Characterization of Pseudoperonospora cubensis isolates from Europe and Asia using ISSR and SRAP molecular markers

Polat, İlknur; Baysal, Ömür; Mercati, Francesco; Kitner, Miloslav; Cohen, Yigal; Lebeda, A.; Carimi, Francesco

doi:10.1007/s10658-014-0420-y

Cited by 25 publications

(20 citation statements)

References 47 publications

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“…A similar pattern was also obtained from a comparison of Czech, Turkish and Israeli P. cubensis isolates by DNA fingerprinting (Polat et al ., ), with a subset of 15 out of 30 Czech isolates (sampled throughout 1996–2003) also being used in the present study. Turkish isolates were identical in their DNA fingerprints, and exhibited similarity to the Czech isolates (all from cucumber), while the isolates from Israel were distinct from the Czech and Turkish isolates (Polat et al ., ). Because all Czech isolates analysed in both previous studies (Quesada‐Ocampo et al ., ; Polat et al ., ) fell into Clade II, and they exhibit similar genetic profiles to the Turkish isolates, this points to a common origin of the Turkish and Czech isolates.…”

Section: Discussionmentioning

confidence: 99%

“…() with a slightly different cluster composition, compared to isolates from Israel. A similar pattern was also obtained from a comparison of Czech, Turkish and Israeli P. cubensis isolates by DNA fingerprinting (Polat et al ., ), with a subset of 15 out of 30 Czech isolates (sampled throughout 1996–2003) also being used in the present study. Turkish isolates were identical in their DNA fingerprints, and exhibited similarity to the Czech isolates (all from cucumber), while the isolates from Israel were distinct from the Czech and Turkish isolates (Polat et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…Turkish isolates were identical in their DNA fingerprints, and exhibited similarity to the Czech isolates (all from cucumber), while the isolates from Israel were distinct from the Czech and Turkish isolates (Polat et al ., ). Because all Czech isolates analysed in both previous studies (Quesada‐Ocampo et al ., ; Polat et al ., ) fell into Clade II, and they exhibit similar genetic profiles to the Turkish isolates, this points to a common origin of the Turkish and Czech isolates.…”

Section: Discussionmentioning

confidence: 99%

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Coincidence of virulence shifts and population genetic changes of Pseudoperonospora cubensis in the Czech Republic

et al. 2015

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Pseudoperonospora cubensis is an oomycete pathogen causing downy mildew disease on a variety of Cucurbitaceae, and has recently re‐emerged as a destructive disease on crops in this family, mainly on cucumber and squash. Multilocus sequence analysis (MLSA) of four mitochondrial and two nuclear DNA regions was used to detect changes in the genetic structure of P. cubensis populations occurring in the Czech Republic that might be associated with recently reported shifts in virulence. The analysed sample set contains 67 P. cubensis isolates collected from 1995 to 2012 in the Czech Republic and some other European countries. Sequence analyses revealed differences and changes in the genetic backgrounds of P. cubensis isolates. While all isolates sampled before 2009 exhibited the genotype of the subspecies of Clade II and were collected from cucumber, all samples collected from other hosts belonged to Clade I (P. cubensis sensu stricto) or were sampled from 2009 onwards. In addition, 67·16% of all post‐2009 isolates from Clade II had two heterozygous positions in their nrITS sequence, which suggests sexual reproduction and/or a mutational origin. Thus, the results indicate that, apart from the rise in prevalence of Clade I, the change in the genetic structure of P. cubensis populations may be linked with a hybridization or, less likely, a mutation event that rendered strains able to infect a broader spectrum of host species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Coincidence of virulence shifts and population genetic changes of Pseudoperonospora cubensis in the Czech Republic

et al. 2015

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“…These results indicate that different populations of P. cubensis exist in close proximity in Central Asia. Previous studies examining the genetic structure of P. cubensis (Sarris et al ., ; Quesada‐Ocampo et al ., ; Polat et al ., ) have shown that the high genetic differentiation within pathogen populations in local and adjacent regions may drive pathogenic variability within those regions.…”

