Characterization of novel human ovarian cancer-specific transcripts (HOSTs) identified by serial analysis of gene expression

Rangel, Letícia Batista Azevedo; Sherman-Baust, Cheryl A.; Wernyj, Roman; Schwartz, Donald R.; Cho, Kathleen R.; Morin, Patrice J.

doi:10.1038/sj.onc.1207008

Cited by 124 publications

(87 citation statements)

References 28 publications

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“…Mutations in claudin 14 are known to cause the non-syndromic autosomal recessive deafness condition, DFNB29 (Wilcox et al 2001). Claudin 16 has been located in the TJs of the thick ascending limb of Henle, is required for Mg 2+ resorption and is over-expressed in human ovarian cancer cells (Simon et al 1999, Rangel et al 2003. Specific roles of claudins 14 and 16 in functional properties of TJs in the urothelium remain to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Characterization of tight junction proteins in cultured human urothelial cells

Rickard

Dorokhov

Ryerse

et al. 2008

In Vitro Cell.Dev.Biol.-Animal

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Tight junctions (TJs) are essential for normal function of epithelia, restricting paracellular diffusion and contributing to the maintainance of cell surface polarity. Superficial cells of the urothelium develop TJs, the basis for the paracellular permeability barrier of the bladder against diffusion of urinary solutes. Focusing on the superficial cell layer of stratified cell cultures of an immortalized human ureteral cell line, TEU-2 cells, we have examined the presence of TJ and TJ-associated proteins. TEU-2 cells were treated with calcium chloride and fetal bovine serum culture conditions used to induce stratification that resembles the normal transitional epithelial phenotype. Cultures were examined for TJ and TJ-associated proteins by confocal immuno-fluorescence microscopy and evaluated for TJ mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR). TEU-2 cultures exhibited immunoreactivity at intercellular margins for claudins 1, 4, 5, 7, 14 and 16 whereas claudins 2, 8 and 12 were intracellular. RT-PCR corroborated the presence of these claudins at the mRNA level. The TJ-associated proteins occludin, JAM-1, and zonula occludens (ZO-1, ZO-2 and ZO-3) were localized at cell margins. We have found that numerous TJs and TJ-associated proteins are expressed in stratified TEU-2 cultures. Further, we propose TEU-2s provide a useful ureteral model for future studies on the involvement of TJs proteins in the normal and pathological physiology of the human urinary system.

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Section: Discussionmentioning

confidence: 99%

Characterization of tight junction proteins in cultured human urothelial cells

Rickard

Dorokhov

Ryerse

et al. 2008

In Vitro Cell.Dev.Biol.-Animal

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“…Finally, the HOST genes are rarely expressed in normal tissues or non-ovarian cancers, but they are frequently expressed in ovarian cancer-derived cell lines and primary tumors. Therefore, the HOST genes have been proposed to be specific biomarkers and their study may lead to novel strategies for ovarian cancer diagnosis and therapy (Rangel et al, 2003). Multiple studies have addressed the ectopic expression of lncRNAs in HCC.…”

Section: Discussionmentioning

confidence: 99%

Long Non-coding RNAs are Differentially Expressed in Hepatocellular Carcinoma Cell Lines with Differing Metastatic Potential

Fang

Sun²,

Chen³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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(Talmadge and Fidler, 2010). A salient feature of metastasis is the ability of cancer cells to colonize specific organ sites (Hanahan and Weinberg, 2011). For instance, HCC cells metastasize to the lung more frequently than the lymph nodes. It is well recognized that the probability of metastatic seeding and growth is determined by both intrinsic genetic properties of the cancer cells involved and characteristics of the stromal microenvironment that surrounds them (Lu and Kang, 2007;Nguyen et al., 2009). Functional genomic analysis of HCC cell lines with different organ-specific metastatic potential may provide new opportunities for identifying diagnostic biomarkers and developing therapies to combat HCC metastasis.Large-scale genomic studies have demonstrated that the mammalian genome encodes many thousands Ting-Ting Fang et al

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“…When diagnosed at early and localized stages, the 5-year survival rate of ovarian cancer patients is approximately 94%. Nevertheless, most of the cases are detected as advanced and metastatic disease, in which cases it relapses within two years, and the patients´ survival rate decreases to 27% mainly due to chemoresistance to the platinumtaxane based chemotherapy in the adjuvant setting [57][58][59][60].…”

Section: Ovarian Cancermentioning

confidence: 99%

Metformin and mTOR Inhibitors: Allies against Ovarian and Breast Cancers

Guimarães¹,

Tessarollo²,

Santos³

et al. 2017

J Carcinog Mutagen

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Cancer is one of the leading causes of death worldwide. Every year 8.2 million people die from the disease. In this context, breast and ovarian cancer are the most incidental among women. Elucidation of cell growth pathways and the observation that these pathways are altered in human cancer have encouraged the search for specific inhibitors. The phosphatidylinositol-3cinase (PI3K)/Protein kinase b (AKT)/Mammalian Target of Rapamycin (mTOR) is an important pathway involved in cell growth, tumorigenesis, cell invasion, and resistance to therapies. This pathway is often activated in breast and ovarian cancers and the deregulation of its signaling can contribute to tumor growth, angiogenesis and metastasis. Metformin is one of the most commonly prescribed antidiabetic drugs in the world whose anticancer effects, mediated by reduced mTOR signaling, have become notable. Therefore, this review provides an overview of signaling pathway PI3K/AKT/mTOR in the ovarian and breast cancers as well as for target therapies of mTOR signaling, with an emphasis on its mechanisms, clinical applicability and future perspectives.

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Characterization of novel human ovarian cancer-specific transcripts (HOSTs) identified by serial analysis of gene expression

Cited by 124 publications

References 28 publications

Characterization of tight junction proteins in cultured human urothelial cells

Characterization of tight junction proteins in cultured human urothelial cells

Long Non-coding RNAs are Differentially Expressed in Hepatocellular Carcinoma Cell Lines with Differing Metastatic Potential

Metformin and mTOR Inhibitors: Allies against Ovarian and Breast Cancers

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