Characterization of PhPRP1, a histidine domain arabinogalactan protein from Petunia hybrida pistils

Twomey, Megan C.; Brooks, Jenna K.; Corey, Jillaine M.; Singh-Cundy, Anu

doi:10.1016/j.jplph.2013.05.001

Cited by 7 publications

(9 citation statements)

References 16 publications

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“…The possible role of AGP epitopes in pollen tube growth is further reinforced by the fact that they could no longer be detected in either the suture line or the tissues surrounding it after pollen tube passage, while in unpollinated stigmas they were still present in the extracellular space of the suture line. A similar spatial and developmental pattern has been reported for the TTS AGP‐like protein in Nicotiana transmitting tissue within the style (Cheung et al, 1995; Wu et al, 2000; Twomey et al, 2013), and AGPs have also been localized in the stigmas and transmitting tissues of the style of a number of eudicots (Wu et al, 2000, 2001; Hancock et al, 2005; Coimbra et al, 2007; Suárez et al, 2012). Our controlled pollination experiments show that stigmatic receptivity in M. virginiana lasts for only a few hours and correlates with the spatiotemporal secretion of two AGP epitopes onto the stigma, thus suggesting that they could mark flower receptivity in this species.…”

Section: Discussionsupporting

confidence: 69%

“…But AGPs are also present in sporophytic tissues, especially those involved in the interaction with germinating pollen grains and growing pollen tubes. Thus, AGPs were initially identified biochemically in the stigmatic exudates of Nicotiana and Lilium at flower opening (Clarke et al, 1979; Gell et al, 1986; Bacic et al, 1988) and, more recently, in Petunia (Twomey et al, 2013). In apple flowers ( Malus domestica ), acquisition of stigmatic receptivity was shown to be mediated through the secretion of AGPs (Losada and Herrero, 2012).…”

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confidence: 99%

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Arabinogalactan proteins mark stigmatic receptivity in the protogynous flowers of Magnolia virginiana (Magnoliaceae)

Losada

Herrero

Hormaza

et al. 2014

American J of Botany

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These results show that the period of stigmatic receptivity is finely coordinated with the secretion of two arabinogalactan proteins on stigmas of flowers of M. virginiana. This first report of AGP presence in stigmatic tissues in a member of the magnoliids, together with recently described similar patterns in eudicots, monocots, and members of early-divergent lineages of flowering plants, suggests an ancient and widespread role for AGPs on stigmatic receptivity in angiosperms.

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Section: Discussionsupporting

confidence: 69%

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confidence: 99%

Arabinogalactan proteins mark stigmatic receptivity in the protogynous flowers of Magnolia virginiana (Magnoliaceae)

Losada

Herrero

Hormaza

et al. 2014

American J of Botany

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“…Then, several proteins very close to AGPNa3 were studied, for a review, see [11]. As examples, the following ones can be mentioned: Daucus carota DcAGP1 [12]; Arabidopsis thaliana AtAGP30 [13], and AtAGP31 (At1g28290) [14]; Capsicum annuum CaPRP1 [15]; Gossypium hirsutum GhAGP31 [16]; and Petunia hybrida PhPRP1 [17]. More recently, it appeared that the PAC domain could also be found alone, located at the N-terminus of the mature protein or associated with different types of domains, such as a Histidine-rich region, an O-glycosylated Proline/Hydroxyproline-rich domain, or an extensin domain [18,19].…”

Section: Introductionmentioning

confidence: 99%

The Cell Wall PAC (Proline-Rich, Arabinogalactan Proteins, Conserved Cysteines) Domain-Proteins Are Conserved in the Green Lineage