Section: Discussionmentioning

confidence: 99%

Pseudoperonospora cubensis virulence and pathotype structure in Kazakhstan

Rsaliyev¹,

Amirkhanova²,

Rametov³

et al. 2018

Plant Pathology

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Severe epidemics of cucurbit downy mildew caused by Pseudoperonospora cubensis have been observed in recent years in major cucumber‐producing regions in Kazakhstan. However, information on the pathogenic variability of P. cubensis that could facilitate screening of candidate cucumber varieties and hybrids prior to deployment for commercial production is currently not available. A total of 112 isolates were collected from 2014 to 2016 during surveys in three major cucumber‐producing regions of Kazakhstan. Single lesion isolates were tested for their compatibility with a differential host set composed of 12 genotypes. Most isolates were compatible with Cucumis sativus and C. melo subsp. melo, while none were compatible with Citrullus lanatus. Isolates differed primarily by their virulence to C. pepo subsp. pepo and Benincasa hispida. Based on the patterns of compatibility with the differential host set, 29 pathotypes were identified. Of these, 18 were associated with a specific production region, six occurred in two regions and five were present in all three regions. The number of virulence factors (VF) ranged from 2 to 10, with 65% of the pathotypes having eight or more VFs. Indices of diversity showed that virulence was very diverse within the pathogen populations. Clustering of pathotypes based on similarity in virulence showed the existence of two main clusters. The high pathogenic variability observed in this study suggests that newly developed cucumber varieties and hybrids with resistance to cucurbit downy mildew need to be evaluated against a wide range of pathotypes prior to their deployment for commercial production in Kazakhstan.

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“…An extensive characterization of pathogenic variation has also shown a shift toward a higher number of virulence factors over time (58). Processes that drive variation are not well understood but may reflect local adaptation due to climatic factors that influence effective population size, heterogeneity of hosts at multiple spatial scales, and migration via long-distance dispersal (58,81,109). Populations of P. cubensis are dominated by relatively few genetic clusters, many of which are shared among separate production regions, and states such as North Carolina and Florida have highly diverse pathogen populations (84).…”

Section: Pathogenic Variationmentioning

confidence: 99%

Epidemiology and Population Biology of Pseudoperonospora cubensis: A Model System for Management of Downy Mildews

Ojiambo

Gent

Quesada‐Ocampo

et al. 2015

Annu. Rev. Phytopathol.

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The resurgence of cucurbit downy mildew has dramatically influenced production of cucurbits and disease management systems at multiple scales. Long-distance dispersal is a fundamental aspect of epidemic development that influences the timing and extent of outbreaks of cucurbit downy mildew. The dispersal potential of Pseudoperonospora cubensis appears to be limited primarily by sporangia production in source fields and availability of susceptible hosts and less by sporangia survival during transport. Uncertainty remains regarding the role of locally produced inoculum in disease outbreaks, but evidence suggests multiple sources of primary inoculum could be important. Understanding pathogen diversity and population differentiation is a critical aspect of disease management and an active research area. Underpinning advances in our understanding of pathogen biology and disease management has been the research capacity and coordination of stakeholders, scientists, and extension personnel. Concepts and approaches developed in this pathosystem can guide future efforts when responding to incursions of new or reemerging downy mildew pathogens.

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Characterization of Pseudoperonospora cubensis isolates from Europe and Asia using ISSR and SRAP molecular markers

Cited by 25 publications

References 47 publications

Coincidence of virulence shifts and population genetic changes of Pseudoperonospora cubensis in the Czech Republic

Coincidence of virulence shifts and population genetic changes of Pseudoperonospora cubensis in the Czech Republic

Pseudoperonospora cubensis virulence and pathotype structure in Kazakhstan

Epidemiology and Population Biology of Pseudoperonospora cubensis: A Model System for Management of Downy Mildews

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