Characterization of Nucleoside Triphosphatase Activity in Isolated Pea Nuclei and Its Photoreversible Regulation by Light

Chen, Yuh-Ru; Roux, Stanley J.

doi:10.1104/pp.81.2.609

Cited by 35 publications

(19 citation statements)

References 25 publications

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Section: Methodsmentioning

confidence: 99%

“…3, the R and far-red light (FR) sources (source A) used were those described by Chen and Roux (11). Fluence rates of R were [8][9][10][11][12] jtmol/m2 sec; fluence rates for FR were 15-20 ,4mol/m2 sec.For the data in Fig. 3, R-irradiations were given in a 2-min pulse at 10-20 gmol/m2sec and measured by a quantum sensor (LI-185B; Li-cor, Lincoln, NE) by using a Schott KL1500 fiberoptic microscope illuminator (source B) filtered with a Schott RG610 filter.…”

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confidence: 99%

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Phytochrome induces changes in the immunodetectable level of a wall peroxidase that precede growth changes in maize seedlings

Kim

Shinkle

Roux

1989

Proc. Natl. Acad. Sci. U.S.A.

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The regulatory pigment phytochrome induces rapid and opposite growth changes in different regions of etiolated maize seedlings: it stimulates the elongation rate of coleoptiles and inhibits that of mesocotyls. As measured by a quantitative immunoassay, phytochrome also promotes rapid and opposite changes in the extractable content of a Mr 98,000 anionic isoperoxidase in the cell walls of these same organs: it induces a decrease of this peroxidase in coleoptiles and an increase in mesocotyls. The peroxidase changes precede the growth changes. As measured by video stereomicroscopy or a position transducer, red light (R), which photoactivates phytochrome, stimulates coleoptile elongation with a lag of about 15-20 min and suppresses mesocotyl growth with a lag of 45-50 min. R also induces a 50% reduction in the extractable level of the anionic peroxidase in coleoptile walls in less than 10 min and a 40% increase in the level of this peroxidase in mesocotyl walls within 30 min. Ascorbic acid, an inhibitor of peroxidase activity, blocks the effects of R on mesocotyl section growth.These results are relevant to hypotheses that postulate that certain wall peroxidases can participate in light-induced changes in growth rate by their effects on wall extensibility.The regulatory pigment phytochrome can initiate rapid growth changes in plants. In etiolated seedlings of oats, maize, and other grasses, the photoactivation of phytochrome by red light (R) stimulates two distinct growth responses: it promotes coleoptile elongation and inhibits mesocotyl elongation (1).Changes in the extensibility of cell walls can help to mediate certain environmentally stimulated growth changes in plants (2), including those induced by phytochrome (3). Cell wall extensibility is at least partially controlled in many plants by one or more wall-localized enzymes that catalyze the formation or breakage of cell wall bonds (4). Consistent with this finding, several authors have noted that there is an inverse correlation between wall peroxidase activity and the growth of cell walls (5); e.g., when cucumber hypocotyl elongation is inhibited by blue light, there is an increase in wall peroxidase activity (6). The inverse correlation is consistent with the function of wall peroxidases in cross-linking wall macromolecules (7). To the extent peroxidases can decrease wall extensibility, they may help to mediate the inhibitory effects Ca2+ can have on wall extensibility (8), for Ca2+ can stimulate both the activity and secretion of wall peroxidases (9).There are numerous wall isoperoxidases, and there has been little or no previously published information correlating induced growth changes with changes in the content of any specific wall peroxidase isozyme. We recently characterized a monoclonal antibody, mWP3, which specifically crossreacts with a Mr 98,000 anionic peroxidase, which represents 15% of the total peroxidases present in soluble extracts of wall proteins from maize seedlings (10). Analysis by immunogold localization methods revealed t...

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Phytochrome induces changes in the immunodetectable level of a wall peroxidase that precede growth changes in maize seedlings

Kim

Shinkle

Roux

1989

Proc. Natl. Acad. Sci. U.S.A.

