Characterization of osteogenic cells grown over modified graphene-oxide-biostable polymers

Mirza, Eraj Humayun; Khan, Aftab Ahmed; Al-Khureif, Abdulaziz Abdullah; Saadaldin, Selma A.; Mohamed, Badreldin A.; Fareedi, Fatima; Khan, Muhammad Muzammil; Alfayez, Musaad; Alfotawi, Randa; Vallittu, Pekka K.; Mahmood, Amer

doi:10.1088/1748-605x/ab3ab2

Cited by 20 publications

(21 citation statements)

References 44 publications

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“…Also, as like present study graphene quantum dots (GQDs) (Dar et al, 2015) and GO-Iron oxide nanocomposites (GO-Fe 2 O 3 NCs) (Gade et al, 2015) at 50 and 100 µg/ml doses were significantly (P<0.01) decreased caprine W J-MSCs viability %. But, GO substrates did not evoke any significant cytotoxicity in murine MSCs (C3H10T1/2) (Kim et al, 2018) and in support to present study findings, Mirza et al (2019) observed that hybrid GO (GO-poly methyl methacrylate) substrates significantly increased hBM-MSCs viability.…”

Section: Cell Viabilitysupporting

confidence: 91%

In vitro Cytotoxicity Analysis of Hybrid Graphene Oxide (hGO) Nano Structures in Caprine Wharton’s Jelly Derived Mesenchymal Stem Cells (WJ-MSCs)

Dhenge

Gade

et al. 2020

IJAR

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Background: Nanotechnology is used in stem cell culture as well as in vivo delivery and tracking of stem cells. Graphene oxide (GO) is a carbon based nanomaterial and it has large surface area as well as good biocompatibility and heteroatoms doped GO exploit its properties. Hybrid GO (hGO) nano structures biocompatibility is depends on its size, dose and exposure time as well as in vitro cell models and hence, need thorough cytotoxicity studies in different species in vitro cell models. Methods: Caprine Wharton’s jelly derived mesenchymal stem cells (WJ-MSCs) were isolated, characterized and dose dependent (100, 50, 25, 10 and 0µg /ml) in vitro cytotoxicity of three different hGO nano structures (phosphorus doped graphene oxide titanium oxide tubes, rods and sheets) were analysed in caprine WJ-MSCs by studying cell cytotoxicity assays. Result: All three hGO nano structures were damaged cell morphology at 100 and 50 µg /ml doses, however, morphologically more good cells were observed in hGO tubes treated group than hGO rods and hGO sheets at 25 and 10 µg/ml doses as compared to control. Cell viability percentage was significantly (P less than 0.01) decreased at dose 100 µg/ml and it was significantly (P less than 0.01) increased at 25 µg/ml dose as compared to 50, 10 and 0 µg/ml doses. But, hGO tubes significantly (P less than 0.01) increased cell viability % as compared to hGO rods and hGO sheets. Cell population doubling time (PDT) was not altered significantly by all hGO nano structures, but 100 and 50 µg/ml doses significantly (P less than 0.01)increased cell PDT as compared to 25, 10 and 0 µg/ml doses. All hGO nano structures were non significantly altered growth curve, however, all hGO nano structures at 25 µg /ml dose altered (inclined) shape of growth curve, while 100 and 50 µg /ml doses significantly declined growth curve shape as compared to 10 and 0 µg /ml doses. Cell proliferation % was significantly (P less than 0.01) increased at 25 and 10 µg/ml doses, while, it was significantly (P less than 0.01) decreased at 100 µg /ml dose as compared to 50 and 0 µg /ml. However, there was no significance difference was observed in cell proliferation % in groups treated by different hGO nanostructures. In last, it was concluded as, hGO nano structures cytotoxicity was dose dependent and hGO nano tubes were least cytotoxic in caprine WJ-MSCs.

