Characterization of Prunus domestica L. in vitro regeneration via hypocotyls

Tian, Lining; Sibbald, Susan; Subramanian, Jayasankar; Svircev, A. M.

doi:10.1016/j.scienta.2007.01.026

Cited by 18 publications

(15 citation statements)

References 13 publications

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“…Genetic transformation in European plum (Prunus domestica L.) has been established through regeneration of the cotyledon and hypocotyl sections of mature embryos (Mante et al, 1989;Scorza et al, 1994), immature embryos and embryonic axes (Tian et al, 2007a;Srinivasan et al, 2012;Petri et al, 2012: Wang et al, 2013. Medium supplemented with thidiazuron (TDZ) 7.5 μM in combination with indole-3-butyric acid (IBA) 0.25 μM has been used for shoot regeneration, with a transformation effi ciency of 1.2% (Scorza et al, 1994;González-Padilla et al, 2003).…”

Section: Genetic Transformationmentioning

confidence: 99%

“…Shoots were induced from hypocotyl segments of mature seeds on medium containing TDZ, 6-benzylaminopurine (BA) and kinetin. TDZ medium was the most eff ective for shoot regeneration, with an effi ciency of 30% of explants forming shoots; the shoot induction response was genotype-dependent (Tian et al, 2007a). Urtubia et al (2008), using a modified protocol for regeneration of European plum (Scorza et al, 1994), obtained 11% and 19% shoot regeneration from hypocotyl slices in the Japanese plum cultivars "Angeleno" and "Larry Anne", respectively.…”

Section: Genetic Transformationmentioning

confidence: 99%

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Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

et al. 2013

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This review is an overview of traditional and modern breeding methodologies being used to develop new Prunus cultivars (stone fruits) with major emphasis on peach, sweet cherry and Japanese plum. To this end, common breeding tools used to produce seedlings, including in vitro culture tools, are discussed. Additionally, the mechanisms of inheritance of many important agronomical traits are described. Recent advances in stone fruit transcriptomics and genomic resources are providing an understanding of the molecular basis of phenotypic variability as well as the identifi cation of allelic variants and molecular markers. These have potential applications for understanding the genetic diversity of the Prunus species, molecular marker-assisted selection and transgenesis. Simple Sequence Repeat (SSR) and Single Nucleotide Polymorphism (SNPs) molecular markers are described as useful tools to describe genetic diversity in peach, sweet cherry and Japanese plum. Additionally, the recently sequenced peach genome and the public release of the sweet cherry genome are discussed in terms of their applicability to breeding programs.

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Section: Genetic Transformationmentioning

confidence: 99%

Section: Genetic Transformationmentioning

confidence: 99%

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

et al. 2013

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“…Particularly, in the common plum (Prunus domestica L.), a commercially important stone fruit tree, the rooting efficiency is low, despite the attempts directed towards higher effectiveness of the process (choice of the auxin, root induction procedure, one-or multi-step culturing, treatment duration etc.) (Gonzales Padilla et al 2003;Tian et al 2007). Despite numerous studies on regeneration potential of the common plum, the process of adventitious rhizogenesis itself has not been investigated extensively in this important fruit tree.…”

Section: Introductionmentioning

confidence: 99%

Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Wiszniewska

Nowak

Kołton

et al. 2016

Plant Cell Tiss Organ Cult

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The study aimed at evaluating the response of common plum (Prunus domestica L.) microshoots during in vitro rooting in the presence of two phytoactive medium supplements, i.e. a dialyzate of pineapple pulp and a conditioned medium containing green algae Desmodesmus subspicatus exudates. Rooting efficiency was evaluated after 4 weeks of culture. During the root induction phase the content of phenolic compounds in shoot bases was determined and anatomical studies were conducted. Medium supplements were analyzed for the content of carbohydrates and phenolic acids. Both supplements were efficient in rooting induction of shoots of a difficult-to-root cultivar 'Węgierka Zwykła'. Medium supplementation allowed a significant reduction in the exogenous auxin content required for rooting. The highest rooting efficiencies on supplemented media were 28.9 and 27.8 %, in comparison with 33.3 % obtained in the control medium with doubled concentration of exogenous auxins. In the easy-to-root plum cultivar 'Węgierka Dąbrowicka' the rooting rate was slightly reduced in the presence of pineapple dialyzate, while in the presence of algal conditioned medium the rooting rate decreased substantially compared with the nonsupplemented medium. Approximately 30 % of 'Węgierka Dąbrowicka' shoots rooted on supplemented auxin-free media. The content of phenolic compounds accumulated in shoot bases during the root induction stage reflected the differences in rooting ability between both plum cultivars, indicating potential stressful conditions of the culture generated by the presence of phytoactive natural supplements. Anatomical study allowed to recognize the mode of dedifferentiation leading to adventitious rhizogenesis in the common plum. The results are discussed in relation to the composition of medium supplements and their potential root-promoting activity.

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“…communication). Our previous studies indicate that these plums have low response to in vitro regeneration (Tian et al 2006) and the genetic transformation efficiency is also low (Tian et al 2009). Plum fruits, two weeks prior to maturation, were collected from plum trees in Vineland, Ontario, Canada.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Indeed, European plum genetic transformation has only been successful in a few laboratories. Tian et al (2006) evaluated in vitro regeneration of European plum germplasms and varieties adapted to high latitude and developed genetic transformation technology for these types of plum plants via hopcotyl regeneration (Tian et al 2009). Nevertheless, at this time, wide and practical use of plum genetic transformation technology in many other laboratories and studies is still not feasible and is difficult.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Factors Affecting European Plum (Prunus domestica L.) Genetic Transformation

Yuan¹,

Ali²,

Meerja³

et al. 2011

Genetic Transformation

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Characterization of Prunus domestica L. in vitro regeneration via hypocotyls

Cited by 18 publications

References 13 publications

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Evaluation of Factors Affecting European Plum (Prunus domestica L.) Genetic Transformation

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