Characterization of phenolic compounds in two novel lines of Pisum sativum L. along with their in vitro antioxidant potential

Nazir, Nausheen; Nisar, Mohammad; Ahmad, Sajjad; Wadood, Syed Fazal; Jan, Tour; Zahoor, Muhammad; Ahmad, Manzoor; Ullah, Abid

doi:10.1007/s11356-019-07065-y

Cited by 11 publications

(9 citation statements)

References 43 publications

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“…which exhibits strong antioxidant potential (Nazir et al 2018). Hydroxybenzoic acid, phloroglucinol, rutin, quercetin, and catechin derivatives possess strong antioxidant activities as reported by numerous investigators (Nazir et al 2019;Alarcón et al 2008;Iacopini et al 2008;Ramos et al 2013). Indeed, quercetin and rutin are often used as a standard in DPPH, ABTS, and other assays (Pourmorad et al 2006).…”

Section: Discussionmentioning

confidence: 97%

HPLC-UV characterization, anticholinesterase, and free radical-scavenging activities of Rosa moschata Herrm. leaves and fruits methanolic extracts

et al. 2020

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In the current research, we have investigated the antioxidant and cholinesterase inhibitory potentials of Rosa moschata Herrm. leaves and fruits methanolic extract (RmL.Ext and RmF.Ext) for the first time. To determine the rough estimate of phytochemical composition of extracts, the total phenolic and flavonoid contents were determined using standard methods. Moreover, the phenolic compounds present in the extracts were identified by HPLC-UV analysis. The cholinesterase activities were determined following the Ellman's assay. The antioxidant activities of extracts were investigated using DPPH and ABTS assays. Significant anticholinesterase potentials were exhibited by RmL.Ext with IC 50 of 40 and 110 µg/ml against AChE and BChE respectively. In addition, RmL.Ext also scavenged the DPPH (IC 50 = 360 µg/ml) and ABTS (IC 50 = 105 µg/ml) free radicals. Comparatively, the leaves extract was more potent than fruits extract. Malic acid, chlorogenic acid, quercetin, rutin, pyrogallol, mandelic acid, hydroxybenzoic acid, and morin were identified as the possible potent antioxidants present in extract. Based on the current results of the study, it may be inferred that RmL.Ext and RmF.Ext of R. moschata are rich in bioactive compounds responsible for the free radicals scavenging and cholinesterases inhibition and this plant may be a valuable candidate for the treatment of neurodegenerative disorders, like Alzheimer's disease. However, further investigation in terms of isolation of bioactive compounds and their screening of toxicological effects in animal models are needed to validate the observed results. Keywords Alzheimer's disease • Antioxidants • Flavonoids contents • Phenolic contents Abbreviation RmL.Ext Rosa moschata leaves extract RmF.Ext Rosa moschata fruit extract DPPH 2,2-Diphenyl-1-picrylhydrazyl ABTS 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt IC 50 Half maximal inhibitory concentration AChE Acetylcholinesterase enzyme BChE Butyrylcholinesterase enzyme BGH Botanical Garden Herbarium DAD Diode array detector HPLC High-performance liquid chromatography TPC Total phenolic content TFC Total flavonoid contents DTNB 5,5′-Dithiobis-(2-nitrobenzoic acid) Electronic supplementary material The online version of this article (

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Section: Discussionmentioning

confidence: 97%

HPLC-UV characterization, anticholinesterase, and free radical-scavenging activities of Rosa moschata Herrm. leaves and fruits methanolic extracts

et al. 2020

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“…Inhibition of these two enzymes are used as strategy to maintain the level of ACh in body which would consequently relieve the symptoms associated with AD, Dementia and Parkinson ’ s disease [ 8 ]. The consumption of edible plants/vegetables throughout the world has enormously increased as they are chief sources of phenolic compounds and even out of the five recommended drugs used for the treatment of AD, two are plant phytochemicals [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Chemical composition, in vitro antioxidant, anticholinesterase, and antidiabetic potential of essential oil of Elaeagnus umbellata Thunb