Nguyen-Kim

Clemente

Laimer

et al. 2020

IJMS

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Plant cell wall proteins play major roles during plant development and in response to environmental cues. A bioinformatic search for functional domains has allowed identifying the PAC domain (Proline-rich, Arabinogalactan proteins, conserved Cysteines) in several proteins (PDPs) identified in cell wall proteomes. This domain is assumed to interact with pectic polysaccharides and O-glycans and to contribute to non-covalent molecular scaffolds facilitating the remodeling of polysaccharidic networks during rapid cell expansion. In this work, the characteristics of the PAC domain are described in detail, including six conserved Cys residues, their spacing, and the predicted secondary structures. Modeling has been performed based on the crystal structure of a Plantago lanceolata PAC domain. The presence of β-sheets is assumed to ensure the correct folding of the PAC domain as a β-barrel with loop regions. We show that PDPs are present in early divergent organisms from the green lineage and in all land plants. PAC domains are associated with other types of domains: Histidine-rich, extensin, Proline-rich, or yet uncharacterized. The earliest divergent organisms having PDPs are Bryophytes. Like the complexity of the cell walls, the number and complexity of PDPs steadily increase during the evolution of the green lineage. The association of PAC domains with other domains suggests a neo-functionalization and different types of interactions with cell wall polymers

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“…Orthologs of TTS proteins were identified in Petunia hybrida by Twomey et al [22], who also demonstrated that the TTS proteins of N. tabacum are expressed in seedlings, roots, and leaves of tobacco. The P. hybrida ortholog ( PhPRP1 ) is likewise expressed in vegetative tissues, as well as in the transmitting tissue, which constitutes the pollen tube pathway in the central core of a solanaceous style.…”

Section: Introductionmentioning

confidence: 99%

“…The P. hybrida ortholog ( PhPRP1 ) is likewise expressed in vegetative tissues, as well as in the transmitting tissue, which constitutes the pollen tube pathway in the central core of a solanaceous style. Because TTS protein and its orthologous proteins are not unique to the transmitting tissue, Twomey et al [22] refer to TTS proteins and their orthologs as histidine-domain arabinogalactan proteins (HD-AGPs). HD-AGPs contain a histidine-rich domain not seen in any other family of arabinogalactan proteins (AGPs) described so far.…”

Section: Introductionmentioning

confidence: 99%

HD-AGPs as Speciation Genes: Positive Selection on a Proline-Rich Domain in Non-Hybridizing Species of Petunia, Solanum, and Nicotiana

Callaway¹,

Singh-Cundy²

2019

Plants

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Transmitting tissue-specific proteins (TTS proteins) are abundant in the extracellular matrix of Nicotiana pistils, and vital for optimal pollen tube growth and seed set. We have identified orthologs from several species in the Solanaceae, including Petunia axillaris axillaris and Petunia integrifolia. We refer to TTS proteins and their orthologs as histidine domain-arabinogalactan proteins (HD-APGs). HD-AGPs have distinctive domains, including a small histidine-rich region and a C-terminal PAC domain. Pairwise comparisons between HD-AGPs of 15 species belonging to Petunia, Nicotiana, and Solanum show that the his-domain and PAC domain are under purifying selection. In contrast, a proline-rich domain (HV2) is conserved among cross-hybridizing species, but variant in species-pairs that are reproductively isolated by post-pollination pre-fertilization reproductive barriers. In particular, variation in a tetrapeptide motif (XKPP) is systematically correlated with the presence of an interspecific reproductive barrier. Ka/Ks ratios are not informative at the infrageneric level, but the ratios reveal a clear signature of positive selection on two hypervariable domains (HV1 and HV2) when HD-AGPs from five solanaceous genera are compared. We propose that sequence divergence in the hypervariable domains of HD-AGPs reinforces sympatric speciation in incipient species that may have first diverged as a consequence of pollinator preferences or other ecological factors.

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Characterization of PhPRP1, a histidine domain arabinogalactan protein from Petunia hybrida pistils

Cited by 7 publications

References 16 publications

Arabinogalactan proteins mark stigmatic receptivity in the protogynous flowers of Magnolia virginiana (Magnoliaceae)

Arabinogalactan proteins mark stigmatic receptivity in the protogynous flowers of Magnolia virginiana (Magnoliaceae)

The Cell Wall PAC (Proline-Rich, Arabinogalactan Proteins, Conserved Cysteines) Domain-Proteins Are Conserved in the Green Lineage

HD-AGPs as Speciation Genes: Positive Selection on a Proline-Rich Domain in Non-Hybridizing Species of Petunia, Solanum, and Nicotiana

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