Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of<i>Salmonella typhimurium</i>phage type 204c isolated in Britain

Threlfall, E. J.; Rowe, B.; Ferguson, J. L.; Ward, L. R.

doi:10.1017/s0022172400063609

Cited by 226 publications

(155 citation statements)

References 6 publications

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“…Additional strains of PT15 are also listed in Table 1 [8] and visualized after electrophoresis at 150 V for 2 5 h on vertical 0-7 % (w/v) Tris-borate agarose gels (Sigma, Type II, Mledium EFO). Plasmids were sized in relation to plasmids of known molecular weights carried in strains 39R861 [12] and V517 [13]. Restriction Factors governing the choice of oligosequence were: (i) about 20-30 base pairs (bp) in length; (ii) GC-rich ends, especially 3'; (iii) no hairpins or internal repeats.…”

Section: Bacteriamentioning

confidence: 99%

Molecular characterization of plasmids in Salmonella enteritidis phage types

et al. 1993

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SUMMARYPlasmids in selected type strains of 26 of the Salmonella enteritidis phage types have been characterized by restriction enzyme fingerprinting and by DNA-DNA hybridization with oligonucleotide probes for Salmonella plasmid virulence (Spv) genes. With one exception, the fingerprints of the 38 MDa plasmids studied were homogeneous but there was heterogeneity in the fingerprints of 59 MDa plasmids found in 4 of the type strains. However all 38 MDa and 59 MDa plasmids were related as was a 45 MDa plasmid identified in the type strain of phage type 19. A 3-5 kb fragment homologous to SpvC was conserved in Hind III digests of all 38 MDa and 59 MDa plasmids, and in the related 45 MDa plasmid. In contrast a 65 MDa plasmid found in the type strain of phage type 10 was not related to these three plasmid molecular weight groups and did not carry the SpvC gene.

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Section: Bacteriamentioning

confidence: 99%

Molecular characterization of plasmids in Salmonella enteritidis phage types

et al. 1993

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“…Apramycin-resistant E. coli and K. pneunioniae isolated from humans in the UK have also been reported [7]. An increasing prevalence of resistance to gentamicin has been found in S. typhirnuriun2 phage type 204c in humnans [8] and it has been suggested that use of apramycin in animals has selected for resistant salmonella isolates which then infect man [6].…”

Section: In'trodu( Ti()nmentioning

confidence: 99%

“…DNA was separated by electrophoresis in 0 7 % agarose gels and molecular weights of plasmnids were determined by cotmparison with four reference plasmids carried in E. coli strain 39R861 [6].…”

Section: Conjuiyationsmentioning

confidence: 99%

Human isolates of apramycin-resistant Escherichia coli which contain the genes for the AAC(3)IV enzyme

et al. 1993

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SUMIMIARYGentamicin-resistant Ekscherichia coli isolated at different periods from patients in two hospitals were tested for resistance to the aminoglycoside antibiotic apramycin. Twenty-four of 93 (26%) gentamicin-resistant isolates collected from the Royal Liverpool Hospital between 1981 and 1990 were resistant to apramycin. Thirteen isolates were highly resistant to apramycin (ininimal inhibitory concentration (MIC) > 1024 ,ug/ml). were also resistant to gentamicin. netilmicin and tobramtycin. and hybridized with a DNA probe derived from the amninoglycoside acetyltransferase (3)1N (AAC(3)IN') gene. The proportion of gentamicinresistant isolates which had high level resistance to apramycin increased from 7 % in 1981-5 to 24% in 1986-90.Twelve gentamicin-resistant E. coli from Guy's and St Thomas's Hospital isolated between 1977 and 1980 were also tested for resistance to apramycin. For five of these isolates the M1ICs of aprainycin -was 32-256 ,/g/ml. None was shown to have a conjugative plasmid carryTing resistance to apramycin and only one hybridizedc with the DNA probe for the AA(1(3)IV enzyme. IN'TRODU( TI()NApramycin

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“…Samples were analysed by electrophoresis at 100 V for 2-2-5 h on 0-6 % horizontal agarose gels (w/v, Sigma, Type II medium EEO), using a model H5 horizontal gel apparatus (Gibco/BRL), and were sized in relation to plasmids carried in the Escherichia coli strains 39R861 [11] and V517 [12], as previously described [7].…”

Section: Bacterial Strainsmentioning

confidence: 99%

Use of plasmid profile typing for surveillance ofSalmonella enteritidisphage type 4 from humans, poultry and eggs

et al. 1994

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SUMMARYPlasmids were found in 1022 of 1089 (94%) of drug-sensitive strains of Salmonella enteritidis phage type 4 from humans (sporadic and outbreak cases), poultry (chickens) and eggs in England and Wales in the 5-year period 1988-92 and 25 plasmid profile patterns were identified. Strains characterized by a single plasmid of 38 MDa predominated (= plasmid profile type SE 38), comprising over 90% of isolates from humans, 70% from poultry and 92 % from eggs. Eleven profile types were identified in strains from humans, 21 in strains from poultry and 3 in strains from eggs. Eight of the 11 patterns identified in human isolates were found in strains from poultry and 2 in strains from eggs. In contrast 15 patterns seen in poultry were not found in strains from humans. Four percent of strains from humans and 13 % from poultry did not carry the 38 MDa plasmid but all strains from eggs were found to carry this plasmid. The second most common profile type in strains isolated between 1981 and 1988 was not identified in strains isolated from 1988-92. It is concluded that plasmid profile typing is a useful method for rapid differentiation within phage type 4 of S. enteritidis but that methods which can discriminate within the predominant profile type, SE 38, are now required.

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Characterization of plasmids conferring resistance to gentamicin and apramycin in strains ofSalmonella typhimuriumphage type 204c isolated in Britain

Cited by 226 publications

References 6 publications

Molecular characterization of plasmids in Salmonella enteritidis phage types

Molecular characterization of plasmids in Salmonella enteritidis phage types

Human isolates of apramycin-resistant Escherichia coli which contain the genes for the AAC(3)IV enzyme

Use of plasmid profile typing for surveillance ofSalmonella enteritidisphage type 4 from humans, poultry and eggs

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