Characterization of Progenies from Intergeneric Hybridization Between Oryza sativa L. and Porteresia coarctata (Roxb.) Tateoka

Abstract

Porteresia coarctata (Roxb.) Tateoka is an endemic halophyte growing all over the coastal belt of Bangladesh, propagating through rhizomes and setting a few ricelike grains. So exploiting the genetic potential of this wild rice as salt tolerant donor in possible wide crosses with rice (2n = 24) could be useful. We attempted intergeneric hybridization between Oryza sativa L. and P. coarctata. The survival rate of hybrid progenies in embryo culture was low but among them 2 hybrid plants were successfully matured from the intergeneric cross between the cultivated induced tetraploid of rice, Latisail (2n = 4x = 48) and P. coarctata (2n = 48). The hybrid plants could be successfully established in soil and were not like either of the parents in morphology although some of their features were similar to their maternal parent, Latisail (4x). Both of the hybrids were investigated through physiological analysis under salinity stress and molecular analyses with rice specific SSR markers. Molecular analysis of the F 1 DNA with only 3 SSR markers, RM581, RM20224 and RM25271, out of 36 others tested, showed bands specific to both of the parents, while all had common bands with the maternal parent. Dendrogram analysis of the hybrids with the 36 SSR markers, show that P. coarctata forms a different clade and is clearly separated from Latisail and the hybrids. The putative hybrids however made a subgroup with Latisail. These observations could be possibly explained if chromosome loss of the paternal

Paired growth of cultivated and halophytic wild rice under salt stress induces bacterial endophytes and gene expression responses

“…observed any crossover, despite growing Oc side by side with Os over several Attempts at hybridization between the two was also not successful (Islam et al, 2017;Maisha et al, 2023). We have observed that Oc desalinates the water, where it grows (Supplementary Figure 1).…”

“…8 day-old BD67 plants were transferred from the solution to soil in the pots with holes. Oc plant propagules collected from the Teknaf coastal area on the east tip of Bangladesh (Islam et al, 2017) containing roots and rhizomes were allowed to grow in the hydroponic Yoshida solution for 3-4 weeks before transferring to the holed pots. The pots of Os and Oc were placed alternately in the bowl described above (paired) filled with water up to the brim of the pots (3 each in one).…”

“…Therefore, it likely reduces that salinity of the soil or water that it is proximal to and in this process of detoxification, may help a less tolerant plant to grow by its side. The potential danger of gene flow from the wild Oc to cultivated Os to make the latter weedy (Roma-Burgos et al, 2021), was deemed minimal because forced hybridization between the two did not result in successful outcome (Islam et al, 2017;Maisha et al, 2023).…”

Intergeneric hybridization of marguerite (<i>Argyranthemum frutescens</i> (L.) Sch. Bip.) and Roman chamomile (<i>Chamaemelum nobile</i> (L.) All.) using ovule culture and confirmation of hybridity

“…Apart from the flow cytometry analysis, DNA marker determination has been widely reported as a method to confirm hybridization (Ballesfin et al 2018;Islam et al 2017;Lou et al 2017;Premjet et al 2019;Punjansing et al 2021). In the intergeneric hybrids of Argyranthemum, Random Amplified Polymorphic DNA markers revealed hybridity with I. carinata and G. coronaria (Morikawa et al 2014;Ueda and Yamada 2005), and CAPS marker and sequence-characterized amplified region markers have been reported to discriminate between hybrids of R. gayanum (Muto et al 2020a(Muto et al , 2020b.…”

C4 photosynthesis with Kranz anatomy evolved in the Oryza coarctata Roxb

“…Several salt stress responsive genes from this plant had been characterized (Sengupta et al, 2009;Kizhakkedath et al, 2015). Moreover, attempts were made for introgression of salt stress responsive genes from O. coarctata into rice cultivar (Jena, 1994;Islam et al, 2017) through conventional crossing method. Although, O. coarctata is an obligate halophytic grass but no attempt was made to study its mode of photosynthesis.…”