Characterization of prostanoid receptors mediating actions of the isoprostanes, 8‐iso‐PGE<sub>2</sub> and 8‐iso‐PGF<sub>2α</sub>, in some isolated smooth muscle preparations

Sametz, W.; Hennerbichler, Simone; Glaser, Sonja; Wintersteiger, Reinhold; Juan, H.

doi:10.1038/sj.bjp.0703522

Cited by 60 publications

(53 citation statements)

References 33 publications

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“…Other reports have suggested that, in the presence of oxidative damage, PG could be converted into products of oxidation, such as 8-iso-PGF 2a (Natale et al 2004), that induce vasoconstriction and platelet aggregation that might enhance ulcerogenic actions of necrotizing agent and promote tissue oxidation (Sametz et al 2000). The effect of fluoxetine on gastric acid secretion and subsequently higher gastric pH compared to values in the untreated ulcer counterparts may be secondary to its effect on NO and COX enzymes.…”

Section: Discussionmentioning

confidence: 97%

Serotonin and histamine mediate gastroprotective effect of fluoxetine against experimentally-induced ulcers in rats

Sokar

Elsayad

Ali

2016

Journal of Immunotoxicology

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Research in the treatment of gastric ulcer has involved the investigation of new alternatives, such as anti-depressant drugs. The present study was designed to investigate the gastroprotective effects of fluoxetine against indomethacin and alcohol induced gastric ulcers in rats and the potential mechanisms of that effect. Fluoxetine (20 mg/kg) was administered IP for 14 days. For comparative purposes, other rats were treated with ranitidine (30 mg/kg). Thereafter, after 24 h of fasting, INDO (100 mg/kg) or absolute alcohol (5 ml/kg) was administered to all rats (saline was administered to naïve controls) and rats in each group were sacrificed 5 h (for INDO rats) or 1 h (for alcohol rats) later. Macroscopic examination revealed that both fluoxetine and ranitidine decreased ulcer scores in variable ratios, which was supported by microscopic histopathological examination. Biochemical analysis of fluoxetine-or ranitidine-pre-treated host tissues demonstrated reductions in tumor necrosis factor (TNF)-and myeloperoxidase (MPO) levels and concomitant increases in gastric pH, nitric oxide (NO) and reduced glutathione (GSH) contents. Fluoxetine, more than ranitidine, also resulted in serotonin and histamine levels nearest to control values. Moreover, immuno-histochemical analysis showed that fluoxetine markedly enhanced expression of cyclooxygenases COX-1 and COX-2 in both models; in comparison, ranitidine did not affect COX-1 expression in either ulcer model but caused moderate increases in COX-2 expression in INDOinduced hosts and high expression in alcohol-induced hosts. The results here indicated fluoxetine exhibited better gastroprotective effects than ranitidine and this could be due to anti-secretory, anti-oxidant, anti-inflammatory and anti-histaminic effects of the drug, as well as a stabilization of gastric serotonin levels. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 97%

Serotonin and histamine mediate gastroprotective effect of fluoxetine against experimentally-induced ulcers in rats

Sokar

Elsayad

Ali

2016

Journal of Immunotoxicology

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“…Since it has been established that stimulation of prostanoid receptors caused contraction in the guinea pig ileum (34), prostaglandin-like compounds may be involved in [6]-gingerol-induced contraction. However, [6]-gingerol is reported to be an inhibitor of prostaglandin synthetase and 5-lipoxygenase in vitro (35,36).…”

Section: Involvement Of Nk 1 Receptors and Prostaglandins Formation Omentioning

confidence: 99%

Modifications of Capsaicin-Sensitive Neurons in Isolated Guinea Pig Ileum by [6]-Gingerol and Lafutidine

et al. 2003

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Abstract. A segment of guinea pig ileum was used to confirm the hypothesis that [6]-gingerol and lafutidine interact with capsaicin-sensitive neurons. Addition of 30 and 100 m M [6]-gingerol (a pungent constituent of ginger) induced contraction of the ileum immediately. Like capsaicin, [6]-gingerol-induced contraction was inhibited by antagonists of the vanilloid receptor (capsazepine and ruthenium red), tetrodotoxin, and atropine. Treatment with [6]-gingerol up to 0.3 m M, which alone had no effect, enhanced 3 m M capsaicin-induced contraction, but greater than 3 mM [6]-gingerol significantly inhibited capsaicin-induced contraction. Treatment with lafutidine (a new type of antagonist of the histamine H 2 receptor), which was suggested to interact with capsaicin-sensitive neurons in vivo, also showed both stimulatory and inhibitory effects on capsaicin-induced contraction depending on the concentrations. Lafutidine alone had no effect. The enhanced contraction induced by capsaicin in the [6]-gingerol-or lafutidine-treated ileum was also inhibited by antagonists of the vanilloid receptor, tetrodotoxin, and atropine. Capsaicin and [6]-gingerol, but not lafutidine, at 30 m M stimulated [ 3 H]choline release from the prelabeled slices of the ileum. These findings suggest that [6]-gingerol and lafutidine act on capsaicin-sensitive cholinergic neurons and modulate the contraction in isolated guinea pig ileum.

