Characterization of peroxidase enzyme and detoxification of phenols using peroxidase enzyme obtained from Zea mays L waste

Abstract

Phenol is one of the greatest menaces among the industrial pollutants. A treatment method which utilizes agricultural wastes in a simple manner has become the need of the hour. In this investigation, details on the extraction, optimization of parameters and study of detoxification potential of the peroxidase enzyme obtained from baby corn waste have been elaborated. The enzyme has been extracted from both corn silk and husk, and studies have been conducted on both the samples. Estimation of amount of protein and enzyme activity has shown promising results, and the conditions optimized are easily attainable at larger scales, making this work feasible for scale-up.

Pathogenesis-Related Proteins (PRs) with Enzyme Activity Activating Plant Defense Responses

“…Thus, the use of plant peroxidases arouses great industrial interest as potential biodegradable agents. PRX from plants can degrade residual phenols in water, from industrial wastewater [77][78][79].…”

Synthesis of Fe3O4/Fe2O3 nanoparticles modified with peroxidase with incorporated chromium and manganese ions

“…The enzyme exhibits redox activity in the catalysis of peroxide bonds in nature. 25 It can be easily immobilized for biomedicine applications for detection of cholesterol 26 or glucose 27 and also in biocatalysis such as detoxification of phenols, 28 remediation of textile waters, 23 and reduction of azo dyes. 29 Implementation of peroxidase immobilized with iron-containing nano-sized matrixes leads to increased enzyme loading by enlarged functional surface and may result in higher stability or catalytic properties.…”

Extraction of Oxidative Enzymes from Green Tea Leaves and Optimization of Extraction Conditions

“…It should be pointed out that living organisms like birds, fungi, insects, mammals, and various kinds of plants contain PPO, which can be extracted for different applications. In plants, it is localized in association with the chloroplast thylakoid membrane or it is available in free form in the cytosol [9,10]. In terms of POD, its activity has been found in animals, plants, and microorganisms, where the different POD varieties play significant roles.…”