Characterization of phytochemical components of Ferula halophila extracts using HPLC-MS/MS and their pharmacological potentials: a multi-functional insight

Zengin, Gökhan; Uysal, Ahmet; Diuzheva, Alina; Güneş, Erdoğan; Jekő, József; Cziáky, Zoltán; Picot-Allain, Marie Carene Nancy; Mahomoodally, Mohamad Fawzi

doi:10.1016/j.jpba.2018.08.020

Cited by 65 publications

(31 citation statements)

References 53 publications

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“…In recent studies, F. halophila and F. elaeochytris methanol extracts displayed potent antioxidant capacities in comparison with apolar extracts [36,37]. Besides, in acetylcholinesterase inhibitory activity studies, the chloroform extracts of F. persica var.…”

Section: Discussionmentioning

confidence: 99%

Acetylcholinesterase inhibitory activities and LC-MS analysis of the antioxidant Ferula caspica M. Bieb. and F. halophila Peşmen extracts

Kahraman¹,

Baysal²,

Cankaya³

et al. 2019

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The aim of the current study is to evaluate the biological activity of F. caspica M. Bieb. which is used for the treatment of diabetes in traditional Turkish medicine and to compare with F. halophila Peşmen. Antioxidant capacities and total phenolic contents of the plants' extracts were tested by DPPH radical scavenging assay and Folin Ciocalteu assay, respectively. Acetylcholinesterase inhibitory activities of the extracts were investigated with the aim of indicating whether there was a correlation with their antioxidant capacities. Different quinic acid and flavonoid derivatives which may be responsible for the activities of the extracts were detected using LC-MS screening in the methanol extracts. The methanol extract from the aerial parts of F. caspica was found to show most antioxidant capacity and acetylcholinesterase inhibitory activity.

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Section: Discussionmentioning

confidence: 99%

Acetylcholinesterase inhibitory activities and LC-MS analysis of the antioxidant Ferula caspica M. Bieb. and F. halophila Peşmen extracts

Kahraman¹,

Baysal²,

Cankaya³

et al. 2019

jrp

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“…The accurate description of chromatographic method is reported in our previous published paper [20]. The Thermo Scientific Xcalibur 3.1 (Thermo Scientific, Waltham, MA, USA) and TraceFinder 3.1 (Thermo Scientific, Waltham, MA, USA) softwares were used to record and evaluate data [22]. The concentrations were 5 mg/mL and 2 µL was injected in every run.…”

Section: Liquid Chromatography Analysismentioning

confidence: 99%

“…The experiments were approved by Local Ethical Committee (University "G. d'Annunzio" of Chieti-Pescara) and Italian Health Ministry (Italian Health Ministry authorization N. F4738.N.XTQ, delivered on 11 November 2018). Rats were sacrificed by CO 2 inhalation (100% CO 2 at a flow rate of 20% of the chamber volume per min), and explanted colon specimens were stimulated with E. coli lipopolysaccharide (LPS), an ex vivo experimental model of ulcerative colitis [22]. In parallel, colon tissues were treated with Artemisia extracts (100 µg/mL) and essential oil (0.1 µL/mL).…”

Section: Ex Vivo Studiesmentioning

confidence: 99%

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Qualitative Fingerprint Analysis and Multidirectional Assessment of Different Crude Extracts and Essential Oil from Wild Artemisia santonicum L.