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“…For example, an apyrase purified from pea nuclei was originally reported to be involved in phytochrome responses (Chen and Roux, 1986;Chen, et al, 1987). Enzymatic activity of this protein was stimulated by Ca 2ϩ /calmodulin.…”

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Differential Regulation of a Family of Apyrase Genes fromMedicago truncatula

et al. 2001

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Four putative apyrase genes were identified from the model legume Medicago truncatula. Two of the genes identified from M. truncatula (Mtapy1 and Mtapy4) are expressed in roots and are inducible within 3 h after inoculation with Sinorhizobium meliloti. The level of mRNA expression of the other two putative apyrases, Mtapy2 and Mtapy3, was unaffected by rhizobial inoculation. Screening of a bacterial artificial chromosome library of M. truncatula genomic DNA showed that Mtapy1, Mtapy3, and Mtapy4 are present on a single bacterial artificial chromosome clone. This apyrase cluster was mapped to linkage group seven. A syntenic region on soybean linkage group J was found to contain at least two apyrase genes. Screening of nodulation deficient mutants of M. truncatula revealed that two such mutants do not express apyrases to any detectable level. The data suggest a role for apyrases early in the nodulation response before the involvement of root cortical cell division leading to the nodule structure.

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“…Nuclei were gradient purified from pig liver by the same procedure used to purify pea nuclei (Chen and Roux, 1986). After the nuclei were stripped of their envelopes by treatment with EDTA and Triton X-100 (Chen et al 1987), they were treated with 0.5 M NaCl for 20 min, then centrifuged in a Fisher microcentrifuge at 13,600g for 5 min.…”

Section: Mammalian Nucleimentioning

confidence: 99%

The Major Nucleoside Triphosphatase in Pea (Pisum sativum L.) Nuclei and in Rat Liver Nuclei Share Common Epitopes Also Present in Nuclear Lamins

Tong

Dauwalder

Clawson³

et al. 1993

Plant Physiol.

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l h e major nucleoside triphosphatase (NTPase) activities in mammalian and pea (Pisum sativum 1.) nuclei are associated with enzymes that are very similar both biochemically and immunochemically. l h e major NTPase from rat liver nuclei appears to be a 46-kD enzyme that represents the N-terminal portion of lamins A and C, two lamina proteins that apparently arise from the same gene by alternate splicing. Monoclonal antibody (MAb) C2, raised to human lamin C, both immunoprecipitates the major (47 kD) NTPase in pea nuclei and recognizes i t in western blot analyses. A polyclonal antibody preparation raised to the 47-kD pea NTPase (pc480) reacts with the same lamin bands that are recognized by MAb C2 in mammalian nuclei. l h e pc480 antibodies also bind to the same lamin-like bands in pea nuclear envelope-matrix preparations that are recognized by C2 and three other MAbs known to bind to mammalian lamins. In immunofluorescence assays, pc480 and anti-lamin antibodies stain both cytoplasmic and nuclear antigens in plant cells, with slightly enhanced staining along the periphery of the nuclei. These results indicate that the pea and rat liver NTPases are structurally similar and that, in pea nuclei as in rat liver nuclei, the major NTPase i s probably derived from a lamin precursor by proteolysis.

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Characterization of Nucleoside Triphosphatase Activity in Isolated Pea Nuclei and Its Photoreversible Regulation by Light

Cited by 35 publications

References 25 publications

Phytochrome induces changes in the immunodetectable level of a wall peroxidase that precede growth changes in maize seedlings

Phytochrome induces changes in the immunodetectable level of a wall peroxidase that precede growth changes in maize seedlings

Differential Regulation of a Family of Apyrase Genes fromMedicago truncatula

The Major Nucleoside Triphosphatase in Pea (Pisum sativum L.) Nuclei and in Rat Liver Nuclei Share Common Epitopes Also Present in Nuclear Lamins

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