Characterization of Oil-Degrading Bacteria from Oil-Contaminated Soil and Activity of their Enzymes

Shaopeng, Yan; Wang, Qiuyu; Qu, Lei; Li, Cong

doi:10.5504/bbeq.2013.0050

Cited by 42 publications

(20 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The authors reported the higher ratio of C10–12 hydrocarbons and lower proportion of longer chain hydrocarbons . Furthermore, total degradation of C11–C18 and partial degradation of C19–C24 were reported by Bacillus pumilus . In the current study, Daedaleopsis sp.…”

Section: Discussionsupporting

confidence: 57%

Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.

et al. 2018

View full text Add to dashboard Cite

Petroleum, as the major energy source, is indispensable from our lives. Presence of compounds resistant to degradation can pose risks for human health and environment. Basidiomycetes have been considered as powerful candidates in biodegradation of petroleum compounds via secreting ligninolytic enzymes. In this study a wood-decaying fungus was isolated by significant degradation ability that was identified as Daedaleopsis sp. by morphological and molecular identification methods. According to GC/MS studies, incubation of heavy crude oil with Daedaleopsis sp. resulted in increased amounts of C24 compounds. Degradation of asphaltene, anthracene, and dibenzofuran by the identified fungal strain was determined to evaluate its potential in biodegradation. After 14 days of incubation, Daedaleopsis sp. could degrade 93.7% and 91.2% of anthracene and dibenzofuran, respectively, in pH 5 and 40 °C in optimized medium, as revealed by GC/FID. Notably, analysis of saturates, aromatics, resins, and asphaltenes showed a reduction of 88.7% and 38% in asphaletene and aromatic fractions. Laccase, lignin peroxidase, and manganese peroxidase activities were enhanced from 51.3, 145.2, 214.5 U ml in the absence to 121.5, 231.4, and 352.5 U ml in the presence of heavy crude oil, respectively. This is the first report that Daedaleopsis sp. can degrade asphaltene and dibenzofuran. Moreover, compared to the reported results of asphaltene biodegradation, this strain was the most successful. Thus, Daedaleopsis sp. could be a promising candidate for biotransformation of heavy crude oil and biodegradation of recalcitrant toxic compounds.

show abstract

Section: Discussionsupporting

confidence: 57%

Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.

et al. 2018

View full text Add to dashboard Cite

show abstract

“…and Pseudomonas sp. from a diesel polluted soil and [31] identified Proteus sp. as active degrader of petroleum pollutants.…”

Section: Discussionmentioning

confidence: 99%

Catechol-2,3-Dioxygenase and Crude Oil Biodegradation Screening of Rhizo-Bacterial Endophytes from Bodo-Gokana, Rivers State

Agbaji

Abu

Nwaichi

2019

CJAST

View full text Add to dashboard Cite

Strain-selection for the biotechnological application is critical in modern environmental bioremediation process design. In this study, twenty-one rhizobacterial isolates were obtained from the rhizosphere soil of Cyperus sp., Cyperus rotundus, Mariscus alternifolius and Maricus ligularis. Samples were treated using Bushnell-Haas media fortified with Bonny light crude oil plus 1% (v/v) rhizosphere soil from pre-impacted locations in Bodo-Ogoni, Gokana LGA, Rivers state. They were screened and four bacterial isolates were selected on the basis of -2,3 catechol dioxygenase activity and their growth dynamics using the growth function model in XLSTAT v 2019.1.3. Vapour-phase transfer and viable plate count techniques were employed in the determination of microbial dynamics. The order for relative enzyme activity and degradation rates followed Pseudomonas fluorescens > Achromobacter agilis > Bacillus thuringiensis > Staphylococcus lentus. The order for growth range were 7.0-10.5 Log10CFU/ml, 6.2-10.3 Log10CFU/ml, 7.1-10.1 Log10CFU/ml and 6.4-10.2 Log10CFU/ml for Achromobacter agilis > Pseudomonas fluorescens > Bacillus thuringiensis > Staphylococcus lentus. The growth pattern of these isolates fitted into the 5th order polynomial function (y= pr1+pr2*X+pr3*X2+pr4*X3+pr5*X4+pr6*X5) with R2-values of 0.999, 0.998, 0.991,0.999 compares to Gompertz and Asymptotic functions that have the least predictability with R2- values of 0.893, 0.599, 0.869, 0.894 and 0.80, 0.545, 0.829, 0.688 for the four isolates respectively. Enzyme activity of the isolates revealed that the isolates were most active on the 6th day of the study and had a lag phase within the first few hours to a day of the study. Statistical analyses revealed a significant difference using two-way ANOVA; p< 0.001 for both enzyme activity and growth rate. The results underscore the benefits and richness of rhizobacterial flora as rich in enzymatic activity for ecosystem-recovery. Overall, the study has shown the great potential and feasibility for deploying robust biotechnology for the monitoring of environmental media involving hydrocarbon pollution in the Niger Delta.

show abstract

“…According to Margesin et al (2004), soil dehydrogenase, catalase and urease activities were induced immediately after oil contamination, attaining maximum values after 11-25 days, and then declined with decreasing hydrocarbon content. Yan et al (2013) correlation between rate of crude oil degradation and dehydrogenase activity. Laccase is copper-containing oxidase enzyme which catalyzes the ring cleavage.…”

Section: Effect Of Npk and Bulking Agents On Enzyme Inductionmentioning

confidence: 99%

Induced degradation of crude oil mediated by microbial augmentation and bulking agents

Kumari

Singh

2016

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Different bacterial and fungal strains, isolated from petroleum hydrocarbon-contaminated soil, were tested, in isolation as well as in combination, for their ability to degrade total petroleum hydrocarbon (TPH) in soil samples spiked with crude oil (2, 5 or 10 %, w/w) for 30 days. The selected combination of bacterial and fungal isolates, i.e., Pseudomonas stutzeri BP10 and Aspergillus niger PS9, exhibited the highest efficiency of TPH degradation (46.7 %) in soil spiked with 2 % crude oil under control condition. Further, when this combination was applied under natural condition in soil spiked with 2 % (w/w) crude oil along with inorganic fertilizers (NPK) and different bulking agents such as rice husk, sugarcane, vermicompost or coconut coir, the percent degradation of TPH was found to be maximum (82.3 %) due to the presence of inorganic fertilizers and rice husk as bulking agent. Further, results showed that the presence of NPK and bulking agents induced the activity of degradative enzymes, such as catalase (0.718 m mol H 2 O 2 g -1 ), laccase (0.77 lmol g -1 ), dehydrogenase (37.5 lg g -1 h -1 ), catechol 1, 2 dioxygenase (276.11 l mol g -1 ) and catechol 2, 3 dioxygenase (15.15 l mol g -1 ) as compared to control (without bioaugmentation). It was inferred that the selected combination microbes along with biostimulants could accentuate the crude oil degradation as evident from the biostimulantinduced enhanced activity of degradative enzymes.

show abstract

Characterization of Oil-Degrading Bacteria from Oil-Contaminated Soil and Activity of their Enzymes

Cited by 42 publications

References 15 publications

Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.

Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.

Catechol-2,3-Dioxygenase and Crude Oil Biodegradation Screening of Rhizo-Bacterial Endophytes from Bodo-Gokana, Rivers State

Induced degradation of crude oil mediated by microbial augmentation and bulking agents

Contact Info

Product

Resources

About