Characterization of polymers by size exclusion chromatography using multiple detection. Determination of the Mark-Houwink constants for Pullulan 100

Sommermeyer, K.; Cech, Franz; Pfitzer, E.

doi:10.1007/bf02316438

Cited by 9 publications

(4 citation statements)

References 2 publications

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“…Prior to analysis, the liquid samples of Venofundin and VitaHES were freeze dried and the resulting content of sodium chloride was taken into consideration accordingly. Mean Mw, Mw distribution, the Mark–Houwink constants K as well as free and esterified phosphate were determined as described by Sommermeyer 10 and Sommermeyer et al 11 …”

Section: Methodsmentioning

confidence: 99%

Differences in chemical structures between waxy maize‐ and potato starch‐based hydroxyethyl starch volume therapeutics

Sommermeyer¹,

Cech²,

Schossow³

2007

Transfus Alt Transfus Med

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TATM S U M M A R YHydroxyethyl starch plasma volume expanders originating from different plant sources may have different physicochemical and clinical properties. We therefore compared the physicochemical properties of two mediummolecular-weight hydroxyethyl starch (HES) plasma volume expanders with a declared average molecular weight of 130 kDa and molar substitutions of 0.4 and 0.42, respectively, derived from waxy maize starch and potato starch, respectively. For HES based on waxy maize starch, we could demonstrate a higher mean degree of branching and a much lower degree of esterification with phosphoric acid, compared with the potato starch-based product. This is in accordance with the Mark-Houwink plots of potato starch-based HES showing higher values of the limiting viscosity over the whole molecular weight range in comparison with waxy maize starch-based HES. The relevance of these data on pharmacological properties is discussed. Waxy maize-and potato starch-based hydroxyethyl starch plasma volume expanders differ in their chemical fine structure. Their pharmacological and clinical equivalence remains to be proven with further studies.

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Section: Methodsmentioning

confidence: 99%

Differences in chemical structures between waxy maize‐ and potato starch‐based hydroxyethyl starch volume therapeutics

Sommermeyer¹,

Cech²,

Schossow³

2007

Transfus Alt Transfus Med

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“…39 We see that this ideal mode is described by equations (17) and (19). We have used this approach to venfy the universal calibration concept for several coil-like polymers, such as poly(ethy1 methacrylate), poly(viny1 acetate), and some statistical and block copolymers.…”

Section: Hydrodynamic Volume Calculation and Universal Calibration Comentioning

confidence: 97%

“…Using definition (1 1) and universal calibration hypothesis (19) we obtain from equations (21) -(23) for each slice i of a polymer distribution Usually, it is not an easy matter to find published v values for different wavelengths. Equation (24) allows calculating refractive index increments of each fraction of a polymer for one photometer, if the corresponding values for another with different wavelength are known and the universal calibration curve log Hst versus V is established.…”

Section: Hydrodynamic Volume Calculation and System Parametersmentioning

confidence: 99%

Data Reduction in Multidetector Size Exclusion Chromatography

Brun

1998

Journal of Liquid Chromatography & Related Technologies

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A comprehensive analysis of multidetector size exclusion chromatography (SEC) is presented. The system examined consists of differential refiactometer, capillary viscometer, and light-scattering detector combination. New approaches for interpreting multidetector data have been developed. The first one consists in eliminating the concentration chromatogram from the data reduction process. The traces of two molecular-weightsensitive detectors alone allow calculating molecular weight and intrinsic viscosity distributions of an unknown polymer, assuming the validity of the universal calibration concept for this polymer.This approach can dramatically improve the characterization of polymers with long high-molecular-weight tails, when small amounts of high-molecular-weight fractions are detected by the light-scattering and viscometer detectors, but the concentration is too low to be registered by the refractometer.Another opportunity discovered is the calculation of the hydrodynamic volume of each fraction of a chromatographed polymer across its distribution, using measured signals from three on-line detectors. This calculation can be performed for any complex polymers, e.g., copolymers or oligomers, even in situations when refractive index increments of molecules depend 1979

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“…It was reported that the branched macromolecules led to deterioration of the service properties. An SEC system was outlined using multiple detection by LALLS and a concentration detector along with a differential viscometer (60). This combination of three detectors permits the determination of Mw, Mn, viscosity average molecular weight, and the Mark-Houwink constants.…”

Section: Gel Permeation and Size Exclusion Chromatography (Gpc Sec)mentioning

confidence: 99%

Pesticides

Sherma¹

1989

Anal. Chem.

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Characterization of polymers by size exclusion chromatography using multiple detection. Determination of the Mark-Houwink constants for Pullulan 100

Cited by 9 publications

References 2 publications

Differences in chemical structures between waxy maize‐ and potato starch‐based hydroxyethyl starch volume therapeutics

Differences in chemical structures between waxy maize‐ and potato starch‐based hydroxyethyl starch volume therapeutics

Data Reduction in Multidetector Size Exclusion Chromatography

Pesticides

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