Characterization of peripheral blood T lymphocyte subsets in Chinese rhesus macaques with repeated or long-term infection with Plasmodium cynomolgi

Li, Qinyan; Ruan, Zhiyan; Zhang, Haixiang; Peng, Nanzheng; Shu, Zhao; Li, Qin; Chen, Xiaoping

doi:10.1007/s00436-011-2581-3

Cited by 7 publications

(7 citation statements)

References 35 publications

(34 reference statements)

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“…to study infectious diseases including simian immunodeficiency virus/ human immunodeficiency virus, avian influenza, malaria, plague, and severe acute respiratory syndrome, as well as studies of xenotransplantation, stem cell therapies, reproduction, and neurobiology (Shen et al 2007;Chen et al 2009Chen et al , 2011Chen et al , 2012Choi et al 2011;Liu et al 2011;Tian et al 2011;Wei et al 2011;Hutnick et al 2012;Li et al 2012b;Ling et al 2013;Mumbauer et al 2013). Yet comparatively little is known about the immunogenetics of these animals.…”

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confidence: 99%

Major Histocompatibility Complex Class I Haplotype Diversity in Chinese Rhesus Macaques

Karl

Bohn

Wiseman

et al. 2013

G3 Genes|Genomes|Genetics

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The use of Chinese-origin rhesus macaques (Macaca mulatta) for infectious disease immunity research is increasing despite the relative lack of major histocompatibility complex (MHC) class I immunogenetics information available for this population. We determined transcript-based MHC class I haplotypes for 385 Chinese rhesus macaques from five different experimental cohorts, providing a concise representation of the full complement of MHC class I major alleles expressed by each animal. In total, 123 Mamu-A and Mamu-B haplotypes were defined in the full Chinese rhesus macaque cohort. We then performed an analysis of haplotype frequencies across the experimental cohorts of Chinese rhesus macaques, as well as a comparison against a group of 96 Indian rhesus macaques. Notably, 35 of the 51 Mamu-A and Mamu-B haplotypes observed in Indian rhesus macaques were also detected in the Chinese population, with 85% of the 385 Chinese-origin rhesus macaques expressing at least one of these class I haplotypes. This unexpected conservation of Indian rhesus macaque MHC class I haplotypes in the Chinese rhesus macaque population suggests that immunologic insights originally gleaned from studies using Indian rhesus macaques may be more applicable to Chinese rhesus macaques than previously appreciated and may provide an opportunity for studies of CD8+ T-cell responses between populations. It may also be possible to extend these studies across multiple species of macaques, as we found evidence of shared ancestral haplotypes between Chinese rhesus and Mauritian cynomolgus macaques.

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confidence: 99%

Major Histocompatibility Complex Class I Haplotype Diversity in Chinese Rhesus Macaques

Karl

Bohn

Wiseman

et al. 2013

G3 Genes|Genomes|Genetics

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“…Our previous study using the rhesus monkey model indicated that Pc infection activated CD4+ T cells in SIV-negative animals [ 7 ]. In the present study, we examined activation markers of CD4+ T cells in SIV-positive animals under ART, including HLA-DR and CD38 co-expression on CD4+ T cells and plasma cytokine concentrations.…”

Section: Resultsmentioning

confidence: 99%

Plasmodium infection reduces the volume of the viral reservoir in SIV-infected rhesus macaques receiving antiretroviral therapy

et al. 2014

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BackgroundPrevious studies indicated that Plasmodium infection activates the immune system, including memory CD4+ T cells, which constitute the reservoir of human immunodeficiency virus type-1 (HIV-1). Therefore, we postulated that co-infection with malaria might activate the reservoir of HIV-1. To test this hypothesis, we used a rhesus macaque model of co-infection with malaria and simian immunodeficiency virus (SIV), along with antiretroviral therapy (ART).ResultsOur results showed that Plasmodium infection reduced both the replication-competent virus pool in resting CD4+ T cells and the integrated virus DNA (iDNA) load in peripheral blood mononuclear cells in the monkeys. This reduction might be attributable to malaria-mediated activation and apoptotic induction of memory CD4+ T cells. Further studies indicated that histone acetylation and NF-kappaB (NF-κB) activation in resting CD4+ T cells may also play an important role in this reduction.ConclusionsThe findings of this work expand our knowledge of the interaction between these two diseases. As more HIV-1-infected individuals in malaria-endemic areas receive ART, we should explore whether any of the patients co-infected with Plasmodium experience virologic benefits.Electronic supplementary materialThe online version of this article (doi:10.1186/s12977-014-0112-x) contains supplementary material, which is available to authorized users.

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“…Due to the limited supply of well-characterized Indian rhesus macaques, scientists have begun using Chinese rhesus macaques to study infectious disease such as HIV/SIV, influenza, and malaria, in addition to xenotransplantation and stem cell therapies (Southwick and Siddiqui 1994; Chen et al 2011; Chen et al 2009; Choi et al 2011; Hutnick et al 2012; Li et al 2012; Ling et al 2013; Mumbauer et al 2013; Wei et al 2011). However, characterization of MHC class I sequences in the Chinese rhesus macaque population remains incomplete.…”

Section: Introductionmentioning

confidence: 99%

Full-length novel MHC class I allele discovery by next-generation sequencing: two platforms are better than one

et al. 2013

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Deep sequencing has revolutionized major histocompatibility complex (MHC) class I analysis of nonhuman primates by enabling high-throughput, economical, and comprehensive genotyping. Full-length MHC class I cDNA sequences, which are required to generate reagents such as MHC:peptide tetramers, cannot be directly obtained by short read deep sequencing. We combined data from two next-generation sequencing platforms to discover novel full-length MHC class I mRNA/cDNA transcripts in Chinese rhesus macaques. We first genotyped macaques by Roche/454 pyrosequencing using a 530 bp amplicon spanning the densely polymorphic exons 2 through 4 of the MHC class I loci that encode the peptide-binding region. We then mapped short paired-end 250 bp Illumina sequence reads spanning the full-length transcript to each 530 bp amplicon at high stringency and used paired-end information to reconstruct full-length allele sequences. We characterized 65 full-length sequences from 6 Chinese rhesus macaques. Overall, approximately 70% of the alleles distinguished in these 6 animals contained new sequence information, including 29 novel transcripts. The flexibility of this approach should make full-length MHC class I allele genotyping accessible for any nonhuman primate population of interest. We are currently optimizing this method for full-length characterization of other highly polymorphic, duplicated loci such as the MHC class II DRB and killer immunoglobulin-like receptors. We anticipate that this method will facilitate rapid expansion and near completion of sequence libraries of polymorphic loci, such as MHC class I, within a few years.

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Characterization of peripheral blood T lymphocyte subsets in Chinese rhesus macaques with repeated or long-term infection with Plasmodium cynomolgi

Cited by 7 publications

References 35 publications

Major Histocompatibility Complex Class I Haplotype Diversity in Chinese Rhesus Macaques

Major Histocompatibility Complex Class I Haplotype Diversity in Chinese Rhesus Macaques

Plasmodium infection reduces the volume of the viral reservoir in SIV-infected rhesus macaques receiving antiretroviral therapy

Full-length novel MHC class I allele discovery by next-generation sequencing: two platforms are better than one

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