Characterization of polysaccharides with antioxidant and immunological activities from Rhizoma Acori Tatarinowii

Zhang, Wuxia; Xu, Dan; Wang, Tingting; Chen, Lu; Duan, Jinyou

doi:10.1016/j.carbpol.2015.07.018

Cited by 61 publications

(18 citation statements)

References 51 publications

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“…Since juglone is a reactive oxygen species generator 45 , it gave us a notion that DXN could reduce Aβ toxicity through anti-oxidant activity. It agrees with the previous work that components of DXN, Vj and Rhizoma Acori tatarinowii possess high antioxidant activity 14 , 46 , 47 .…”

Section: Discussionsupporting

confidence: 93%

Dianxianning improved amyloid β-induced pathological characteristics partially through DAF-2/DAF-16 insulin like pathway in transgenic C. elegans

Zhi

Wang

Yang

et al. 2017

Sci Rep

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Dianxianning (DXN) is a traditional Chinese formula, and has been approved in China for treating epilepsy since 1996. Here anti-Alzheimer’s disease activity of DXN has been reported. DXN improved AD-like symptoms of paralysis and 5-HT sensitivity of transgenic Aβ1-42 C. elegans. In worms, DXN significantly increased Aβ monomers and decreased the toxic Aβ oligomers, thus reducing Aβ toxicity. DXN significantly suppressed the expression of hsp-16.2 induced by juglone, and up-regulated sod-3 expression. These results indicated that DXN increased stress resistance and protected C. elegans against oxidative stress. Furthermore, DXN could significantly promote DAF-16 nuclear translocation, but it did not activate SKN-1. The inhibitory effect of DXN on the Aβ toxicity was significantly reverted by daf-16 RNAi, rather than skn-1 RNAi or hsf-1 RNAi. These results indicated that DAF-16 is at least partially required for the anti-AD effect of DXN. In conclusion, DXN improved Aβ-induced pathological characteristics partially through DAF-2/DAF-16 insulin like pathway in transgenic worms. Together with our data obtained by Morris water maze test, the results showed that DXN markedly ameliorated cognitive performance impairment induced by scopolamine in mice. All the results support that DXN is a potential drug candidate to treat Alzheimer’s diseases.

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Section: Discussionsupporting

confidence: 93%

Dianxianning improved amyloid β-induced pathological characteristics partially through DAF-2/DAF-16 insulin like pathway in transgenic C. elegans

Zhi

Wang

Yang

et al. 2017

Sci Rep

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“…The IC 50 values of SCP and ULLP were 1.81% ± 0.07% and 1.66% ± 0.09% mg/mL, respectively, indicating that ULLP and SCP had good effects on hydroxyl radical scavenging abilities. Previous reports demonstrated that some plant polysaccharides, such as Fragrance and Rhizoma acori tatarinowii , with their hydroxyl radical scavenging abilities of 81.3%, at 20 mg/mL, and IC 50 value at 2.028 mg/mL, respectively [19,42], are significantly lower than the removal of hydroxyl radicals of SCP and ULLP. While the IC 50 of the other fractions (GLP and DAP) cannot be determined within the test dosage range (1–4 mg/L), which indicates that DAP and GLP have lower effect than SCP and ULLP on the removal of hydroxyl radicals, this may be due to GLP and DAP molecular weights being higher than that of SCP and ULLP.…”

Section: Discussionmentioning

confidence: 99%

Extraction, Structural Characterization, and Potential Antioxidant Activity of the Polysaccharides from Four Seaweeds

Chen

et al. 2016

IJMS

112

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Four seaweed polysaccharides were extracted from Sarcodia ceylonensis, Ulva lactuca L., Gracilaria lemaneiformis, and Durvillaea antarctica, respectively, by microwave-assisted extraction. The effect of three significant variables (extraction time, extraction temperature, and the ratio of water to raw material) on the process for extracting polysaccharides was investigated, along with the optimization of the extraction using the response surface method (RSM) with a Box–Behnken design. The polysaccharide structure, monosaccharide composition, degree of sulfation, and molecular weight (MW) distribution were analyzed by infrared (IR) spectrometry, gas chromatography (GC), and high-performance gel permeation chromatography (HPGPC). IR spectrometry showed that Sarcodia ceylonensis polysaccharide (SCP), Ulva lactuca L. polysaccharide (ULLP), and Durvillaea antarctica polysaccharide (DAP) were all sulfated polysaccharides and, except Gracilaria lemaneiformis polysaccharide (GLP), all belong to β-pyranosidic polysaccharides. The average molecular weight (MW) of SCP, ULLP, GLP, and DAP was 466, 404, 591, and 482 kDa, respectively. The quantitative and comparative results with external standards indicated that the main monosaccharide in SCP and ULLP was mannose; and GLP and DAP were mainly composed of galactose and glucose, respectively. Then the in vitro antioxidant activity of all of the polysaccharides was evaluated using different assays—2,2–azino –bis (3-ethylbenzthiazoline-6- sulfonate) (ABTS), hydroxyl radical, nitrite scavenging capacity, and reducing power—and the relationship between their antioxidant activity and chemical characteristics were also examined. ULLP presented the highest ABTS radical scavenging activity; ULLP, SCP and DAP also showed a strong effect on the ABTS radical scavenging activity. SCP and ULLP exhibited excellent hydroxyl radical scavenging activities, about 83.33% ± 2.31% and 80.07% ± 2.17%, respectively, at 4 mg/mL. The reducing power of DAP was relatively more pronounced than that of the three other polysaccharides. However, the nitrite scavenging activities of the four seaweed polysaccharides were weaker than other antioxidant activity (ABTS), hydroxyl radical scavenging capacity, and reducing power. In addition, GLP exhibited lower activities than the other three samples in all of the tests for the antioxidant activity.

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“…Under these conditions, the content of polysaccharides in red peony root could reach (10.11 ± 0.52)% 13 . The optimal conditions for microwave-assisted extraction of Bletilla striata polysaccharide were as follows: the ratio of liquid to material was 75:1, the extraction temperature was 50 °C, the extraction time was 15 min, the microwave power was 700 watts, and the extraction times were two times 14 . The extraction rate of B. bletilla polysaccharide was 25.80%, which was better than ultrasonic extraction rate of 13.52%.…”

Section: Extraction Of Polysaccharide From Traditional Chinese Medicinementioning

confidence: 99%

Extraction and derivatisation of active polysaccharides

Liu

Huang

2019

Journal of Enzyme Inhibition and Medicinal Chemistry

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Polysaccharide, as one of the main components of traditional Chinese medicine, has an important antioxidant effect, and its mechanism has been studied by more and more researchers. The preparation methods of Chinese traditional medicine polysaccharides and their derivatives were reviewed. In addition, the advantages and disadvantages of various preparation methods of different Chinese medicine polysaccharides and their derivatives were discussed.

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Characterization of polysaccharides with antioxidant and immunological activities from Rhizoma Acori Tatarinowii

Cited by 61 publications

References 51 publications

Dianxianning improved amyloid β-induced pathological characteristics partially through DAF-2/DAF-16 insulin like pathway in transgenic C. elegans

Dianxianning improved amyloid β-induced pathological characteristics partially through DAF-2/DAF-16 insulin like pathway in transgenic C. elegans

Extraction, Structural Characterization, and Potential Antioxidant Activity of the Polysaccharides from Four Seaweeds

Extraction and derivatisation of active polysaccharides

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