Characterization of Platelet-Rich Plasma/Gellan Gum Hydrogel Composite for Biological Performance to Induce Chondrogenesis from Adipose-Derived Stem Cells

Preparation and Characterization of Gellan Gum Hydrogel as Therapeutic Protein Delivery for Wound Healing

“…However, over time, cells were seen to be growing underneath the hydrogels leading to increased cell viability, especially in those wells incubated with GG hydrogel incorporated with a higher concentration of FBS. The increased cell viability effect on cells over time was also observed in previous research that used the combination of gellan gum and other therapeutic protein source like platelet-rich plasma [13].…”

“…Based on Table 2, the C, H, and N contents of GG were 32.25%, 5.46%, and 0% respectively. This is expected as GG it is a type of polysaccharide [13], which generally consists of elements C, H, and O [19,20]. Meanwhile, FBS showed 40.75% C, 5.460% H, and 5.321% N. The higher nitrogen content in FBS is most probably due to the presence of various protein components that it contains [21].…”

“…Gellan gum (GG), a polysaccharide synthesized by a non-pathogenic gram-negative bacterium called Pseudomonas elodea, is one of the naturally occurring polymers that can be made into a hydrogel [12]. GG hydrogel is known to be biocompatible and able to change its mechanical properties and degradation profile depending on the degree and types of crosslinking it contains [13]. The use of GG hydrogel has attracted a lot of interest to be a potential carrier in controlled release studies [14].…”

“…ofloxacin), lavender oil, and others, for wound healing purposes [15]. The combination of GG with therapeutic proteins, like platelet derivatives, has been found to enhance the micro-environment for chondrogenesis of adipose-derived stem cells in vitro [13]. Hence, the use of GG hydrogel may be worth to be investigated as a potential scaffold for therapeutic proteins to provide an alternative for wound regeneration.…”