Nazir

Zahoor

Uddin

et al. 2021

BMC Complement Med Ther

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Background Elaeagnus umbellata Thunb. (autumn olive) is a high valued medicinal plant. It belongs to Elaeagnaceae family and is widely distributed in Himalayan regions of Pakistan. In the present study essential oil were extracted from the fruit of this plant and their antioxidant, anticholinesterase and antidiabetic potentials were also evaluated. Methods Essential oils were extracted from the fruit of E. umbellata using hydro-distillation method and were characterized by GC-MS. The extracted oil were tested for its antioxidant, anticholinesterase, and antidiabetic potentials using standard protocols. Results About 68 compounds were identified by GC-MS. The extracted oil exhibited a fairly high free radical scavenging activities against DPPH and ABTS radicals with IC50 values of 70 and 105 μg/mL respectively (for ascorbic acid, used as standard, the IC50 values were 32 and 29 μg/mL, respectively against the mentioned radicals). The essential oil also exhibited anticholinesterase activities with IC50 values of 48 and 90 μg/mL respectively against AChE and BChE (for galantamine used as standard, the IC50 values were 25 and 30 μg/mL respectively). The essential oil also exhibited antidiabetic potential with IC50 values of 120 and 110 μg/mL respectively against α-glucosidase and α-amylase (IC50 values for standard acarbose = 28 and 30 μg/mL respectively). Conclusion Essential oil extracted from the fruits of E. umbellata exhibited reasonable antioxidant, anticholinesterase, and antidiabetic potentials that could be used as alternative medicine in treating diabetes and neurodegenerative disorders. However, further studies are needed to isolate responsible compounds and evaluate the observed potential in animal models. Graphical abstract

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“…TPC was determined according to the Folin-Ciocalteu method with slight modifications [14]. First, 50 μL of each extract was added to 100 μL of Folin-Ciocalteu's reagent (50%).…”

Section: Total Phenolic Content (Tpc)mentioning

confidence: 99%

“…The TFC of the leaf extracts was assessed via an aluminum chloride colorimetric method [14]. First, 25 μL of 5% sodium nitrite solution was added to 50 μL of extract, after which the mixture was allowed to stand at RT for 5 min.…”

Section: Total Flavonoid Content (Tfc)mentioning

confidence: 99%

Comprehensive profiling of phytochemical compounds, antioxidant activities, anti-HepG2 cell proliferation, and cholinesterase inhibitory potential of Elaeagnus mollis leaf extracts

Kong

et al. 2020

PLoS ONE

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The aim of this work was to enrich the knowledge on the potential applications of Elaeagnus mollis leaf extracts. For this purpose, the bioactive compounds (phenolic, flavonoid, alkaloid, proanthocyanidin, chlorophyll and carotene content), antioxidant activity, anti-HepG2 cell proliferation, and cholinesterase inhibitory potential (AChE and BChE) of E. mollis leaves which obtained from different habitats were quantitatively analyzed using various solvents (water, methanol, ethanol, and n-hexane). The results showed that the methanol extracts exhibited the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and the water extracts showed the best antioxidant activity in the 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging activity, ferric reducing antioxidant power (FRAP), and reducing power (RP) assays. Moreover, the methanol extracts showed the best inhibitory activity against cholinesterase and HepG2 cancer cells. Correlation analysis revealed that the high antioxidant and anti-HepG2 cell proliferation activities were mainly attributed to the total phenolics, flavonoids, and proanthocyanidins while AChE inhibition was attributed to the total alkaloid and carotene content. The statistical results showed that the effect of habitats was lower than that of different solvents used. Additionally, the metabolic profiles of E. mollis leaves were evaluated using HPLC-ESI-Q TRAP-MS/MS, and a total of 1,017 chemical components were detected and classified into 23 classes. The organic acids and derivatives ranked the first, followed by flavone, amino acid and derivatives, and so on. In conclusion, the effects of different solvents were more significant than the effects of different habitats and the methanol extracts of E. mollis leaves could be used as an effective source of functional active components, provide benefits to physical health care and be applied to the food and pharmaceutical industries.

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Characterization of phenolic compounds in two novel lines of Pisum sativum L. along with their in vitro antioxidant potential

Cited by 11 publications

References 43 publications

HPLC-UV characterization, anticholinesterase, and free radical-scavenging activities of Rosa moschata Herrm. leaves and fruits methanolic extracts

HPLC-UV characterization, anticholinesterase, and free radical-scavenging activities of Rosa moschata Herrm. leaves and fruits methanolic extracts

Chemical composition, in vitro antioxidant, anticholinesterase, and antidiabetic potential of essential oil of Elaeagnus umbellata Thunb

Comprehensive profiling of phytochemical compounds, antioxidant activities, anti-HepG2 cell proliferation, and cholinesterase inhibitory potential of Elaeagnus mollis leaf extracts

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