Characterization of potentially probiotic lactic acid bacteria and bifidobacteria isolated from human colostrum

Abstract

Breast milk is the main source of nutrition for infants; it contains considerable microflora that can be transmitted to the infant endogenously or by breastfeeding, and it plays an important role in the maturation and development of the immune system. In this study, we isolated and identified lactic acid bacteria (LAB) from human colostrum, and screened 2 strains with probiotic potential. The LAB isolated from 40 human colostrum samples belonged to 5 genera: Lactobacillus, Bifidobacterium, Streptococcus, Enterococcus, and Staphylococcus. We also isolated Propionibacterium and Actinomyces. We identified a total of 197 strains of LAB derived from human colostrum based on their morphology and 16S rRNA sequence, among them 8 strains of Bifidobacterium and 10 strains of Lactobacillus, including 3 Bifidobacterium species and 4 Lactobacillus species. The physiological and biochemical characteristics of strains with good probiotic characteristics were evaluated. The tolerances of some of the Bifidobacterium and Lactobacillus strains to gastrointestinal fluid and bile salts were evaluated in vitro, using the probiotic strains Bifidobacterium lactis BB12 and Lactobacillus rhamnosus GG as controls. Among them, B. lactis Probio-M8 and L. rhamnosus Probio-M9 showed survival rates of 97.25 and 78.33% after digestion for 11 h in artificial gastrointestinal juice, and they exhibited growth delays of 0.95 and 1.87 h, respectively, in 0.3% bile salts. These two strains have the potential for application as probiotics and will facilitate functional studies of probiotics in breast milk and the development of human milk-derived probiotics.

Inhibitory Effects of Breast Milk-Derived Lactobacillus rhamnosus Probio-M9 on Colitis-Associated Carcinogenesis by Restoration of the Gut Microbiota in a Mouse Model

“…The Inner Mongolia University of Agriculture isolated and identified lactic acid bacteria from the human colostrum and screened potential probiotic strains. Lactobacillus rhamnosus Probio-M9 [11] can potentially function as a probiotic and improve the intestinal environment by increasing the diversity of the gut flora and regulating metabolic pathways. The effects of Probio-M9 on colorectal tumors have not been assessed yet.…”

Probiotic Potential of Lactic Acid Starter Cultures Isolated from a Traditional Fermented Sorghum-Millet Beverage

“…Tolerance to bile is a prerequisite for colonization and metabolic activity of probiotics in the ileum [ 43 , 50 ]. Bile salts are known to have antimicrobial effects against some microorganisms [ 51 ] and may slow down growth [ 52 ]. They can disrupt the microbial cellular homeostasis as well as dissociate the lipid bilayer and integrity of the cell membrane resulting in cell death [ 12 ].…”

The Preventive Effect of Lactobacillus Fermentum SCHY34 Isolated from Traditional Fermented Yak Yogurt on Lead Acetate-Induced Neurological Damage in SD Rats

“…Therefore, tolerance to gastric juice and intestinal juice determines the number of lactic acid bacteria that pass through the oral cavity and enter the intestine. [25] The stronger the tolerance, the greater the number of viable bacteria that can survive. The hydrophobicity of the surface of lactic acid bacteria reflects the adhesion ability of lactic acid bacteria.…”