Characterization of Probiotic Properties of Antifungal Lactobacillus Strains Isolated from Traditional Fermenting Green Olives

Abouloifa, Houssam; Rokni, Yahya; Bellaouchi, Reda; Ghabbour, Nabil; Karboune, Salwa; Brasca, Milena; Salah, Riadh Ben; Chihib, Nour Eddine; Saalaoui, Ennouamane; Asehraou, Abdeslam

doi:10.1007/s12602-019-09543-8

Cited by 70 publications

(54 citation statements)

References 55 publications

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“…They found that all strains had a high enzymatic activity for leucine arylamidase and valine arylamidase, naphthol-AS-bi-phosphohydrolase, β-galactosidase, and β-glucosidase, while the α-glucosidase activity was lower. Moreover, according to Abouloifa et al [42], Lactobacillus brevis presented the highest β-galactosidase activity, a finding which was also confirmed by the results of this work. Furthermore, the high production of β-glucosidase was previously reported in Lactobacillus strains isolated from green olives [43].…”

Section: Discussionsupporting

confidence: 90%

“…According to Mudryk and Podgórska [41], the level of leucine arylamidase activity is a good measure of the proteolytic activity of bacteria, as it is a peptide bond hydrolyzing enzyme. Abouloifa et al [42] investigated the enzymatic activity of Lactobacillus brevis, Lactobacillus pentosus, and Lactobacillus plantarum isolated from traditional fermenting green olives. They found that all strains had a high enzymatic activity for leucine arylamidase and valine arylamidase, naphthol-AS-bi-phosphohydrolase, β-galactosidase, and β-glucosidase, while the α-glucosidase activity was lower.…”

Section: Discussionmentioning

confidence: 99%

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Influence of the Microalga Chlorella vulgaris on the Growth and Metabolic Activity of Lactobacillus spp. Bacteria

Ścieszka

Klewicka

2020

Foods

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The aim of this study was to evaluate the effect of the algae Chlorella vulgaris on the growth, acidifying activity, proportion of lactic acid isomers, and enzymatic profile of Lactobacillus brevis (ŁOCK 0944, ŁOCK 0980, ŁOCK 0992, and MG451814) isolated from vegetable silages. The results indicated that adding algae at concentrations of 0.1% (w/v) and 1.5% (w/v) to the Lactobacillus spp. growth medium accelerated the growth of bacteria and thus shortened their phase of logarithmic growth. The acidifying activity of the tested Lactobacillus brevis increased with an increased concentration of algae. Lactobacillus spp. cultured in the presence of Chlorella vulgaris showed higher production of l-lactic acid and lower d-lactic acid production. Moreover, the addition of algae changed the enzymatic activity of lactic acid bacteria; for instance, Lactobacillus brevis ŁOCK 0980 demonstrated more enzymatic activity of valine arylamidase, α-galactosidase, and α-glucosidase. Combining Lactobacillus brevis with the algae Chlorella vulgaris allows for the creation of innovative, functional products which confer favorable properties to the final product and open new horizons for the food industry.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Influence of the Microalga Chlorella vulgaris on the Growth and Metabolic Activity of Lactobacillus spp. Bacteria

Ścieszka

Klewicka

2020

Foods

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“…The hemolytic activity assay was adapted from Abouloifa et al [ 9 ]. Initially, suspensions (10 8 CFU.mL −1 ) were spot-inoculated (10 μ L) on 5% blood agar.…”

Section: Methodsmentioning

confidence: 99%

Gardnerella vaginalis and Neisseria gonorrhoeae Are Effectively Inhibited by Lactobacilli with Probiotic Properties Isolated from Brazilian Cupuaçu (Theobroma grandiflorum) Fruit

