Characterization of protease-producing bacteria from garden soil and antagonistic activity against pathogenic bacteria

Afrin, Sadia; Tamanna, Tasnim; Shahajadi, Ummey Fatema; Bhowmik, Banasree; Jui, Afsana Habib; Miah, Md. Abdus Satter; Bhuiyan, Mohammad Nazrul Islam

doi:10.1016/j.microb.2024.100123

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…Optimization of fermentation conditions, scalability of production processes and cost-effectiveness are critical factors that need to be considered for industrial-scale rhamnolipid production. Furthermore, there is a need for additional research to investigate the variety of bacteria capable of producing rhamnolipids or other secondary metabolites across diverse environmental settings [ 39 41 ]. Additionally, efforts should be directed towards the development of microbial strains engineered to exhibit improved biosurfactant production capacities and other desirable properties.…”

Section: Resultsmentioning

confidence: 99%

Exploration and characterization of a newly isolated bacterium, Enterobacter quasihormaechei strain BDIFST24001, capable of producing rhamnolipid biosurfactant for oil remediation

Jui,

Bhuiyan,

Bhowmik

et al. 2024

Access Microbiology

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Biosurfactants are naturally occurring compounds synthesized by micro-organisms that increasingly attract attention due to both their living area and application in various industries. In this study, we explore and characterize a novel bacterium, Enterobacter quasihormaechei strain BDIFST24001, isolated for its ability to produce rhamnolipid biosurfactants, with the aim of facilitating oil remediation processes. The isolation of this bacterium was carried out using Luria-Bertani (LB) broth media from environmental samples collected from oil-contaminated sites in Dhaka City. Screening tests, including the oil spreading method and drop collapse assay, were conducted to identify potential biosurfactant-producing strains, leading to the selection of E. quasihormaechei strain BDIFST24001 based on its favourable performance. Subsequent molecular identification revealed a high similarity of the strain’s 16S rRNA gene to E. quasihormaechei, which was corroborated through phylogenetic analysis. Further analysis of the biosurfactant produced by this strain indicated its rhamnolipid nature, as confirmed by FT-IR spectroscopy. The rhamnolipids exhibited promising surface-active properties, including a significant reduction in surface tension and emulsification activity, as evidenced by surface tension measurements and emulsification index assays. Optimization studies revealed that the optimal conditions for rhamnolipid production by E. quasihormaechei strain BDIFST24001 were a temperature of 37 °C, pH 10.0 and salinity of 4 %. The rhamnolipids produced by this strain demonstrated effective oil remediation capabilities, as observed through controlled experiments using petrol oil. The rhamnolipids effectively reduced the surface tension of the oil–water interface, facilitating the dispersion and emulsification of the oil phase in water. Overall, our findings highlight the potential of E. quasihormaechei strain BDIFST24001 as a promising candidate for biosurfactant-mediated oil spill cleanup and environmental remediation efforts.

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Section: Resultsmentioning

confidence: 99%

Exploration and characterization of a newly isolated bacterium, Enterobacter quasihormaechei strain BDIFST24001, capable of producing rhamnolipid biosurfactant for oil remediation

Jui,

Bhuiyan,

Bhowmik

et al. 2024

Access Microbiology

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Exploring the purification, characterization, and industrial applications of Exopolysaccharide (EPS) from Bacillus amyloliquefaciens strain BDIFST240014

Bhowmik,

Afrin,

Jui

et al. 2024

Mol Biol Rep

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Screening, Identification, and Optimization of Enzyme Production for Bacteria with High Efficiency of Bovine Blood Protein Degradation from Slaughterhouses

Ma,

Li,

Abdul

et al. 2024

Water

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Blood waste originating from slaughterhouse activities in China is considered to be massive, and improper handling may cause epidemic diseases and environmental pollution. This research aimed to obtain a potential bacterial strain for blood protein degradation by isolating indigenous bacteria from slaughterhouses. Isolation was carried out by using dilution plate coating, while blood agar and casein plates were used to screen potential strains. Morphological, physiological, and biochemical characterizations, as well as 16S rRNA sequencing, were performed to identify the selected strain. The optimization of enzyme production was performed by using the Plackett–Burman test and response surface methodology. A strain coded NwMCC01910137 was isolated and screened to effectively degrade bovine blood protein and was identified as Bacillus subtilis. The optimum culture conditions for enzyme production included a fermentation temperature of 37.4 °C, an initial pH of 7.7, a soybean meal powder addition amount of 3.00% (w/v), a glucose level of 3.8% (w/v), a NaCl level of 0.3 g/L, a phosphate concentration of 2.5 g/L, an inoculum size of 2.5 g/L (4%), and a fermentation time of 48 h. Under the optimum condition, the strain showed enzyme activity of 166.83 U/mL. Hence, the isolated B. subtilis showed good activity in bovine blood protein degradation and has good application potential for slaughterhouse waste processing.

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Characterization of protease-producing bacteria from garden soil and antagonistic activity against pathogenic bacteria

Cited by 3 publications

References 30 publications

Exploration and characterization of a newly isolated bacterium, Enterobacter quasihormaechei strain BDIFST24001, capable of producing rhamnolipid biosurfactant for oil remediation

Exploration and characterization of a newly isolated bacterium, Enterobacter quasihormaechei strain BDIFST24001, capable of producing rhamnolipid biosurfactant for oil remediation

Exploring the purification, characterization, and industrial applications of Exopolysaccharide (EPS) from Bacillus amyloliquefaciens strain BDIFST240014

Screening, Identification, and Optimization of Enzyme Production for Bacteria with High Efficiency of Bovine Blood Protein Degradation from Slaughterhouses

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