Characterization of peptide immobilization on an acetylene terminated surface via click chemistry

Shamsi, Fahimeh; Coster, H.G.L.; Jolliffe, Katrina A.

doi:10.1016/j.susc.2011.05.027

Cited by 25 publications

(20 citation statements)

References 82 publications

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“…We speculate that a low coverage of diyne 1 molecules after a short 2 or 10 min reaction suffices to reduce the dielectric screening of the carbonaceous film (Supplementary Fig. 12 ) 36 , thereby allowing for inversion, but on the other hand the poor passivation leads to the growth of SiO 2 which masks the inversion by reducing the electron transfer kinetics ( vide infra ). It is in fact very plausible that the manifestation of peak potential inversion is masked to some degree by kinetic factors, which forces a progressive peak-to-peak separation ( E p,a > E p,c ).…”

Section: Resultsmentioning

confidence: 99%

Reproducible flaws unveil electrostatic aspects of semiconductor electrochemistry

et al. 2017

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Predicting or manipulating charge-transfer at semiconductor interfaces, from molecular electronics to energy conversion, relies on knowledge generated from a kinetic analysis of the electrode process, as provided by cyclic voltammetry. Scientists and engineers encountering non-ideal shapes and positions in voltammograms are inclined to reject these as flaws. Here we show that non-idealities of redox probes confined at silicon electrodes, namely full width at half maximum <90.6 mV and anti-thermodynamic inverted peak positions, can be reproduced and are not flawed data. These are the manifestation of electrostatic interactions between dynamic molecular charges and the semiconductor’s space-charge barrier. We highlight the interplay between dynamic charges and semiconductor by developing a model to decouple effects on barrier from changes to activities of surface-bound molecules. These findings have immediate general implications for a correct kinetic analysis of charge-transfer at semiconductors as well as aiding the study of electrostatics on chemical reactivity.

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Section: Resultsmentioning

confidence: 99%

Reproducible flaws unveil electrostatic aspects of semiconductor electrochemistry

et al. 2017

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“…The binding energies observed were consistent with the results reported elsewhere 24,43,51 . The absence of a Si-O peak in the 101-104 eV region of the high-resolution Si 2p scan indicated good quality monolayers that can effectively protect the underlying silicon substrate from oxidation 45,47,52 (Figure 1c). The alkyne-terminated organic monolayers on non-oxidized silicon surfaces provide a chemical handle for further functionalization via CuAAC "click" chemistry.…”

Section: Resultsmentioning

confidence: 99%

“Click” Patterning of Self-Assembled Monolayers on Hydrogen-Terminated Silicon Surfaces and Their Characterization Using Light-Addressable Potentiometric Sensors

Wang

Watkinson

et al. 2015

Langmuir

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Two potential strategies for chemically patterning alkyne-terminated self-assembled monolayers (SAMs) on oxide-free silicon or silicon-on-sapphire (SOS) substrates were investigated and compared. The patterned surfaces were validated using a light-addressable potentiometric sensor (LAPS) for the first time. The first strategy involved an integration of photolithography with "click" chemistry. Detailed surface characterization (i.e. water contact angle, ellipsometry, AFM, and XPS) and LAPS measurements showed that photoresist processing not only decreases the coverage of organic monolayers but also introduces chemically bonded contaminants on the surfaces, thus significantly reducing the quality of the SAMs and the utility of "click" surface modification. The formation of chemical contaminants in photolithography was also observed on carboxylic acid- and alkyl-terminated monolayers using LAPS. In contrast, a second approach combined microcontact printing (μCP) with "click" chemistry; that is azide (azido-oligo(ethylene glycol) (OEG)-NH2) inks were printed on alkyne-terminated SAMs on silicon or SOS through PDMS stamps. The surface characterization results for the sample printed with a flat featureless PDMS stamp demonstrated a nondestructive and efficient method of μCP to perform "click" reactions on alkyne-terminated, oxide-free silicon surfaces for the first time. For the sample printed with a featured PDMS stamp, LAPS imaging showed a good agreement with the pattern of the PDMS stamp, indicating the successful chemical patterning on non-oxidized silicon and SOS substrates and the capability of LAPS to image the molecular patterns with high sensitivity.

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“…They are attributed respectively to the vibration of the primary amine NH 2 and N—H scissoring bending. This proves that protein molecules are well attached on the functionalized Si surface (Mark et al , ; Hiraoui et al , ; Shamsi et al , ).…”

Section: Resultsmentioning

confidence: 76%

“…Usually, oxidation of the Si surface is demonstrated by IR spectroscopy in the low‐frequency regions. In the range from 600 to 1400 cm −1 is shown the most informative features of particular relevance, one can observe the presence of a Si―O―Si link at 1015 cm −1 (Hiraoui et al , ; Shamsi et al , ) proving that the Si surface was oxidized. In this work, we demonstrate these characteristics and modifications due to AA plasma treatment through AES, as described in the succeeding text.…”

Section: Resultsmentioning

confidence: 99%

Direct immobilization of avidin protein on AFM tip functionalized by acrylic acid vapor at RF plasma

Costa

Vilani

Peripolli

et al. 2012

J of Molecular Recognition

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The atomic force microscopy (AFM) has been used as a force sensor to measure unbinding forces of single bound complexes in the nanonewton and piconewton range. Force spectroscopy measurements can be applied to study both intermolecular and intramolecular interactions of complex biological and synthetic macromolecules. Although the AFM has been extensively used as a nano force sensor, the commercially available cantilever is limited to silicon and silicon nitride. Those materials reduce the adhesion sensitivity with specific surface and/or molecule. Here, we functionalized the AFM tip with carboxylic groups by applying acrylic acid (AA) vapor at radio frequency plasma treatment at 100 W for 5 min. This method provides a remarkable sensitivity enhancement on the functional group interaction specificity. The functionalized tip was characterized by scanning electron microscopy. The electron beam high resolution images have not shown significant tip sharpness modification. Silicon wafers (1 0 0)-no treated and functionalized by AA plasma treatment-were characterized by Auger electron spectroscopy to elucidate the silicon surface sputtering and demonstrate functionalization. The Fourier transform-infrared spectroscopy spectrum shows a high absorbance of avidin protein over the silicon surface functionalized by AA plasma treatment.We carried out force spectroscopy assay to measure the unbinding force between the well-established pair biotin-avidin. At pulling speed of 2 µm/s, we measured the unbinding force of 106 ± 23 pN, which is in good agreement with the literature, demonstrating the effectiveness of the tip functionalization by AA plasma treatment in biological studies.

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Characterization of peptide immobilization on an acetylene terminated surface via click chemistry

Cited by 25 publications

References 82 publications

Reproducible flaws unveil electrostatic aspects of semiconductor electrochemistry

Reproducible flaws unveil electrostatic aspects of semiconductor electrochemistry

“Click” Patterning of Self-Assembled Monolayers on Hydrogen-Terminated Silicon Surfaces and Their Characterization Using Light-Addressable Potentiometric Sensors

Direct immobilization of avidin protein on AFM tip functionalized by acrylic acid vapor at RF plasma

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