Characterization of Phenotypes of Immune Cells and Cytokines Associated with Chronic Exposure to Premolis semirufa Caterpillar Bristles Extract

Abstract

The Brazilian moth Premolis semirufa (Walker, 1856), usually called pararama, is a parasite of the rubber Hevea genus. Contact with the bristles causes symptoms of acute inflammation. A chronic inflammatory reaction frequently occurs in individuals after multiple contacts, and this reaction is characterised by articular synovial membrane thickening with joint deformities, common characteristics of chronic synovitis. Extract from the bristles has been shown to induce an intense inflammatory response in a murine model, and this reaction was characterised by the presence of neutrophils in the paw tissues of injected mice and a strong, specific antibody response. There is not yet an effective treatment for incidents involving contact with pararama. In this study, we evaluated the phenotype of the immunological response and cytokine production in BALB/c mice subcutaneously injected in the footpad with P. semirufa bristle extract or sterile saline (control) seven times at 15 day intervals. An analysis of cells from the draining lymph node by flow cytometry showed that the absolute numbers of TCD4, TCD8 and B lymphocytes, as well as the expression of activation molecules, were higher in the extract-treated group. Furthermore, immunohistochemistry and immunofluorescence analyses showed a mixed inflammatory infiltrate composed of neutrophils and macrophages at the inoculation site. In addition, an analysis of paw cytokines showed elevated levels of IL-6, IL-12, IL-10, IL-17 and IL-23 after the 7th inoculation. In conclusion, these data provide evidence of pro-inflammatory changes in the phenotypes of immune cells and cytokine production in animals subjected to injections with an extract from Premolis semirufa bristles, which may explain the intense and prolonged inflammatory response that characterises this disorder.

Arthropod venom Hyaluronidases: biochemical properties and potential applications in medicine and biotechnology

“…The hyaluronidase plays a key role in the Pararama associated phalangeal periarthritis observed after the envenoming caused by the caterpillar Premolis semirufa [ 60 ]. The enzyme from the spider Hippasa partita indirectly potentiated the myotoxicity of VRV-PL-VIII myotoxin and the effect of hemorrhagic complex-I [ 16 ].…”

“…The composition of the venom is not well known and it varies among different species of caterpillars [ 95 ]. The presence of hyaluronidases has been reported in the venoms of Lonomia obliqua , Premolis semirufa and Megalopyge urens [ 18 , 19 , 60 ]. The hyaluronidase activity of the P. semirufa venom was measured in the presence of hyaluronan [ 60 ].…”

“…The presence of hyaluronidases has been reported in the venoms of Lonomia obliqua , Premolis semirufa and Megalopyge urens [ 18 , 19 , 60 ]. The hyaluronidase activity of the P. semirufa venom was measured in the presence of hyaluronan [ 60 ]. A hyaluronidase was suggested as the factor behind the Pararama associated phalangeal periarthritis, a serious public health problem among the Brazilian tappers (rubber plantation workers).…”

“…A hyaluronidase was suggested as the factor behind the Pararama associated phalangeal periarthritis, a serious public health problem among the Brazilian tappers (rubber plantation workers). It is a disease associated with joint immobilization, loss of the cartilage and bone structure and is known to be caused by the P. semirufa envenoming [ 60 ].…”

Population Explosions of Tiger Moth Lead to Lepidopterism Mimicking Infectious Fever Outbreaks

“…In fever patients, the knee joint disease was distressingly persisting and even a slight touch or movement was debilitating. TNF-α concentrations are correlated with the severity of the joint disease, because of the more complex interaction of immune modulators at the synovial membrane [ 13 , 36 ]. Based on these observations, we estimated levels of serum TNF-α concentrations, which showed significant difference between joint disease 287.7 ± 407.1 pg/mL ( n = 26) and healthy control groups 17.2 ± 3.5 pg/mL ( n = 10, P < 0.05).…”