Characterization of pH-Dependent Poly(acrylic Acid) Complexation with Phospholipid Vesicles

Fujiwara, Mitsuko; Grubbs, Robert H.; Baldeschwieler, John D.

doi:10.1006/jcis.1996.4608

Cited by 64 publications

(55 citation statements)

References 8 publications

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“…This fact led the PSMA to complex with lipids through hydrogen bonding and hydrophobic interactions. In agreement with previous studies [2,[33][34][35][36], the results obtained herein demonstrate a pH-dependent association of PSMA and phosphatidylcholines. The collapse pressure values were higher than those of the pure phospholipids, suggesting that the incorporation of PSMA raised the intermolecular interaction at the interface upon compression.…”

Section: -Introductionsupporting

confidence: 93%

Polymer–lipid interactions: Biomimetic self-assembly behaviour and surface properties of poly(styrene-alt-maleic acid) with diacylphosphatidylcholines

Sáez-Martínez

Punyamoonwongsa

Tighe

2015

Reactive and Functional Polymers

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Section: -Introductionsupporting

confidence: 93%

Polymer–lipid interactions: Biomimetic self-assembly behaviour and surface properties of poly(styrene-alt-maleic acid) with diacylphosphatidylcholines

Sáez-Martínez

Punyamoonwongsa

Tighe

2015

Reactive and Functional Polymers

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“…The result showed about 4.0 mg of PAA in 20 mg of F127-PAA copolymer and the percentage of PAA in F127-PAA copolymer was about 20% (Table 1). More importantly, the pKa of the F127-PAA copolymer was about 4.8, similar to that of poly(acrylic acid) (pKa ¼ 4.5) 33 .…”

Section: Content Of Paa In the F127-paa Copolymersmentioning

confidence: 64%

Pluronic-poly (acrylic acid)-cysteine/Pluronic L121 mixed micelles improve the oral bioavailability of paclitaxel

Zhao

et al. 2013

Drug Development and Industrial Pharmacy

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The aim of the study is to synthesize a thiolated Pluronic copolymer, Pluronic-poly (acrylic acid)-cysteine copolymer, to construct a mixed micelle system with the Pluronic-poly (acrylic acid)-cysteine copolymer and Pluronic L121 (PL121) and to evaluate the potential of these mixed micelles as an oral drug delivery system for paclitaxel. Compared with Pluronic-poly (acrylic acid)-cysteine micelles, drug-loading capacity of Pluronic-poly (acrylic acid)-cysteine/PL121 mixed micelles was increased from 0.4 to 2.87%. In vitro release test indicated that Pluronic-poly (acrylic acid)-cysteine/PL121 mixed micelles exhibited a pH sensitivity. The permeability of drug-loaded micelles in the intestinal tract was studied with an in situ perfusion method in rats. The presence of verapamil and Pluronic both improved the intestinal permeability of paclitaxel, which further certified the inhibition effect of thiolated Pluronic on P-gp. In pharmacokinetic study, the area under the plasma concentration-time curve (AUC0→∞) of paclitaxel-loaded mixed micelles was four times greater than that of the paclitaxel solution (p < 0.05). In general, Pluronic-poly (acrylic acid)-cysteine/PL121 micelles were proven to be a potential oral drug delivery system for paclitaxel.

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“…The increase in surface binding at lower pH can be explained by the relatively higher hydro- phobicity (exposed cholesterol moieties) along with the lower ionic charge (protonated MAA units) of the copolymer, possibly increasing the interaction of the copolymer chains with phosphatidylcholine/cholesterol lipid bilayer. 74,80 Upon determining the copolymer composition displaying the highest binding to the plasma membrane-mimicking surface, the polymer−lipid interaction was further investigated in relation to lipid bilayer composition ( Figure 6). The interaction of the 2 mol % CMA copolymer with plasma or endosome membrane-mimicking lipid bilayer arrangements having different cholesterol contents (molar ratio of 0.42 and 0.80, respectively) were compared in a pH-dependent manner using SPR.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Well-Defined Cholesterol Polymers with pH-Controlled Membrane Switching Activity

Sevimli¹,

İnci

Zareie

et al. 2012

Biomacromolecules

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Cholesterol has been used as an effective component of therapeutic delivery systems because of its ability to cross cellular membranes. Considering this, welldefined copolymers of methacrylic acid and cholesteryl methacrylate, poly(methacrylic acid-co-cholesteryl methacrylate) P(MAA-co-CMA), were generated as potential delivery system components for pH-controlled intracellular delivery of therapeutics. Statistical copolymers with varying cholesterol contents (2, 4, and 8 mol %) were synthesized via reversible addition−fragmentation chain transfer (RAFT) polymerization. Dynamic light scattering (DLS) analysis showed that the hydrodynamic diameters of the copolymers in aqueous solutions ranged from 5 ± 0.3 to 7 ± 0.4 nm for the copolymers having 2 and 4 mol % CMA and 8 ± 1.1 to 13 ± 1.9 nm for the copolymer having 8 mol % CMA with increasing pH (pH 4.5−7.4). Atomic force microscopy (AFM) analysis revealed that the copolymer having 8 mol % CMA formed supramolecular assemblies while the copolymers having 2 and 4 mol % CMA existed as unimers in aqueous solution. The pH-responsive behavior of the copolymers was investigated via UV−visible spectroscopy revealing phase transitions at pH 3.9 for 2 mol % CMA, pH 4.7 for 4 mol % CMA, and pH 5.4 for 8 mol % CMA. Lipid bilayers and liposomes as models for cellular membranes were generated to probe their interactions with the synthesized copolymers. The interactions were determined in a pH-dependent manner (at pH 5.0 and 7.4) using surface plasmon resonance (SPR) spectroscopy and liposome leakage assay. Both the SPR analyses and liposome leakage assays indicated that the copolymer containing 2 mol % CMA displayed the greatest polymer−lipid interactions at pH 5.0, presenting the highest binding ability to the lipid bilayer surfaces, and also demonstrating the highest membrane destabilization activity. CellTiter−Blue assay showed that the copolymers did not affect the cell viability up to 30 μM over a period of 72 h.

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Characterization of pH-Dependent Poly(acrylic Acid) Complexation with Phospholipid Vesicles

Cited by 64 publications

References 8 publications

Polymer–lipid interactions: Biomimetic self-assembly behaviour and surface properties of poly(styrene-alt-maleic acid) with diacylphosphatidylcholines

Polymer–lipid interactions: Biomimetic self-assembly behaviour and surface properties of poly(styrene-alt-maleic acid) with diacylphosphatidylcholines

Pluronic-poly (acrylic acid)-cysteine/Pluronic L121 mixed micelles improve the oral bioavailability of paclitaxel

Well-Defined Cholesterol Polymers with pH-Controlled Membrane Switching Activity

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