Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking

Park, Si Nae; Park, Jong Chul; Kim, Hea Ok; Song, Min; Suh, Hwal

doi:10.1016/s0142-9612(01)00235-6

Cited by 479 publications

(347 citation statements)

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“…The modification of hyaluronic acid with use of the carbodiimide (EDC) reaction to create new biopolymers, crosslinking with other biological molecules such as collagen, has been recently and widely investigated for applications such as anti-adhesion membranes 40,57 and biodegradable scaffolds 58,59 , for tissue regeneration, or as timed-release drug-delivery vehicles 60,61 . However, to our knowledge, we are the first to report the use of cd-HA by direct bonding to the tendon surface to improve flexor tendon grafting in vivo.…”

Section: Discussionmentioning

confidence: 99%

Surface Treatment of Flexor Tendon Autografts with Carbodiimide-Derivatized Hyaluronic Acid<sbt aid="1047845">An in Vivo Canine Model</sbt>

Zhao

Sun

Amadio

et al. 2006

J Bone Joint Surg Am

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Background-Clinical and experimental studies have demonstrated that restrictive adhesions and poor digital motion are common complications after extrasynovial tendon grafting in an intrasynovial environment. The purpose of this study was to test the hypothesis that surface modification of an extrasynovial tendon with use of a carbodiimide-derivatized hyaluronic acidgelatin polymer (cd-HA) improves gliding ability and digital function after tendon grafting in a canine model in vivo.

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Section: Discussionmentioning

confidence: 99%

Surface Treatment of Flexor Tendon Autografts with Carbodiimide-Derivatized Hyaluronic Acid<sbt aid="1047845">An in Vivo Canine Model</sbt>

Zhao

Sun

Amadio

et al. 2006

J Bone Joint Surg Am

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“…The effects of T f on pore structure in scaffolds produced from a variety of biomaterials have been investigated in previous studies. [22][23][24][25] However, in these studies, the cooling rate was also varied in conjunction with T f , making it difficult to determine the specific mechanisms behind changes in pore structure. Several studies have investigated the effects of cooling rate on the pore structure of freeze-dried scaffolds.…”

Section: Novel Freeze-drying Methods Applied To Collagen-gag Scaffoldmentioning

confidence: 99%

“…22,25 However, a study by O'Brien et al 13 has shown that cooling rate also affects the uniformity of the pore structure. It was found that a cooling rate of 0.98C=min produced scaffolds with homogeneous pore structure, whereas quicker or slower cooling rates reduced the uniformity of the structure formed.…”

mentioning

confidence: 99%

“…This allowed control of the cooling rate and eliminated the possibility of sample thawing during transport between the freezing and drying apparatus, which were separate devices in many studies. 22,23,25 The use of annealing to alter the pore size of a freeze-dried scaffold is a novel aspect of this study. Annealing has been used previously to encourage crystallization and reduce the drying cycle times of pharmaceuticals, 17,26,27 but has yet to be investigated for its effects on the structure of freeze-dried scaffolds.…”

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confidence: 99%

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Novel Freeze-Drying Methods to Produce a Range of Collagen–Glycosaminoglycan Scaffolds with Tailored Mean Pore Sizes

Haugh

Murphy

O’Brien

2010

Tissue Engineering Part C: Methods

225

196

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• to copy, distribute, display, and perform the work.• to make derivative works. Under the following conditions:• Attribution -You must give the original author credit.• Non-Commercial -You may not use this work for commercial purposes.• The pore structure of three-dimensional scaffolds used in tissue engineering has been shown to significantly influence cellular activity. As the optimal pore size is dependant on the specifics of the tissue engineering application, the ability to alter the pore size over a wide range is essential for a particular scaffold to be suitable for multiple applications. With this in mind, the aim of this study was to develop methodologies to produce a range of collagen-glycosaminoglycan (CG) scaffolds with tailored mean pore sizes. The pore size of CG scaffolds is established during the freeze-drying fabrication process. In this study, freezing temperature was varied (À108C to À708C) and an annealing step was introduced to the process to determine their effects on pore size. Annealing is an additional step in the freeze-drying cycle that involves raising the temperature of the frozen suspension to increase the rate of ice crystal growth. The results show that the pore size of the scaffolds decreased as the freezing temperature was reduced. Additionally, the introduction of an annealing step during freeze-drying was found to result in a significant increase (40%) in pore size. Taken together, these results demonstrate that the methodologies developed in this study can be used to produce a range of CG scaffolds with mean pore sizes from 85 to 325 mm. This is a substantial improvement on the range of pore sizes that were possible to produce previously (96-150 mm). The methods developed in this study provide a basis for the investigation of the effects of pore size on both in vitro and in vivo performance and for the determination of the optimal pore structure for specific tissue engineering applications.