Selis

Oliveira

Anjos

et al. 2021

BioMed Research International

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In recent years, certain Lactobacillus sp. have emerged in health care as an alternative therapy for various diseases. Based on this, this study is aimed at evaluating in vitro the potential probiotics of five lactobacilli strains isolated from pulp of cupuaçu fruit fermentation against Gardnerella vaginalis and Neisseria gonorrhoeae. Our lactobacilli strains were classified as safe for use in humans, and they were tolerant to heat and pH. Our strains were biofilm producers, while hydrophobicity and autoaggregation varied from 13% to 86% and 13% to 25%, respectively. The coaggregation of lactobacilli used in this study with G. vaginalis and N. gonorrhoeae ranged from 15% to 36% and 32% to 52%, respectively. Antimicrobial activity was present in all tested Lactobacillus strains against both pathogens, and the growth of pathogens in coculture was reduced by the presence of our lactobacilli. Also, all tested lactobacilli reduced the pH of the culture, even in incubation with pathogens after 24 hours. The cell-free culture supernatants (CFCS) of all five lactobacilli demonstrated activity against the two pathogens with a halo presence and CFCS characterization assay together with gas chromatography revealed that lactic acid was the most abundant organic acid in the samples (50% to 62%). Our results demonstrated that the organic acid production profile is strain-specific. This study revealed that cupuaçu is a promising source of microorganisms with probiotic properties against genital pathogens. We demonstrated by in vitro tests that our Lactobacillus strains have probiotic properties. However, the absence of in vivo tests is a limitation of our work due to the need to evaluate the interaction of our lactobacilli with pathogens in the vaginal mucosa. We believe that these findings may be useful in developing a product containing our lactobacilli and their supernatants in order to support with vaginal health.

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“…To test for gelatinase activity, overnight cultures of the tested isolates were spot inoculated on gelatin agar plates and incubated for 48 hrs, then, flooded with 10 mL saturated ammonium sulfate. The presence of a clear zone around the colonies showed gelatinase enzyme production 17 . For the detection of DNase activity, the overnight cultures of isolates were spot inoculated on DNase agar plates and incubated for 48 hrs.…”

Section: Phenotypic Characterization Of Some Virulence Factors Among Selected Probiotic Candidatesmentioning

confidence: 98%

Characterization, in-Vitro Evaluation of Probiotic Potential and Antagonistic Activity of Selected Lactic Acid Bacteria Strains Isolated From Natural Origin Against Some Human Pathogens

Zakaria

Kassem

Far

et al. 2021

Bulletin of Pharmaceutical Sciences. Assiut

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Probiotic bacteria have recently become popular for their health-related beneficial effects. In this study, 19 lactic acid bacteria were isolated from breast milk, dairy products, infant stool, vaginal swabs, fermented beverages, and fermented grapes. These isolates were evaluated for their tolerance to gastrointestinal conditions, hydrophobicity, auto-aggregation, and antimicrobial activity. Five isolates, belonging to Pediococcus spp. and Lactobacillus spp., showed potential probiotic features. These isolates were tested for the presence of virulence enzymes and for susceptibility to various antibiotics. The isolates were found to be nonhemolytic and lacked gelatinase and deoxyribonuclease enzymes, however, some antibiotic resistance genes were detected. To ensure the safety of the used probiotics, cell-free supernatants (CFS) of the promising candidates were prepared and its antimicrobial activity before and after neutralization was assessed against standard strains of Candida albicans, Gram-positive, and Gram-negative bacteria. The neutralization of the CFS significantly diminished the antibacterial and antifungal activities of probiotics. Using the time-kill assay, the combination of CFS of Lactiplantibacillus plantarum with gentamicin and ceftazidime was tested against Staphylococcus aureus and Escherichia coli clinical isolates. A significant reduction in log number of survivors was obtained with gentamicin against E. coli and with ceftazidime against both isolates. The anti-biofilm ability of the CFS of the selected probiotics was tested as well. Neutralized CFS caused 26-52% inhibition of S. aureus isolate biofilm formation compared to the control. In conclusion, the five selected isolates could be considered promising probiotic candidates that can be used as biotherapeutics in case of bacterial infection.

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Characterization of Probiotic Properties of Antifungal Lactobacillus Strains Isolated from Traditional Fermenting Green Olives

Cited by 70 publications

References 55 publications

Influence of the Microalga Chlorella vulgaris on the Growth and Metabolic Activity of Lactobacillus spp. Bacteria

Influence of the Microalga Chlorella vulgaris on the Growth and Metabolic Activity of Lactobacillus spp. Bacteria

Gardnerella vaginalis and Neisseria gonorrhoeae Are Effectively Inhibited by Lactobacilli with Probiotic Properties Isolated from Brazilian Cupuaçu (Theobroma grandiflorum) Fruit

Characterization, in-Vitro Evaluation of Probiotic Potential and Antagonistic Activity of Selected Lactic Acid Bacteria Strains Isolated From Natural Origin Against Some Human Pathogens

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