Characterization of Pasteurellaceae-like bacteria isolated from clinically affected psittacine birds

Abstract

Aims: The aim of the present investigation was to identify and characterize Pasteurella‐like isolates obtained from clinically affected psittacine birds. Methods and Results: A total of 37 isolates from psittacine birds tentatively classified with the family Pasteurellaceae were characterized phenotypically. The genetic relationship was investigated by sequencing of partial rpoB and 16S rRNA genes for selected isolates. The results obtained were compared with the data from 16 reference strains. Nine isolates were identified as Gallibacterium spp., 16 as Volucribacter spp. or Volucribacter‐like, while 11 isolates were classified as taxon 44 of Bisgaard. A single isolate was identified as Pasteurella multocida. Conclusions: Characterization of Pasteurellaceae by traditional methods is often inconclusive because of inconsistent reactions and phenotypic diversity. For the same reason, genotyping is essential to allow proper classification as demonstrated in the present study. Significance and Impact of the Study: Limited information exists on the isolation and significance of Pasteurellaceae associated with clinically affected psittacine birds showing signs of digestive and/or respiratory disorders. The present investigations demonstrated that these organisms are widely distributed among clinically affected birds, but isolation of these taxa cannot be unambiguously correlated with the symptoms observed.

Bacterial community composition in Adélie (Pygoscelis adeliae) and Chinstrap (Pygoscelis antarctica) Penguin stomach contents from Signy Island, South Orkney Islands

“…Excepting Chelonobacter, these bacteria have been reported as common inhabitants in the guts across a variety of bird species (Bjerrum et al 2006;Strong et al 2013;Grond et al 2014;Roggenbuck et al 2014;Kreisinger et al 2015), including Antarctic penguins (Thouzeau et al 2003a;Banks et al 2009;Dewar et al 2014), however, the majority of their role in the guts remain unclear. Chelonobacter, a new bacterial genus belonging to the family Pasteurellaceae, was first discovered from diseased tortoises (Gregersen et al 2009), and has been found in human stomachs (Delgado et al 2013) but so far has not been reported in penguin or other avian gut samples. As for Clostridium (family: Clostridiaceae), some species strains have been identified to have ability to degrade chitin (Chen et al 2002), which is a main component of crustaceans including Antarctic krill (Clarke 1980;Nicol and Hosie 1993).…”

Gut Microbiome of the Critically Endangered New Zealand Parrot, the Kakapo (Strigops habroptilus)

“…4) are frequently associated with respiratory disease in psittacine birds. Although not all bacterial species in these clades are causative agents of disease, their presence should be considered a warning, as they are often found in sick birds [80]. During the 2011 breeding season several chicks were removed from the nest due to respiratory problems, although this did not cause long-term health issues in the birds (D. Eason, personal communication).…”

Prevalence, isolation and antimicrobial susceptibility of Gallibacterium anatis from Local Breed of Female Muscovy Ducks (Cairina moschata) in Maiduguri, Northeastern Nigeria

“…It has also been isolated from apparently healthy domesticated fowl (Gallus domestica) in Nigeria by Addo and Mohan (1985). The disease had been frequently isolated from diseased and apparently healthy layers and cockerels of exotic breeds of chickens as well as a wide range of semi-domestic birds including turkeys, geese, ducks, pheasants, partridges and cattle egrets (Bisgaard, 1993;Christensen et al, 2003;Rzewuska et al, 2007;Bisgaard et al, 2009;Gregersen et al, 2010; 2014; Sorour et al, 2015). The varying unhygienic environments (Plate 1 and 2) of rearing Muscovy ducks in the study area may possibly expose this species of birds to various infectious bacterial diseases including G. anatis.…”

“…Gallibacterium anatis mostly affects intensively farmed poultry birds causing loss in production with heavy mortality in broiler chicken and drop in egg production in layers with increased mortality (Bojesen et al, 2008). Gallibacterium anatis has also been reported to infect turkeys, geese, ducks, pheasants, partridges, budgerigars, peacock, cage birds, wild birds, cattle and pig (Christensen et al, 2003;Rzewuska et al, 2007;Bisgaard et al, 2009;Gregersen et al, 2010). The bacterium has been reported to be associated with fatal bacteremia in immune-compromised human patient (Aubin et al, 2013).…”