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“…Compared with HA, HA gel is effective in retarding hydrolytic degradation, has less viscosity, and resides in the tissue longer (7 days). 7,34,44 This HA derivative has been shown to be a potent inhibitor of tumor necrosis factor-a (TNFa) and TNFb. 7,8 HA gel is currently used as an adjuvant therapy to prevent postoperative adhesions after laparotomic, laparoscopic, and hysteroscopic abdominopelvic surgical procedures.…”

mentioning

confidence: 99%

Effects of cross-linked high-molecular-weight hyaluronic acid on epidural fibrosis: experimental study

Işık

Taşkapılıoğlu²,

Atalay³

et al. 2015

SPI

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OBJECT Epidural fibrosis is nonphysiological scar formation, usually at the site of neurosurgical access into the spinal canal, in the intimate vicinity of and around the origin of the radicular sheath. The formation of dense fibrous tissue causes lumbar and radicular pain. In addition to radicular symptoms, the formation of scar tissue may cause problems during reoperation. The authors aimed to investigate the effects of cross-linked high-molecular-weight hyaluronic acid (HA), an HA derivative known as HA gel, on the prevention of epidural fibrosis by using histopathological and biochemical parameters. METHODS Fifty-six adult female Sprague-Dawley rats were evaluated. The rats were divided into 4 groups. Rats in the sham group (n = 14) underwent laminectomy and discectomy and received no treatment; rats in the control group (n = 14) underwent laminectomy and discectomy and received 0.9% NaCl treatment in the surgical area; rats in the HA group (n = 14) received HA treatment at the surgical area after laminectomy and discectomy; and rats in the HA gel group (n = 14) underwent laminectomy and discectomy in addition to receiving treatment with cross-linked high-molecular-weight HA in the surgical area. All rats were decapitated after 4 weeks, and the specimens were evaluated histopathologically and biochemically. The results were statistically compared using the Mann-Whitney U-test. RESULTS Compared with the sham and control groups, the HA and HA gel groups showed significantly lower fibroblast cell density and tissue hydroxyproline concentrations (p < 0.05). There was statistically significant lower dural adhesion and foreign-body reaction between the control and HA gel groups (p < 0.05). Granulation tissue and epidural fibrosis were significantly lower in the HA and HA gel groups compared with the sham group (p < 0.05). There were no significant differences in any histopathological parameters or biochemical values between Groups 3 and 4 (p > 0.05). CONCLUSIONS Cross-linked high-molecular-weight HA had positive effects on the prevention of epidural fibrosis and the reduction of fibrotic tissue density. The efficacy of this agent should also be verified in further experimental and clinical studies.

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Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking

Cited by 479 publications

References 23 publications

Surface Treatment of Flexor Tendon Autografts with Carbodiimide-Derivatized Hyaluronic Acid<sbt aid="1047845">An in Vivo Canine Model</sbt>

Surface Treatment of Flexor Tendon Autografts with Carbodiimide-Derivatized Hyaluronic Acid<sbt aid="1047845">An in Vivo Canine Model</sbt>

Novel Freeze-Drying Methods to Produce a Range of Collagen–Glycosaminoglycan Scaffolds with Tailored Mean Pore Sizes

Effects of cross-linked high-molecular-weight hyaluronic acid on epidural fibrosis: experimental study

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