in vitro cell‐based research.

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Section: Formation Of Ts-microsphere-integrated Cellular Aggregatesmentioning

confidence: 99%

Section: Formation Of Ts-microsphere-integrated Cellular Aggregatesmentioning

confidence: 99%

Section: Th Analysismentioning

confidence: 99%

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Thyroid‐Friendly Soft Materials as 3D Cell Culture Tool for Stimulating Thyroid Cell Function

Jun

Cho

Amos

et al. 2023

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“…Thereby, not only gene combinations could be identified that lead to the immortalization of the respective cell types, but also these novel established cell lines maintain the cell physiology of the primary cells they were derived from [ 137 ]. This approach was used to generate novel physiologically relevant cell systems from human and animal species such as endothelial cells [ 138 ], alveolar and airway epithelial cells [ 139 , 140 , 141 ], osteoblasts [ 142 ], mesenchymal stem cells [ 143 ], placental cells [ 144 ] and thyrocytes [ 145 ].…”

Section: Human-relevant Nams For Developmental Toxicity Testingmentioning

confidence: 99%

Human-Based New Approach Methodologies in Developmental Toxicity Testing: A Step Ahead from the State of the Art with a Feto–Placental Organ-on-Chip Platform

Luconi

Sogorb

Markert

et al. 2022

IJERPH

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Developmental toxicity testing urgently requires the implementation of human-relevant new approach methodologies (NAMs) that better recapitulate the peculiar nature of human physiology during pregnancy, especially the placenta and the maternal/fetal interface, which represent a key stage for human lifelong health. Fit-for-purpose NAMs for the placental–fetal interface are desirable to improve the biological knowledge of environmental exposure at the molecular level and to reduce the high cost, time and ethical impact of animal studies. This article reviews the state of the art on the available in vitro (placental, fetal and amniotic cell-based systems) and in silico NAMs of human relevance for developmental toxicity testing purposes; in addition, we considered available Adverse Outcome Pathways related to developmental toxicity. The OECD TG 414 for the identification and assessment of deleterious effects of prenatal exposure to chemicals on developing organisms will be discussed to delineate the regulatory context and to better debate what is missing and needed in the context of the Developmental Origins of Health and Disease hypothesis to significantly improve this sector. Starting from this analysis, the development of a novel human feto–placental organ-on-chip platform will be introduced as an innovative future alternative tool for developmental toxicity testing, considering possible implementation and validation strategies to overcome the limitation of the current animal studies and NAMs available in regulatory toxicology and in the biomedical field.

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“…Thereby, not only gene combinations could be identified that lead to the immortalization of the respective cell types but also these novel established cell lines maintain the cell physiology of the primary cells they were derived from [136]. This approach was used to generate novel physiologically relevant cell systems from human and animal species like endothelial cells [137], alveolar and airway epithelial cells [138][139][140], osteoblasts [141], mesenchymal stem cells [142], placental cells [143], and thyrocytes [144].…”

Section: Immortalization Of Cellular Models In Developmental Toxicolo...mentioning

confidence: 99%

Human-based New Approach Methodologies in Developmental Toxicity Testing: A Step Ahead from the State-of-the-art with a Feto-placental Organ-on-a-chip Platform

Luconi¹,

Sogorb²,

Markert³

et al. 2022

Preprint

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Developmental toxicity testing urgently requires the implementation of human relevant new approach methodologies (NAMs) that better recapitulate the peculiar nature of human physiology during pregnancy, especially the placenta and the maternal/fetal interface, which represent a key stage for the human lifelong health. Fit-for-purpose NAMs for the placental-fetal interface are desirable to improve the biological knowledge of environmental exposure at molecular level and to reduce the high cost, time and ethical impact of animal studies. This article reviews the state of the art on the available in vitro (placental, fetal and amniotic cell-based systems) and in silico NAMs of human relevance for developmental toxicity testing purposes, as well as of the available Adverse Outcome Pathways related to developmental toxicity. The OECD TG 414 for the identification and assessment of deleterious effects of prenatal exposure to chemicals on developing organisms will be discussed to delineate the regulatory context and to better debate what is missing and needed in the context of the developmental origins of health and disease hypothesis to significantly improve this sector. Starting from this analysis, the development of a novel human feto-placental organ-on-chip platform will be introduced as an innovative alternative tool for developmental toxicity testing, considering possible implementation and validation strategies to overcome the limitation of the current animal studies and NAMs available in regulatory toxicology and in the biomedical field.

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Characterization of Novel Human Immortalized Thyroid Follicular Epithelial Cell Lines

Abstract: Characterization of Human Thyrocyte Cell LinesFunctional Assessment in a 2D Culture Model (A) Post-expansion micrographs of huThyrEC cell lines 1-4 in huThyrEC or h7H medium. Scale bar is 100 µM.

Cited by 7 publications

References 40 publications

Thyroid‐Friendly Soft Materials as 3D Cell Culture Tool for Stimulating Thyroid Cell Function

Thyroid‐Friendly Soft Materials as 3D Cell Culture Tool for Stimulating Thyroid Cell Function

Human-Based New Approach Methodologies in Developmental Toxicity Testing: A Step Ahead from the State of the Art with a Feto–Placental Organ-on-Chip Platform

Human-based New Approach Methodologies in Developmental Toxicity Testing: A Step Ahead from the State-of-the-art with a Feto-placental Organ-on-a-chip Platform

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