Characterization of permeability and network structure of interfacially photopolymerized poly(ethylene glycol) diacrylate hydrogels

Cruise, Gregory M.; Scharp, David S.; Hubbell, Jeffrey A.

doi:10.1016/s0142-9612(98)00025-8

Cited by 566 publications

(554 citation statements)

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“…In this report, increasing the PEG concentration decreased the swelling ratio, which is consistent with previous observations of PEG hydrogels [33]. Increasing the polymer concentration would influence both the number of crosslinks and may cause increased entanglement, leading to a decreased swelling ratio [33,34]. However an increased swelling, which corresponds to a decreased cross-link density of the hydrogel, was observed with an increasing HA content.…”

Section: Discussionsupporting

confidence: 91%

“…Addition of viscoelastic HA, which has a MW of 10 3 kDa, significantly enhances the swelling ratio of hydrogels composed primarily of PEG (MW=10 kDa) [21]. In this report, increasing the PEG concentration decreased the swelling ratio, which is consistent with previous observations of PEG hydrogels [33]. Increasing the polymer concentration would influence both the number of crosslinks and may cause increased entanglement, leading to a decreased swelling ratio [33,34].…”

Section: Discussionsupporting

confidence: 88%

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Non-viral vector delivery from PEG-hyaluronic acid hydrogels

Wieland

Houchin-Ray

Shea

2007

Journal of Controlled Release

121

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Hydrogels have been widely used in tissue engineering as a support for tissue formation or to deliver non-viral gene therapy vectors locally. Hydrogels that combine these functionalities can provide a fundamental tool to promote specific cellular processes leading to tissue formation. This report investigates controlled release of gene therapy vectors from hydrogels as a function of the physical properties for both the hydrogel and the vector. Hydrogels were formed by photocrosslinking acrylmodified hyaluronic acid (HA) with a 4-arm poly(ethylene glycol) (PEG) acryl. The polymer content, and relative composition of HA and PEG modulated the swelling ratio, water content, and degradation, which can influence transport of the vector through the hydrogel. All hydrogels had a water content of 94% or higher, though the water content and swelling ratio increased with a decrease in the PEG:HA ratio. Plasmids were stably incorporated into the hydrogel, with a majority of the release occurring during the initial 2 days. For incubation in buffer, the cumulative release increased with a decreasing PEG or increasing HA content, with approximately 20% to 80% released during the first week depending on the hydrogel composition. Hydrogels incubated in hyaluronidase, an enzyme that degrades HA, significantly increased plasmid release for hydrogels containing 4% PEG and 4% HA-Acryl. The encapsulation of plasmid complexed with polyethylenimine had less than 14% of the complexes released from the hydrogel both in the presence and absence of hyaluronidase. The limited release of the complexes likely results from the complex size and interactions between the vector and hydrogel. These studies demonstrate the dependence of non-viral vector release on the physical properties of the hydrogel and the vector, suggesting vector and hydrogel designs for maximizing localized delivery of non-viral vectors.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 88%

Non-viral vector delivery from PEG-hyaluronic acid hydrogels

Wieland

Houchin-Ray

Shea

2007

Journal of Controlled Release

121

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“…Figure 3.2 depicts the EWC data in graphical form. We also compared the water content we used against several other papers [51,52,53,54,11] that had some of the cross-linking densities we considered, and found our numbers to be reasonable.…”

Section: Solvation Of Pegda Networkmentioning

confidence: 87%

“…Deeper insight into dynamic processes occurring within hydrogels have become possible by techniques such as high-flux neutron sources and X-ray synchrotrons [6,7,8]. Diffusion in hydrogel has been studied extensively using Quasi-Elastic Neutron Scattering (QENS) [9,10], Nuclear Magnetic Resonance (NMR) [11,12,13], side-by-side diffusion cells [14], fluorescence correlation spectroscopy [15,16], refractive index method [17], etc.…”

Section: Review Of Experimental and Theoretical Studies Of Hydrogelsmentioning

confidence: 99%

Effect of Cross-Linking on the Diffusion of Water, Ions, and Small Molecules in Hydrogels

2009

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Understanding the diffusion of small molecules in hydrogel system is of major importance in a variety of applications including drug delivery systems, tissue engineering and contact lens. Cross-linking density of hydrogels has been commonly used to tune key parameters like mesh size and molecular weight between cross-linkers, in order to change macroscopic properties of hydrogels. In this thesis, molecular dynamics investigations of chemically-cross-linked poly(ethylene glycol) (PEG) hydrogels are reported with the aim of exploring the diffusion properties of water, ions, and rhodamine within the polymer at the molecular level.The water structure and diffusion properties were studied at various cross-linking densities with molecular weights of the chains ranging from 572 to 3400. As the cross-linking density is increased, the water diffusion decreases and the slowdown in diffusion is more severe at the polymer-water interface. The water diffusion at various cross-linking densities is correlated with the water hydrogen bonding dynamics. The diffusion of ions and rhodamine also decreased as the cross-linking density is increased. The variation of diffusion coefficient with cross-linking density is related to the variation of water content at different cross-linking densities.Comparison of simulation results and obstruction scaling theory for hydrogels showed similar trends.ii To Father and Mother.iii

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“…PEG is a biocompatible polymer used extensively in biomedical applications. It is soluble in aqueous solution and can be modified with photoreactive end groups to form macromonomers that, upon exposure to UV light, form crosslinked hydrogels with high equilibrium water content (30,82). PAA is an anionic polyelectrolyte that has been extensively investigated and has found wide use in biomedical applications as well as in "super-absorbent" infant diapers due to its impressive swelling properties.…”

Section: Interpenetrating Polymer Networkmentioning

confidence: 99%

Development of Hydrogel‐Based Keratoprostheses: A Materials Perspective

Myung

Duhamel

Cochran

et al. 2008

Biotechnology Progress

105

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Research and development of artificial corneas (keratoprostheses) in recent years have evolved from the use of rigid hydrophobic materials such as plastics and rubbers to hydrophilic, waterswollen hydrogels engineered to support not only peripheral tissue integration but also glucose diffusion and surface epithelialization. The advent of the AlphaCor core-and-skirt hydrogel keratoprosthesis has paved the way for a host of new approaches based on hydrogels and other soft materials that encompass a variety of materials preparation strategies, from synthetic homopolymers and copolymers to collagen-based bio-copolymers and, finally, interpenetrating polymer networks. Each approach represents a unique strategy toward the same goal: to develop a new hydrogel that mimics the important properties of natural donor corneas. We provide a critical review of these approaches from a materials perspective and discuss recent experimental results. While formidable technical hurdles still need to be overcome, the rapid progress that has been made by investigators with these approaches is indicative that a synthetic donor cornea capable of surface epithelialization is now closer to becoming a clinical reality.

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Characterization of permeability and network structure of interfacially photopolymerized poly(ethylene glycol) diacrylate hydrogels

Cited by 566 publications

References 0 publications

Non-viral vector delivery from PEG-hyaluronic acid hydrogels

Non-viral vector delivery from PEG-hyaluronic acid hydrogels

Effect of Cross-Linking on the Diffusion of Water, Ions, and Small Molecules in Hydrogels

Development of Hydrogel‐Based Keratoprostheses: A Materials Perspective

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