Characterization of porcine uterine estrogen sulfotransferase

Kim, J.G; Vallet, J. L.; Rohrer, G. A.; Christenson, R. K.

doi:10.1016/s0739-7240(02)00172-8

Cited by 10 publications

(7 citation statements)

References 24 publications

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“…Previous studies in pigs have demonstrated that these genes are expressed in the endometrium at significantly higher levels on D12 of pregnancy than on D12 of the estrous cycle (Ka et al, 2000; Kim et al, 2002; Ross et al, 2003b; Seo et al, 2008), indicating the validity of our microarray approach in analyzing gene expression. In addition, many other genes, including ADAMTS17, ADAMTS20, CASP3, CCNA2 , and CYP19 , were also identified as being up-regulated in the endometrium of pregnant pigs on D12 of pregnancy compared with genes on D12 of the estrous cycle.…”

Section: Discussionmentioning

confidence: 71%

Microarray Analysis of Gene Expression in the Uterine Endometrium during the Implantation Period in Pigs

Kim

Seo

Shim

et al. 2012

Asian Australas. J. Anim. Sci

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During embryo implantation in pigs, the uterine endometrium undergoes dramatic morphological and functional changes accompanied with dynamic gene expression. Since the greatest amount of embryonic losses occur during this period, it is essential to understand the expression and function of genes in the uterine endometrium. Although many reports have studied gene expression in the uterine endometrium during the estrous cycle and pregnancy, the pattern of global gene expression in the uterine endometrium in response to the presence of a conceptus (embryo/fetus and associated extraembryonic membranes) has not been completely determined. To better understand the expression of pregnancy-specific genes in the endometrium during the implantation period, we analyzed global gene expression in the endometrium on day (D) 12 and D15 of pregnancy and the estrous cycle using a microarray technique in order to identify differentially expressed endometrial genes between D12 of pregnancy and D12 of the estrous cycle and between D15 of pregnancy and D15 of the estrous cycle. Results showed that the global pattern of gene expression varied with pregnancy status. Among 23,937 genes analyzed, 99 and 213 up-regulated genes and 92 and 231 down-regulated genes were identified as differentially expressed genes (DEGs) in the uterine endometrium on D12 and D15 of pregnancy compared to D12 and D15 of the estrous cycle, respectively. Functional annotation clustering analysis showed that those DEGs included genes involved in immunity, steroidogenesis, cell-to-cell interaction, and tissue remodeling. These findings suggest that the implantation process regulates differential endometrial gene expression to support the establishment of pregnancy in pigs. Further analysis of the genes identified in this study will provide insight into the cellular and molecular bases of the implantation process in pigs.

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Section: Discussionmentioning

confidence: 71%

Microarray Analysis of Gene Expression in the Uterine Endometrium during the Implantation Period in Pigs

Kim

Seo

Shim

et al. 2012

Asian Australas. J. Anim. Sci

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“…Although we did not include incubations with radiolabeled estrogen sulfates to test for sulfatase activity, our findings strongly suggest that the dominant activity is EST, leading to relatively reduced amounts of free E 2 . Recently, a porcine uterine EST has been characterized [27]. Also, EST rather than sulfatase is the more widely expressed enzyme in various human peripheral tissues, including the syncytiotrophoblast of the placenta [28].…”

Section: Discussionmentioning

confidence: 99%

Estrogen Metabolism in the Equine Conceptus and Endometrium During Early Pregnancy in Relation to Estrogen Concentrations in Yolk-Sac Fluid1

Raeside

Christie

Renaud

et al. 2004

Biology of Reproduction

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Because estradiol (E(2)) production by the early equine conceptus is considered crucial to the establishment of pregnancy, the amounts of E(2), estrone (E(1)), and their sulfates (E(2)S, E(1)S) were measured by RIA in yolk-sac fluid of 63 conceptuses collected by transcervical lavage over the period of 11-26 days after ovulation. Amounts increased significantly with age of conceptus, especially for E(1)S. Then, the metabolism of E(2), which may be highly relevant for its action, was examined in the conceptus and endometrium over the period when the conceptus ceases to migrate within the uterus. Eleven conceptuses collected mainly on Days 12, 15, and 18, with endometrial biopsy samples taken immediately thereafter, were used for steroid metabolic studies. Trophoblastic and endometrial tissues were incubated with [(3)H]-labeled E(2) or E(1), and with [(14)C]-E(1) in one experiment. Steroids were recovered from the media by solid-phase extraction (SPE) and eluted separately as unconjugated and conjugated fractions. Conjugation increased from Day 12 for the trophoblast (more so by bilaminar than trilaminar tissues on Day 18) and was much greater for endometrium, with almost all as sulfoconjugates. HPLC profiles of free and sulfate fractions were obtained from a gradient of acetonitrile/water. Interconversion (E(2) right harpoon over left harpoon E(1)) by trophoblast varied with development; it favored E(2) in older conceptuses, more in bilaminar than trilaminar tissues. Some more polar products were also noted, with loss of tritium seen as [(3)H](2)O at SPE, and confirmed by HPLC in a second system with authentic reference steroids. Almost all radioactivity in the endometrium was present as E(2) in both free and sulfate fractions. It was concluded that local metabolism of E(2) is quantitatively significant and may play an important role in the actions of the large amounts of estradiol produced by the early equine conceptus.

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“…SULT1E1 is not present in the susScr3 genome assembly but was previously published as part of QTL candidate gene localization (Kim et al . ). SULT1E1 is a candidate gene for uterine capacity but lies within a region that overlaps a total of 23 QTL, five of which are carcass traits and two of which are reproduction traits (Table S3).…”

Section: Resultsmentioning

confidence: 97%

Discovery of a novel long terminal repeat (LTR2i_SS) in Sus Scrofa

2014

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Long terminal repeat (LTR) retrotransposons are transposable elements flanked by 5'/3' LTRs. They have a structure similar to endogenous retroviruses, but they lack the envelope (env) gene making them non-infectious. Long terminal repeats are motif-rich sequences and can act as bidirectional promoters or enhancers to regulate or inactivate genes by insertion. In this study, we identified a new chimeric LTR subfamily, LTR2i_SS, in the pig genome. This chimeric LTR family appears to be the ancestral form of the previously described LTR2_SS family. LTR2_SS appears to have deleted ~300 bp of un-annotated, ancestral sequence from LTR2i_SS. We identified no functional provirus sequences for either of these LTR types. LTR2i_SS sequences have been exapted into the untranslated regions of two protein-coding gene mRNAs. Both of these genes lie within previously mapped pig quantitative trait loci.

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Characterization of porcine uterine estrogen sulfotransferase

Cited by 10 publications

References 24 publications

Microarray Analysis of Gene Expression in the Uterine Endometrium during the Implantation Period in Pigs

Microarray Analysis of Gene Expression in the Uterine Endometrium during the Implantation Period in Pigs

Estrogen Metabolism in the Equine Conceptus and Endometrium During Early Pregnancy in Relation to Estrogen Concentrations in Yolk-Sac Fluid1

Discovery of a novel long terminal repeat (LTR2i_SS) in Sus Scrofa

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