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Section: Cell Viabilitysupporting

confidence: 91%

In vitro Cytotoxicity Analysis of Hybrid Graphene Oxide (hGO) Nano Structures in Caprine Wharton’s Jelly Derived Mesenchymal Stem Cells (WJ-MSCs)

Dhenge

Gade

et al. 2020

IJAR

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“…According to Figure 5 , which shows the fracture zone of compression specimens of GO cement formulations, the increase in GO content generates an increase in ABC roughness that is more evident in the formulations with 0.3 and 0.5 wt.%, which can be beneficial in facilitating cell anchorage [ 16 , 18 ]. Also, some authors suggest that the surface roughness of biomaterials, such as Ti and PMMA, show a direct relationship with cell adhesion, proliferation, and differentiation [ 37 , 38 ].…”

Section: Resultsmentioning

confidence: 99%

“…Studies reported that the addition of GO to ABCs resulted in increased mechanical properties in compression [ 13 ], bending [ 14 ], fracture toughness, fatigue [ 15 ], and wear [ 16 ]; and increased biocompatibility with MC3-T3 cells [ 17 ], human bone marrow mesenchymal stem cells (hBMSCs) [ 18 ], mouse L929 fibroblasts, and human Saos-2 [ 19 ]. There are also studies about modifications to GO by silanization to improve the mechanical properties of cements [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Acrylic Bone Cements Modified with Graphene Oxide: Mechanical, Physical, and Antibacterial Properties

et al. 2020

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Bacterial infections are a common complication after total joint replacements (TJRs), the treatment of which is usually based on the application of antibiotic-loaded cements; however, owing to the increase in antibiotic-resistant microorganisms, the possibility of studying new antibacterial agents in acrylic bone cements (ABCs) is open. In this study, the antibacterial effect of formulations of ABCs loaded with graphene oxide (GO) between 0 and 0.5 wt.% was evaluated against Gram-positive bacteria: Bacillus cereus and Staphylococcus aureus, and Gram-negative ones: Salmonella enterica and Escherichia coli. It was found that the effect of GO was dependent on the concentration and type of bacteria: GO loadings ≥0.2 wt.% presented total inhibition of Gram-negative bacteria, while GO loadings ≥0.3 wt.% was necessary to achieve the same effect with Gram-positives bacteria. Additionally, the evaluation of some physical and mechanical properties showed that the presence of GO in cement formulations increased wettability by 17%, reduced maximum temperature during polymerization by 19%, increased setting time by 40%, and increased compressive and flexural mechanical properties by up to 17%, all of which are desirable behaviors in ABCs. The formulation of ABC loading with 0.3 wt.% GO showed great potential for use as a bone cement with antibacterial properties.

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“…Mirza et al [113] prepared ABCs with GO and evaluated human bone marrow mesenchymal stem cells (hBMSCs). The anabolic genes (COL1A1, BMP4, BMP2, RUNX2, and ALP) were associated with stimulatory effects, while the catabolic genes (MMP2 and MMP9) exhibited inhibitory effects in the presence of GO.…”

Section: Bioactivity In Acrylic Bone Cements Loaded With Graphene Oxidementioning

confidence: 99%

The Role of Chitosan and Graphene Oxide in Bioactive and Antibacterial Properties of Acrylic Bone Cements

2020

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Acrylic bone cements (ABC) are widely used in orthopedics for joint fixation, antibiotic release, and bone defect filling, among others. However, most commercially available ABCs exhibit a lack of bioactivity and are susceptible to infection after implantation. These disadvantages generate long-term loosening of the prosthesis, high morbidity, and prolonged and expensive treatments. Due to the great importance of acrylic bone cements in orthopedics, the scientific community has advanced several efforts to develop bioactive ABCs with antibacterial activity through several strategies, including the use of biodegradable materials such as chitosan (CS) and nanostructures such as graphene oxide (GO), with promising results. This paper reviews several studies reporting advantages in bioactivity and antibacterial properties after incorporating CS and GO in bone cements. Detailed information on the possible mechanisms by which these fillers confer bioactive and antibacterial properties to cements, resulting in formulations with great potential for use in orthopedics, are also a focus in the manuscript. To the best of our knowledge, this is the first systematic review that presents the improvement in biological properties with CS and GO addition in cements that we believe will contribute to the biomedical field.

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Characterization of osteogenic cells grown over modified graphene-oxide-biostable polymers

Cited by 20 publications

References 44 publications

In vitro Cytotoxicity Analysis of Hybrid Graphene Oxide (hGO) Nano Structures in Caprine Wharton’s Jelly Derived Mesenchymal Stem Cells (WJ-MSCs)

In vitro Cytotoxicity Analysis of Hybrid Graphene Oxide (hGO) Nano Structures in Caprine Wharton’s Jelly Derived Mesenchymal Stem Cells (WJ-MSCs)

Acrylic Bone Cements Modified with Graphene Oxide: Mechanical, Physical, and Antibacterial Properties

The Role of Chitosan and Graphene Oxide in Bioactive and Antibacterial Properties of Acrylic Bone Cements

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