Characterization of PGPR isolated from rhizospheric soils of various plant and its effect on growth of radish (Raphanus sativus L.)

Abstract

Plant growth promoting rhizobacteria (PGPR) are commonly used as biofertilizers for agricultural crops. In addition, screening and selection of effective PGPR for a particular plant is necessary to meet plants specific need and condition. Present study was aimed to obtain PGPR isolates which are effective and compatible for Radish. A total of 15 PGPR isolates had been isolated from various plant roots and in vitro screening was done for different plant growth promotion activities. The result showed that 7 isolates were able to produce IAA at the range of 10.2-125.3 μg ml−1, 10 isolates were positive for phosphate solubilizing, 10 isolates had nitrogen fixing activities, 11 isolates were protease positive, 12 isolates were capable to produce ACC-deaminase enzyme, 7 isolates produced siderophore, 8 isolates produced HCN, and 9 isolates produced ammonia. The effect of 15 PGPR on Radish growth in the green house showed that all isolates had effects on increasing growth and tuber formation compared to control. Nine isolates significantly increased the wet weight shoot plants, the highest effect was reached by Kbm 10.3 isolate (168.5 g), while control only reached 81.5 g. Eight isolates had a significant effect on increasing tuber formation, the highest was Kbm 2.10 isolate (176 g), while control only reached 54.7 g. Kbm 10.3 and Kbm 2.10 isolates seemed to have the highest impact in promoting growth and tuber formation. Those two isolates were analyzed using 16s rDNA sequences, they have close evolutionary relationship with Bacillus megaterium (Kbm 2.10) and Paraburkholderia tropica (Kbm 10.3).

Identification and Characterization of Beneficial Soil Microbial Strains for the Formulation of Biofertilizers Based on Native Plant Growth-Promoting Microorganisms Isolated from Northern Mexico

“…Individual application also generated significant increases, but as previously mentioned, it is advisable to apply them as consortia to take advantage of their complementary effect and ensure their survival when applied in the field [ 9 , 11 , 33 ]. Other reports have also revealed significant improvements in the vegetative growth of broccoli and radish when using biofertilizers, similar to those obtained in these experiments [ 34 , 35 , 36 ]. Agustiyani et al [ 34 ] isolated fifteen bacterial isolates and performed in vitro screening for several PGP activities.…”

“…Other reports have also revealed significant improvements in the vegetative growth of broccoli and radish when using biofertilizers, similar to those obtained in these experiments [ 34 , 35 , 36 ]. Agustiyani et al [ 34 ] isolated fifteen bacterial isolates and performed in vitro screening for several PGP activities. They found that isolates were positive for IAA production (10.2–125.3 mg/L) (seven isolates), phosphate solubilization (ten isolates), nitrogen fixation (ten isolates), ACC (1-aminocyclopropane-1-carboxylate)-deaminase enzymatic activity (12 isolates), siderophore secretion (seven isolates), and ammonia production (nine isolates).…”

Isolation and Molecular Characterization of Plant Growth Promoting Rhizobacteria from Groundnut (Arachis Hypogaea L.) Rhizosphere

“…The potent PGPR KP3 produced IAA at a significantly higher levels, compared to IAA production reported in the literature. 20 The ammonia production by the PGPR indirectly affects plant growth and development. The PGPR nitrogenous materials of peptones break down into ammonia, which is released into the soil and used by plants as their nutrient source.…”

Isolation and Characterization of Plant Growth Promoting Rhizobacteria form Raphanus sativus (Radish)

“…Hashem et al [10], reported the activity of B. subtilis in the rhizosphere, Bacillus species are a significant type of rhizobacteria which can form spores that can survive in the soil for long period of time under adverse conditions and also it is a root colonizer increase crop productivity under conditions of biotic and abiotic stress. Agustiyani et al [11], they characterized the PGPR isolated from rhizospheric soils of various plant and checked its effect on growth of radish (Raphanus sativus L.), total 15 PGPR isolates were isolated from various plant roots and in vitro screening was done for different plant growth promotion activities. PGPR on Radish growth in the green house showed that all isolates had effects on increasing growth and tuber formation compared to control.…”