Characterization of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg Roots and their effects on antioxidant activity and H2O2-induced oxidative damage in RAW 264.7 cells

Abstract

This work presents an investigation on the composition and structure of polysaccharides from the roots of Tetrastigma hemsleyanum (THP) and its associated antioxidant activity. It further explores the protective effect of THP on RAW264.7 cells against cytotoxicity induced by H2O2. Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. Furthermore, gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS) was employed to deduce the relative molecular mass (Mw) of the polysaccharide, which was 177.1 ± 1.8 kDa. Fourier transform infrared spectroscopy (FT-IR) and Congo red binding assay highlighted that the THP had a steady α-triple helix conformation. Similarly, assays of antioxidant activity disclosed that THP had reasonable concentration-dependent hydroxyl radical and superoxide radical scavenging activities, peroxidation inhibition ability and ferrous ion chelating potency, in addition to a significant 1,1-diphenyl-2-picrylhydrazyl radical scavenging capacity. Moreover, THP could protect RAW264.7 cells against H2O2-induced cytotoxicity by decreasing intracellular ROS levels, reducing catalase (CAT) and superoxide dismutase (SOD) activities, increasing lactate dehydrogenase (LDH) activity and increment in malondialdehyde (MDA) level. Data retrieved from the in vitro models explicitly established the antioxidant capability of polysaccharides from T. hemsleyanum root extracts.

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

“…The high-level structures are divided into secondary, tertiary and quaternary structures. The secondary structure refers to the regular conformation formed by the hydrogen bond; the tertiary structure and the quaternary structure refer to the regular spatial conformation resulting from the non-covalent interaction between saccharide units based on secondary structure [ 35 , 77 , 78 ].…”

Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves

“…The above results were consistent with the infrared spectrum analysis results. Refer to 1 D/ 2 D‐NMR spectrum and relevant literature data (Hajji et al., 2019; Huang et al., 2021; Yu et al., 2018; Yuan et al., 2016; Zhu et al., 2019), the chemical displacement values of C 2 –C 5 in each connection mode were assigned as shown in Table 2.…”

Quality Evaluation of Tetrastigmae Radix from Two Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis

“…Procyanidin B2 ( 30 ) and catechin ( 33 ) are flavonoids. The flavonoid constituents of SYQ have antitumor [ 5 ] and antioxidant [ 7 ] effects, and these constituents may provide a new idea for the study of the antitumor activity of SYQ from different origins. Based on the r i values of the samples ( Table 3 ), the overall quality of ES was better than WS, indicating that different geographical regions can influence the accumulation of bioactive constituents.…”

“…Modern phytochemistry shows that SYQ contains a variety of active ingredients, including flavonoids, phenolic acids, nucleosides, amino acids, polysaccharides, triterpenes, steroids, alkaloids, and other constituents [ 3 ]. Modern pharmacological research on SYQ has a variety of pharmacological effects, including antitumor, antioxidant, anti-inflammatory, antibacterial, antipyretic, analgesic, immunomodulatory, and other activities [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. Among the total phenolic acids and total flavonoids of SYQ, rutin, isoquercitrin, nicotifiorin, and astragalin are the main substances, for which metabolites in the body could explain some of the antioxidant biomarkers [ 9 ].…”