Characterization of Polyvinylchloride by Size Exclusion Chromatography. I. Concentration Dependence of Axial Dispersion Observed in Size Exclusion Chromatography Coupled With Light Scattering

Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Anion-Exchange Chromatography of Bovine Serum Albumin

“…With relatively small molecules, this was reported for phenol and indole on carbon black (Friedrich et al, 1988), for the enantiomeric separation of Tröger's base on a microcrystalline cellulose triacetate (Rearden et al, 1998;Seidel-Morgenstern et al, 1993), and for that of D-and L-phenylalanine anilide on a polymeric imprinted sta-tionary phase . Similar conclusions were reported for the concentration dependence of the intraparticle diffusivity (Al-Duri and McKay, 1992;Gibbs et al, 1991), of the axial dispersion in size exclusion chromatography (Lederer et al, 1990), of the effective diffusivity of a small molecular weight protein in bulk solutions (Gallagher and Woodward, 1989), and of the mutual diffusion coefficient of a macromolecular protein (globular protein ovalbumin) (Marlowe and Jackson, 1990).…”

“…The experimental data derived from breakthrough curves are fitted to the proper model of nonlinear chromatography, and a best value of the rate coefficient is identified. A number of recent studies have concluded to a strong positive dependence of this rate coefficient on the solute concentration (Al‐Duri and McKay, 1992; Friedrich et al, 1988; Gallagher and Woodward, 1989; Gibbs et al, 1991; Guan‐Sajonz et al, 1996; Lederer et al, 1990; Marlowe and Jackson, 1990; Rearden et al, 1998; Sajonz et al, 1997; Sajonz et al, 1998; Seidel‐Morgenstern et al, 1993). With relatively small molecules, this was reported for phenol and indole on carbon black (Friedrich et al .…”

Study of the Mass Transfer Kinetics of BSA on a TSK-GEL DEAE-5PW Anion Exchanger in a Wide Concentration Range

“…These models allow the derivation from experimental breakthrough curves of precise values of the axial dispersion coefficient and of a lumped rate coefficient for the mass transfer kinetics, a constant which includes contributions of all the mass transfer processes. Some investigations indicate a significant dependence of the diffusion (Gallagher and Woodward, 1989;Marlowe and Jackson, 1990) or the axial dispersion coefficient of proteins on their concentration in solution (Lederer et al, 1990). Similar results have also been obtained with smaller molecules (e.g., Tröger's base enantiomers on cellulose microcrystalline triacetate (Seidel-Morgenstern et al, 1993)).…”

A Study of Mass Transfer Kinetics in an Enantiomeric Separation System Using a Polymeric Imprinted Stationary Phase

“…The values of k m,L showed a positive concentration dependence, stronger for L-PA than for D-PA. A similar dependence was observed for k m,L in anion exchange chromatography of bovine serum albumin (BSA) (Guan- Sajonz et al, 1996;Sajonz et al, 1997) and for the mass transfer rate coefficient (k m ) in the enantiomeric separation of Tröger's base on microcrystalline cellulose triacetate (Seidel-Morgenstern et al, 1993;Rearden et al, 1998). Other publications report a concentration dependence of intraparticle diffusivity (Al-Duri and McKay, 1992), tracer diffusivity (Gibbs et al, 1991), axial dispersion (Lederer et al, 1990), effective diffusivity (Gallagher and Woodward, 1989), mutual diffusion coefficient (Marlowe and Jackson, 1990), and surface diffusion coefficients (Friedrich et al, 1988;Kapoor et al, 1989;Miyabe and Guiochon, 2000a). A concentration dependence of the mass transfer rate coefficients (k m and k m,L ) and the diffusivities seems to be a general conclusion.…”