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“…Isoprostane 8-iso-PGF 2␣ appears to act through the thromboxane-selective TP prostanoid receptor to induce vasoconstriction (13,17) and bronchoconstriction (25). Some studies indicate that 8-iso-PGE 2 may act similarly on the TP receptor (12,(22)(23)(24)42), whereas others indicate the involvement of FP and EP prostanoid receptors in mediating the excitatory activity of 8-iso-PGE 2 (40,44). Inhibitory activity is also observed for the isoprostanes (8,22), with 8-iso-PGE 2 seemingly acting at the inhibitory EP receptors to induce airway relaxation (8).…”

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confidence: 99%

Augmentation of bovine airway smooth muscle responsiveness to carbachol, KCl, and histamine by the isoprostane 8-iso-PGE₂

Catalli¹,

Janssen²

2004

American Journal of Physiology-Lung Cellular and Molecular Phys

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Catalli, Adriana, and Luke J. Janssen. Augmentation of bovine airway smooth muscle responsiveness to carbachol, KCl, and histamine by the isoprostane 8-iso-PGE 2. Am J Physiol Lung Cell Mol Physiol 287: L1035-L1041, 2004. First published July 16, 2004 doi:10.1152/ajplung.00138.2004Isoprostanes are generated during periods of oxidative stress, which characterize diseases such as asthma and cystic fibrosis. They also elicit functional responses and may therefore contribute to the pathology of these diseases. We set out to examine the effects of isoprostanes on airway responsiveness to cholinergic stimulation. Muscle bath techniques were employed using isolated bovine tracheal smooth muscle. 8-Isoprostaglandin E 2 (8-iso-PGE 2) increased tone directly on its own, although the magnitude of this response, even at the highest concentration tested, was only a fraction of that evoked by KCl or carbachol. More importantly, though, pretreatment of the tissues with 8-iso-PGE 2 (10 M) markedly augmented responses to submaximal and even subthreshold concentrations of KCl, carbachol, or histamine, whereas maximal responses to these agents were unaffected by the isoprostane. The augmentative effect on cholinergic responsiveness was mimicked by PGE 2 (0.1 M) and by the FP agonists PGF2 (0.1 M) and fluprostenol (0.1 M), but not by the EP3 agonist sulprostone (0.1 M) or the TP agonist U-46619 (0.1 M). Antagonists of EP1 receptors (AH-6809 and SC-19920, 10 M) and TP receptors (ICI-192605, 1 M) had no effect on 8-iso-PGE2-induced augmentation of cholinergic responsiveness. We conclude that 8-iso-PGE2 induces nonspecific airway smooth muscle hyperresponsiveness through a non-TP non-EP prostanoid receptor. prostanoid receptors; contraction; asthma; oxidative stress; 8-isoprostaglandin E2 ISOPROSTANES ARE A LARGE GROUP of free radical-generated, prostaglandin-like molecules that have proven useful as markers of oxidative stress. Only recently have researchers begun to recognize these molecules as having their own potent biological activity. Most research to date has focused solely on the excitatory actions of isoprostane 8-iso-PGF 2␣ , which is responsible for such physiological responses as vasoconstriction, bronchoconstriction (22,25,27), and modulation of platelet aggregation (28,38).Isoprostanes are detected in normal human plasma, urine, bronchoalveolar lavage fluid (33-35), and exhaled breath condensates (31). More importantly, their concentrations are significantly elevated during periods of oxidative stress, which characterizes such diseases as asthma (2,3,31,45), chronic obstructive pulmonary disorder (30, 37), interstitial lung disease (29), and cystic fibrosis (9,11,32) and as such may contribute to the pathology underlying these conditions.There are a number of reports indicating that isoprostanes exert their biological effects through classic prostanoid receptors. This is not surprising considering the strong structural similarities between isoprostanes and prostaglandins; both groups of molecules possess a cyclopentane ...

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Characterization of prostanoid receptors mediating actions of the isoprostanes, 8‐iso‐PGE₂ and 8‐iso‐PGF_2α, in some isolated smooth muscle preparations

Cited by 60 publications

References 33 publications

Serotonin and histamine mediate gastroprotective effect of fluoxetine against experimentally-induced ulcers in rats

Serotonin and histamine mediate gastroprotective effect of fluoxetine against experimentally-induced ulcers in rats

Modifications of Capsaicin-Sensitive Neurons in Isolated Guinea Pig Ileum by [6]-Gingerol and Lafutidine

Augmentation of bovine airway smooth muscle responsiveness to carbachol, KCl, and histamine by the isoprostane 8-iso-PGE₂

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Characterization of prostanoid receptors mediating actions of the isoprostanes, 8‐iso‐PGE2 and 8‐iso‐PGF2α, in some isolated smooth muscle preparations

Cited by 60 publications

References 33 publications

Serotonin and histamine mediate gastroprotective effect of fluoxetine against experimentally-induced ulcers in rats

Serotonin and histamine mediate gastroprotective effect of fluoxetine against experimentally-induced ulcers in rats

Modifications of Capsaicin-Sensitive Neurons in Isolated Guinea Pig Ileum by [6]-Gingerol and Lafutidine

Augmentation of bovine airway smooth muscle responsiveness to carbachol, KCl, and histamine by the isoprostane 8-iso-PGE2

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Product

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Characterization of prostanoid receptors mediating actions of the isoprostanes, 8‐iso‐PGE₂ and 8‐iso‐PGF_2α, in some isolated smooth muscle preparations

Augmentation of bovine airway smooth muscle responsiveness to carbachol, KCl, and histamine by the isoprostane 8-iso-PGE₂