et al. 2019

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Artemisia species are used as folk medicines in several countries. This work was aimed to shed more light on the effect of methanol, water, ethyl acetate extracts, and essential oil (EO) of A. santonicum on selected enzymes (cholinesterase, tyrosinase α-amylase, and α-glucosidase) as well of their antioxidant and pharmacological effects. The chemical profile of the essential oil was determined using gas chromatography coupled to mass spectrometry (GC-MS) analysis, while the extracts were chemically characterized by high performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Forty-nine constituents were identified and camphor (36.6%), 1,8-cineole (10.2%), α-thujone (10.1%), borneol (4.5%), and β-thujone (3.6%) were the major components. Overall, 45, 74, and 67 components were identified from the ethyl acetate, methanol, and water extracts, respectively. The EO and extracts showed significant antioxidant properties, in a cell-free model; particularly, methanol and water extracts revealed promising sources of antioxidant compounds. Additionally, we evaluated protective effects of EO and extracts in isolated rat colon tissue challenged with lipopolysaccharide (LPS), as an ex vivo model of colon inflammation, and human colon cancer HCT116 cell line. Particularly, we observed that, among all tested samples, A. santonicum ethyl acetate displayed the best pharmacological profile, being able to blunt LPS-induced levels of all tested biomarkers of inflammation and oxidative stress, including colon nitrites, lactate dehydrogenase, prostaglandin E2, and serotonin. Additionally, this extract was also able to reduce HCT116 cell viability, thus suggesting potential antiproliferative effects against colon cancer cells. Based on our results, A. santonicum has great potential for developing novel functional agents including pharmaceuticals, cosmeceuticals, and nutraceuticals.

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“…Antioxidants from herbal sources play a vital role in protection from various health disorders such as skin problems, diabetes mellitus, different types of cancers, Parkinson's (PD), Alzheimer's disease (AD) and oxidative stress [3]. Recently, the extracts and isolated compounds from herbal sources have been gaining more attention as a source of inhibitors for several enzymes involved in different clinical conditions [4]. Incidents of Alzheimer's disease, which can be linked to aging, is almost going to reach 81.1 million in 2040 [5].…”

Section: Introductionmentioning

confidence: 99%

Secondary Metabolites Profiling, Biological Activities and Computational Studies of Abutilon figarianum Webb (Malvaceae)

et al. 2020

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This research endeavors to inspect the chemical and biological profiling of methanol and dichloromethane (DCM) extracts prepared from Abutilon figarianum Webb. Total bioactive constituents and secondary metabolites were assessed via ultra-high performance liquid chromatography (UHPLC-MS). Biological effects were evaluated via antioxidant and enzymes inhibitory assays. The methanol extract was able to give the highest phenolic (51.92 mg GAE/g extract) and flavonoid (72.59 mg QE/g extract) contents and was found to contain 11 bioactive metabolites, including flavonoid, alkaloid, phenolic and fatty acid derivatives, as accessed by UHPLC-MS analysis. Similarly, the phytochemical profiling of the DCM extract tentatively identified the 12 different secondary metabolites, most of these were fatty acid derivatives. The methanol extract was most active in the radical scavenging, reducing power and total antioxidant power assays, while dichloromethane extract showed the highest metal chelating activity. For enzyme inhibition, the DCM extract showed the highest activity against cholinesterases, glucosidase and amylase, whereas methanol extract was most active against tyrosinase. Docking studies have supported the observed biological activity, where isobergapten showed higher activity against tyrosinase (−7.63 kcal/mol) with inhibition constant (2.55 µM), as opposed to other enzymes. The observed antioxidant and inhibitory potentials of A. figarianum against the studied enzymes tend to endorse this plant as a prospective source of bioactive phytochemicals.

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Characterization of phytochemical components of Ferula halophila extracts using HPLC-MS/MS and their pharmacological potentials: a multi-functional insight

Cited by 65 publications

References 53 publications

Acetylcholinesterase inhibitory activities and LC-MS analysis of the antioxidant Ferula caspica M. Bieb. and F. halophila Peşmen extracts

Acetylcholinesterase inhibitory activities and LC-MS analysis of the antioxidant Ferula caspica M. Bieb. and F. halophila Peşmen extracts

Qualitative Fingerprint Analysis and Multidirectional Assessment of Different Crude Extracts and Essential Oil from Wild Artemisia santonicum L.

Secondary Metabolites Profiling, Biological Activities and Computational Studies of Abutilon figarianum Webb (Malvaceae)

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