Characterization of protein synthesis by isolated rice mitochondria

Dai, Hwa; Lo, Yih‐Shan; Charn, Chyr-Guan; Ruddat, Manfred; Chiang, Kwen‐Sheng

doi:10.1007/bf00222094

Cited by 5 publications

(1 citation statement)

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“…Not only RNA abundance, but also protein abundance varies in response to growth and environmental variations in mitochondria as it was documented for maize [ 37 , 38 ], rice [ 39 , 40 ], sugar beet [ 41 ], Nicotiana sylvestris [ 42 ], petunia [ 43 ] and Arabidopsis thaliana [ 34 ]. In these studies, increased mitochondrial protein synthesis was observed in leaves, flowers, shoots [ 41 , 43 ], and seeds [ 34 ], while a decrease was reported in roots [ 41 ], after heat [ 39 , 44 ], cold [ 40 ], chloramphenicol, erythromycin, cycloheximide [ 39 ] and methomyl treatments [ 45 ]. Due to differences in the stability or life time of different proteins, the reported variations in protein abundance cannot directly be used as a measure of translational activity.…”

Section: Introductionmentioning

confidence: 99%

Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues

et al. 2017

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BackgroundTo investigate translational regulation of gene expression in plant mitochondria, a mitochondrial polysome isolation protocol was established for tobacco to investigate polysomal mRNA loading as a proxy for translational activity. Furthermore, we developed an oligonucleotide based microarray platform to determine the level of Nicotiana tabacum and Arabidopsis thaliana mitochondrial mRNA.ResultsMicroarray analysis of free and polysomal mRNAs was used to characterize differences in the levels of free transcripts and ribosome-bound mRNAs in various organs of tobacco plants. We have observed higher mitochondrial transcript levels in young leaves, flowers and floral buds as compared to fully expanded leaves and roots. A similar pattern of abundance was observed for ribosome-bound mitochondrial mRNAs in these tissues. However, the accumulation of the mitochondrial protein COX2 was found to be inversely related to that of its ribosome-bound mRNA.ConclusionsOur results indicate that the association of mitochondrial mRNAs to ribosomes is largely determined by the total transcript level of a gene. However, at least for Cox2, we demonstrated that the level of ribosome-bound mRNA is not reflected by the amount of COX2 protein.Electronic supplementary materialThe online version of this article (10.1186/s13007-017-0257-4) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues

et al. 2017

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Variation in protein and RNA synthesis activity in isolated mitochondria of the developing rice (Oryza sativa L.) panicle

Dai

Wang

et al. 1995

Theoret. Appl. Genetics

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We have studied variation in mitochondrial protein and RNA synthesis during the development of a specialized rice (Oryza sativa L.) reproductive organ in a bacteria-free environment. Mitochondria were prepared from the maturing panicle during microsporogenesis when meiosis occurred and from etiolated seedlings at two growth stages. We found (1) that there was no discernible qualitative difference among the polypeptides synthesized by these three mitochondrial samples; (2) that the quantity of proteins synthesized by panicle mitochondria was approximately 3 times that of the seedling mitochondria, while the two seedling samples exhibited only a minor quantitative difference; (3) that panicle and seedling mitochondria samples synthesized qualitatively the same RNA but at distinctly different rates and that more RNA products were synthesized by panicle than by seedling mitochondria. These results, taken together, suggest that either the regulation of mitochondrial transcription and translation or the copy number of mitochondrial DNA per mitochondrion change discretely in the developing panicle and consequently that the level of mitochondrial gene expression increases considerably during the development of the reproductive structure in rice.

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Induction of cell death in Arabidopsis by superoxide in combination with salicylic acid or with protein synthesis inhibitors

Mazel

Levine

2001

Free Radical Biology and Medicine

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Characterization of protein synthesis by isolated rice mitochondria

Cited by 5 publications

References 20 publications

Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues

Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues

Variation in protein and RNA synthesis activity in isolated mitochondria of the developing rice (Oryza sativa L.) panicle

Induction of cell death in Arabidopsis by superoxide in combination with salicylic acid or with protein synthesis inhibitors

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