Characterization of pili protein with molecular mass of 85 kDa Escherichia coli as an adhesin and a hemagglutinin

Abstract

Background: Escherichia coli (E. coli) is a rod-shaped gram-negative bacterium included in Multi Drug Resistant (MDR) bacteria. There are several strategies in place to prevent and treat E. coli bacterial infections to reduce some the incidences of MDR. One of them is the development of a protein-based vaccine. Pili is one of virulence factors in E. coli surface proteins that can mediate attachments of bacteria to host cells (adhesin or hemagglutinin) and can be used as protein-based vaccine candidates. Objective: This study aims to determine characterization of pili protein with molecular mass of 85 kDa Escherichia coli bacteria as an adhesin and a hemagglutinin. Methods: This study is a true experimental laboratories and a descriptive study to determine roles of pili protein with molecular mass of 85 kDa as an adhesin and a hemagglutinin. Samples used in this study were a stock of E. coli in the Microbiology Laboratory Faculty of Medicine, Universitas Negeri Jember (UNEJ). A hemagglutination test in this study used mouse erythrocytes while an adhesion test used mouse enterocytes. Isolation and purification of E. coli pili protein, isolation of mouse erythrocytes, isolation of mouse enterocytes, the hemagglutination test and the adhesion test were methods in this study. A data analysis with a correlation-regression was used to determine relationships between the adhesion index and titer pili 85 kDa with a limit of significance, 0.05 (p <0.05). Results: The hemagglutination test showed no ability of agglutination with the formation of red aggregate points starting from the first dilution. An analysis with Pearson correlation indicated a relationship between titer pili 85 kDa E. coli and adhesion index (p = 0.009; R = -0.921). Conclusion: Pili protein 85 kDa E. coli plays a role as an adhesin protein and do not have a role as a hemagglutinin protein. Latar Belakang: Escherichia coli (E. coli) merupakan bakteri Gram negatif yang termasuk dalam bakteriMulti Drug Resistant (MDR). Saat ini ada beberapa strategi dilakukan untuk mencegah dan mengobati infeksi bakteri E. coli sehingga mengurangi angka terjadinya MDR. Salah satunya dengan pengembangan vaksin berbasis protein. Pili merupakan salah satu faktor virulensi pada protein permukaan E. coli yang dapat memperantarai perlekatan bakteri dengan sel inang (adhesin atau hemaglutinin) dan dapat dijadikan kandidat vaksin berbasis protein. Tujuan: Penelitian ini bertujuan untuk mengetahui peran pili 85 kDa E. coli sebagai protein adhesin dan hemaglutinin.

Increased levels of IL-10 in the spleen after induction of pili protein 65.5 kDa Klebsiella pneumoniae

“…Although there is a lot of evidence that supports the protection of antibodies against polysaccharide capsules, the large number of types causes vaccines from this virulence factor to be less feasible, so it is necessary to develop vaccines from other virulence factors such as fimbriae/pili (Arato et al, 2021;Choi et al, 2020;Lundberg et al, 2012). Our previous research showed that the pili protein K. pneumoniae 65.5 kDa exhibits immunogenic properties, which can hypatinate erythrocytes and can act as adhesin (Agustina et al, 2014(Agustina et al, , 2020Kurniawati et al, 2021;Thanassi et al, 2012), as well as being able to induce cytokines as a sign of the emergence of an immune response (in editing). The cytokine used in this study was interleukin-10 (IL-10).…”