Characterization of perfusion decellularized whole animal body, isolated organs, and multi‐organ systems for tissue engineering applications

Taylor, Doris A.; Kren, Stefan M.; Rhett, Katrina; Robertson, Matthew J.; Morrissey, Jacquelynn; Rodriguez, Osman E.; Virk, Hassan; Chacon‐Alberty, Lourdes; Costa, Ernesto Curty da; Mesquita, Fernanda; Sampaio, Luiz C.; Hochman‐Mendez, Camila

doi:10.14814/phy2.14817

Cited by 16 publications

(4 citation statements)

References 25 publications

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“…Interestingly, Taylor and colleagues in their perfusion decellularization model of a whole animal body and multi‐organ systems reported on the mechanical properties of decellularized heart‐lung ECM block (24). Their results demonstrated Ultimate tensile strength and Young's modulus of the heart to be significantly higher than those of the lung, explaining the results in terms of the organs' collagen content and fiber formation.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of a novel combined perfusion decellularization heart‐lung model for tissue engineering of bioartificial heart‐lung scaffolds

et al. 2022

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Combined heart-lung transplantation remains the only definitive treatment for end-stage pulmonary and cardiac disease. Nevertheless, the rates of this life-saving procedure have been constantly decreasing, mainly owing to the restrictive indications, such as severe pulmonary hypertension with right ventricular failure, congenital heart disease, idiopathic interstitial pneumonia, as well as the severe donor-organ shortage that transplant surgery is facing. The International Thoracic Organ Transplant Registry only reports 59 heart-lung transplantations performed in 2017 (1). Furthermore, the long-term complications and side effects of immunosuppressive therapy, including hypertension, diabetes mellitus, malignancy, and chronic allograft rejection remain a common concern (2). Tissue engineering represents a promising concept for organ and tissue replacement and may constitute a novel approach to

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Section: Discussionmentioning

confidence: 99%

Development and evaluation of a novel combined perfusion decellularization heart‐lung model for tissue engineering of bioartificial heart‐lung scaffolds

et al. 2022

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“…Although not the main focus of this review, it is worth noting that these decellularization techniques have also been explored in whole organs context, by using decellularization by perfusion, which leads to the conservation of the organ-derived 3D native ECM with an intact vascular tree [ 117 ]. This offers a route for the scaffold recellularization with site-specific cells and/or stem, and progenitor cells [ 118 ].…”

Section: Isolation and Decellularization Of Ecmmentioning

confidence: 99%

Extracellular matrix-derived materials for tissue engineering and regenerative medicine: A journey from isolation to characterization and application

Vilaça-Faria,

Noro,

Reis

et al. 2024

Bioactive Materials

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“…Detergent perfusion involves accurate and stable cannulation of the chosen vessel to allow efficient solution diffusion throughout the organ [45]. Ovarian blood circulation is simple, consisting of one major artery and a vein.…”

Section: Whole-ovary Perfusion Decellularizationmentioning

confidence: 99%

Perfusion and Ultrasonication Produce a Decellularized Porcine Whole-Ovary Scaffold with a Preserved Microarchitecture

Almeida,

da Silva-Júnior,

Gibin

et al. 2023

Cells

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The application of decellularized scaffolds for artificial tissue reconstruction has been an approach with great therapeutic potential in regenerative medicine. Recently, biomimetic ovarian tissue reconstruction was proposed to reestablish ovarian endocrine functions. Despite many decellularization methods proposed, there is no established protocol for whole ovaries by detergent perfusion that is able to preserve tissue macro and microstructure with higher efficiency. This generated biomaterial may have the potential to be applied for other purposes beyond reproduction and be translated to other areas in the tissue engineering field. Therefore, this study aimed to establish and standardize a protocol for porcine ovaries’ decellularization based on detergent perfusion and ultrasonication to obtain functional whole-ovary scaffolds. For that, porcine ovaries (n = 5) were perfused with detergents (0.5% SDS and 1% Triton X-100) and submitted to an ultrasonication bath to produce acellular scaffolds. The decellularization efficiency was evaluated by DAPI staining and total genomic DNA quantification. ECM morphological evaluation was performed by histological, immunohistochemistry, and ultrastructural analyses. ECM physico-chemical composition was evaluated using FTIR and Raman spectroscopy. A cytocompatibility and cell adhesion assay using murine fibroblasts was performed. Results showed that the proposed method was able to remove cellular components efficiently. There was no significant ECM component loss in relation to native tissue, and the scaffolds were cytocompatible and allowed cell attachment. In conclusion, the proposed decellularization protocol produced whole-ovaries scaffolds with preserved ECM composition and great potential for application in tissue engineering.

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Characterization of perfusion decellularized whole animal body, isolated organs, and multi‐organ systems for tissue engineering applications

Cited by 16 publications

References 25 publications

Development and evaluation of a novel combined perfusion decellularization heart‐lung model for tissue engineering of bioartificial heart‐lung scaffolds

Development and evaluation of a novel combined perfusion decellularization heart‐lung model for tissue engineering of bioartificial heart‐lung scaffolds

Extracellular matrix-derived materials for tissue engineering and regenerative medicine: A journey from isolation to characterization and application

Perfusion and Ultrasonication Produce a Decellularized Porcine Whole-Ovary Scaffold with a Preserved Microarchitecture

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