Characterization of Paracoccidioides brasiliensis isolates by random amplified polymorphic DNA analysis

Soares, C. M. A.; Madlun, E E; Silva, S P da; Pereira, Maristela; Felipe, Maria Sueli Soares

doi:10.1128/jcm.33.2.505-507.1995

Cited by 56 publications

(24 citation statements)

References 18 publications

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“…This difference in DNA and protein polymorphism was previously verified in other pathogenic fungi, such as Histoplasma capsulatum 23 and P. brasiliensis. 24,25 Therefore, the results of this study, connected with previous results from our group, 22 are in accordance with other fungal models.…”

Section: Discussionsupporting

confidence: 92%

Cell‐free antigens of Sporothrix brasiliensis: antigenic diversity and application in an immunoblot assay

Almeida‐Paes¹,

Bailão²,

Pizzini³

et al. 2012

Mycoses

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Summary Sporotrichosis is a subcutaneous mycosis diagnosed by isolation of the fungus in culture. Serological tests for help in diagnosis in general do not use purified or recombinant antigens, because there is a paucity of described immunoreactive proteins, especially for the new described Sporothrix species, such as Sporothrix brasiliensis. This study aims to characterise antigens from S. brasiliensis and verify their application in serodiagnosis of sporotrichosis. An immunoblot assay allied with computer‐based analysis was used to identify putative antigenic molecules in a cell‐free extracts of both morphological phases of this fungus, and to delineate antigenic polymorphism among seven S. brasiliensis isolates and one S. schenckii Brazilian strain. The mycelial and yeast phase of the fungus originated 14 and 23 reactive bands, respectively, which were variable in intensity. An 85 kDa antigen, verified in the yeast phase of the fungus, was observed in all strains used and the immunodominant protein was identified. This protein, however, cross‐react with serum samples from patients infected with other pathogens. The results show that the S. brasiliensis cell‐free antigen extract is a single and inexpensive source of antigens, and can be applied on the sporotrichosis serodiagnosis.

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Section: Discussionsupporting

confidence: 92%

Cell‐free antigens of Sporothrix brasiliensis: antigenic diversity and application in an immunoblot assay

Almeida‐Paes¹,

Bailão²,

Pizzini³

et al. 2012

Mycoses

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“…Genetic polymorphism in P. brasiliensis has been detected in the past by RAPD analysis (6,32,37) and chromosome analyses (8,21). It is also known that individual isolates show different degrees of pathogenicity in susceptible inbred mice (15,33,35).…”

Section: Discussionmentioning

confidence: 99%

Polymorphism in the Gene Coding for the Immunodominant Antigen gp43 from the Pathogenic Fungus Paracoccidioides brasiliensis

et al. 2000

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The gp43 glycoprotein is an immune-dominant antigen in patients with paracoccidioidomycosis (PCM). It is protective against murine PCM and is a putative virulence factor. The gp43 gene of Paracoccidioides brasiliensis B-339 is located in a 1,329-bp DNA fragment that includes two exons, a 78-bp intron, and a leader peptide-coding region of 105 bp. Polymorphism in gp43 has been suggested by the occurrence, in the same isolate or among different fungal samples, of isoforms with distinct isoelectric points. In the present study we aligned and compared with a consensus sequence the gp43 precursor genes of 17 P. brasiliensis isolates after sequencing two PCR products from each fungal sample. The genotypic types detected showed 1 to 4 or 14 to 15 informative substitution sites, preferentially localized between 578 and 1166 bp. Some nucleotide differences within individual isolates (noninformative sites) resulted in a second isoelectric point for the deduced protein.The most polymorphic sequences were also phylogenetically distant from the others and encoded basic gp43 isoforms. The three isolates in this group were from patients with chronic PCM, and their DNA restriction patterns were distinct in Southern blots. The nucleotides encoding the inner core of the murine T-cell-protective epitope of gp43 were conserved, offering hope for the development of a universal vaccine.

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“…However, the interaction of A. castellanii with T16B1 revealed that this strain was more efficient in surviving and killing the amoebae. Given that the armadillo strain was isolated about 7 years ago whereas Pb18 and Pb01 were isolated about 90 and 30 years ago, respectively [40, 41], these results point to the attenuation of Paracoccidioides spp. after prolonged in vitro subculturing.…”

Section: Discussionmentioning

confidence: 99%

A hidden battle in the dirt: soil amoebae interactions withParacoccidioidesspp

Albuquerque

Nicola

Magnabosco

et al. 2019

Preprint

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26Paracoccidioides spp. are thermodimorphic pathogenic fungi endemic to Latin America. 27 Predation is believed to drive the evolution of virulence for soil saprophytes. We evaluated the 28 presence of environmental amoeboid predators in soil from armadillo burrows where 29Paracoccidioides had been previously detected and tested if interaction of Paracoccidioides with 30 amoebae increased fungal virulence. Nematodes, ciliates and amoebaeall potential predators of 31 fungigrew in cultures from soil samples. Microscopical observation and ITS sequencing 32 identified the amoebae as Acanthamoeba spp, Allovahlkampfia spelaea and Vermamoeba 33 vermiformis. These three amoebae efficiently ingested, killed and digested Paracoccidioides spp. 34 yeast cells, as did laboratory-adapted axenic Acanthamoeba castellanii. Sequential co-cultivation 35of Paracoccidioides with A. castellanii selected for phenotypical traits related to survival of the 36 fungus within a natural predator as well as in murine macrophages and in vivo (Galleria 37 mellonella and mice). This increase in virulence is linked to the accumulation of cell wall alpha-38 glucans, polysaccharides that masks recognition of fungal molecular patterns by host pattern 39 recognition receptors. Altogether, our results indicate that Paracoccidioides inhabits a complex 40 environment with multiple amoeboid predators that can exert selective pressure to guide the 41 evolution of virulence traits. 42 43

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Characterization of Paracoccidioides brasiliensis isolates by random amplified polymorphic DNA analysis

Cited by 56 publications

References 18 publications

Cell‐free antigens of Sporothrix brasiliensis: antigenic diversity and application in an immunoblot assay

Cell‐free antigens of Sporothrix brasiliensis: antigenic diversity and application in an immunoblot assay

Polymorphism in the Gene Coding for the Immunodominant Antigen gp43 from the Pathogenic Fungus Paracoccidioides brasiliensis

A hidden battle in the dirt: soil amoebae interactions withParacoccidioidesspp

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