Characterization of Potent Fibrinolytic Enzymes in Earthworm, Lumbricus rubellus

Nakajima, Nobuyoshi; Mihara, Hisashi; Sumi, Hiroyuki

doi:10.1271/bbb.57.1726

Cited by 137 publications

(87 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Lumbricus rubellus who divided them into four groups: F-I-0, F-I-1/2, F-II and F-III-1/2 [11,12] . To date, many other species of earthworm including Eisenia foetida [13] , Neanthes japonica [14] and Pheretima posthuma [15] have been explored for several fibrinolytic enzymes.…”

Section: Coworkers Frommentioning

confidence: 99%

Antitumor Studies of Earthworm Fibrinolytic Enzyme Component A from Eisenia foetida on Breast Cancer Cell Line MCF-7

Liu¹,

Chen²,

Pan³

et al. 2017

pharmaceutical-sciences

View full text Add to dashboard Cite

Liu, et al.: Antitumor Studies of Earthworm Fibrinolytic Enzyme Component A on MCF-7Earthworm fibrinolytic enzyme from Chinese earthworm Eisenia foetida was isolated to investigate its antitumor activity in breast cancer cells. The protein isolated was characterised as earthworm fibrinolytic enzyme using the fibrin plate method. The molecular weight of earthworm fibrinolytic enzyme component was determined to be 25 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis assay using a standard protein ladder. Earthworm fibrinolytic enzyme can markedly inhibit the growth and migration of MCF-7 cells in a dose and time-dependent manner. In addition, MCF-7 cells treated with earthworm fibrinolytic enzyme (40 μg/ml) began to undergo apoptosis after 24 h. Expression of focal adhesion kinase and CD44v6 measured using reverse transcription polymerase chain reaction and Western blot was down-regulated in a concentration-dependent manner (20-80 μg/ml), resulting in the suppression of MCF-7 cells adhesion. The obtained earthworm fibrinolytic enzyme displayed an antitumor effect on MCF-7 cells in vitro, revealing the therapeutic potential of E. foetida.

show abstract

Section: Coworkers Frommentioning

confidence: 99%

Antitumor Studies of Earthworm Fibrinolytic Enzyme Component A from Eisenia foetida on Breast Cancer Cell Line MCF-7

Liu¹,

Chen²,

Pan³

et al. 2017

pharmaceutical-sciences

View full text Add to dashboard Cite

show abstract

“…1,2 The bioactive substances from earthworms so far have not been studied; the scientific evidence of the medicinal use of earthworm species has not been practically proven. 3,4 In recent years, growing earthworms has developed in the neighborhood of Ho Chi Minh City and some provinces of Mekong Delta.…”

Section: Introductionmentioning

confidence: 99%

Survey Process of Autolysis from Earthworm Perionyx Excavates Obtain Protein Solution

Oanh¹

2016

MOJPB

View full text Add to dashboard Cite

This study aimed to survey the process of procuring protein products from earthworm (Perionyx excavates), with the following results: The activity increased with autolysed time up to 10days, but protein content was only highest in the first day and tend to decrease gradually due to the prolonged autolysis which produced undesirable products. With the optimum conditions of hydrolysis process to collect vermiwash, the study was performed by Design Expert 8.0.6 software to optimize at 55°C in 24hours with 13% dilution. The process of hydrolysis created twice the amino acid content and the amino acid values were high after normal vermiwash and centrifugation vermiwash. Vermiwash was preserved by 0.2% potassium sorbate.

show abstract

“…It has long been used as a drug in Chinese medicine under the name of ''Jiryu.'' 11,12) Nakajima et al reported that an earthworm, Lumbricus rubellus, secreted alkaline serine proteases having a potent fibrinolytic activity, 13,14) and that these proteases were also useful biocatalysts. 15,16) Furthermore, they reported that one of the earthworm serine proteases acts on the hydrolysis of triacylglycerols.…”

mentioning

confidence: 99%

Stereoselective Reduction of Carbonyl Compounds Using the Cell-Free Extract of the Earthworm,Lumbricus rubellus, as a Novel Source of Biocatalyst

Ishihara

Nakajima

2006

Bioscience, Biotechnology, and Biochemistry

Self Cite

View full text Add to dashboard Cite

We found the reducing activity toward carbonyl compounds in the cell-free extract of the earthworm, Lumbricus rubellus. The earthworm extract had a reducing activity for keto esters in the presence of NADH or NADPH as a coenzyme. The earthworm extract reduced ethyl 3-methyl-2-oxobutanoate to the corresponding alcohol with a high enantiomeric excess (91%, R-form) at 50 C in the presence of NADH. In particular, ethyl 2-oxoheptanoate was exclusively reduced to the corresponding (R)-hydroxyl ester with a high enantiomeric excess (> 99%).

show abstract

Characterization of Potent Fibrinolytic Enzymes in Earthworm, Lumbricus rubellus

Cited by 137 publications

References 10 publications

Antitumor Studies of Earthworm Fibrinolytic Enzyme Component A from Eisenia foetida on Breast Cancer Cell Line MCF-7

Antitumor Studies of Earthworm Fibrinolytic Enzyme Component A from Eisenia foetida on Breast Cancer Cell Line MCF-7

Survey Process of Autolysis from Earthworm Perionyx Excavates Obtain Protein Solution

Stereoselective Reduction of Carbonyl Compounds Using the Cell-Free Extract of the Earthworm,Lumbricus rubellus, as a Novel Source of Biocatalyst

Contact Info

Product

Resources

About