Characterization of Purine Hydroxylase I from Aspergillus nidulans

Mehra, Rekha; Coughlan, Michael P.

doi:10.1099/00221287-135-2-273

Cited by 5 publications

(6 citation statements)

References 24 publications

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“…For example, several genes for the basic generation of energy via glycolysis, the tricarboxylic acid cycle, and the pentose phosphate cycle were found, such as the genes coding for transketolases and transaldolases from the pentose phosphate cycle; glucose-6-phosphate dehydrogenases, fructose-1,6-bisphosphate dehydrogenase, and pyruvate carboxylases from the glycolysis process; and malate dehydrogenase and an aconitate hydratase from the tricarboxylic acid cycle. Five genes (2%) were found that are probably involved in the utilization of alternative nitrogen sources, including genes encoding the proline oxidase catalyzing the degradation of proline to glutamate (83), a choline oxidase, two xanthine oxidases responsible for the degradation of xanthine to urea (38,65), and an ␥-aminobutyrate transaminase catalyzing the formation of succinate semialdehyde and glutamate (57). , genes without significant expression changes.…”

Section: Resultsmentioning

confidence: 99%

“…Potential AreA-dependent transporters include the three ammonium permeases mentioned above and several amino acid and peptide transporters as well as permeases of other nitrogen compounds ( Table 2, category IIc). Four genes whose products may be involved in nitrogen and carbon metabolism include the glutamine synthetase (GS)-encoding gene glnA (75); a gene coding for the xanthine dehydrogenase, which is responsible for the utilization of xanthine as an alternative nitrogen source (38,65); and a glycerol dehydrogenase and a phosphatidyl transferase, probably involved in carbohydrate metabolism. Finally, this group also contained genes likely involved in secondary metabolite production.…”

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confidence: 99%

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Cross-Species Hybridization with Fusarium verticillioides Microarrays Reveals New Insights into Fusarium fujikuroi Nitrogen Regulation and the Role of AreA and NMR

et al. 2008

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In filamentous fungi, the GATA-type transcription factor AreA plays a major role in the transcriptional activation of genes needed to utilize poor nitrogen sources. In Fusarium fujikuroi, AreA also controls genes involved in the biosynthesis of gibberellins, a family of diterpenoid plant hormones. To identify more genes responding to nitrogen limitation or sufficiency in an AreA-dependent or -independent manner, we examined changes in gene expression of F. fujikuroi wild-type and ⌬areA strains by use of a Fusarium verticillioides microarray representing ϳ9,300 genes. Analysis of the array data revealed sets of genes significantly down-and upregulated in the areA mutant under both N starvation and N-sufficient conditions. Among the downregulated genes are those involved in nitrogen metabolism, e.g., those encoding glutamine synthetase and nitrogen permeases, but also those involved in secondary metabolism. Besides AreA-dependent genes, we found an even larger set of genes responding to N starvation and N-sufficient conditions in an AreA-independent manner. To study the impact of NMR on AreA activity, we examined the expression of several AreA target genes in the wild type and in areA and nmr deletion and overexpression mutants. We show that NMR interacts with AreA as expected but affects gene expression only in early growth stages. This is the first report on genome-wide expression studies examining the influence of AreA on nitrogen-responsive gene expression in a genome-wide manner in filamentous fungi.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Cross-Species Hybridization with Fusarium verticillioides Microarrays Reveals New Insights into Fusarium fujikuroi Nitrogen Regulation and the Role of AreA and NMR

et al. 2008

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“…Two enzymes of the XDH family have been described in A. nidulans. Purine hydroxylase I (PHI, HxA encoded by the hxA gene) is a typical XDH [7][8][9]. Purine hydroxylase II (PHII, HxnS; see below) has unprecedented substrate & 2017 The Authors.…”

Section: Introductionmentioning

confidence: 99%

A eukaryotic nicotinate-inducible gene cluster: convergent evolution in fungi and bacteria

et al. 2017

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Nicotinate degradation has hitherto been elucidated only in bacteria. In the ascomycete Aspergillus nidulans, six loci, hxnS/AN9178 encoding the molybdenum cofactor-containing nicotinate hydroxylase, AN11197 encoding a Cys2/His2 zinc finger regulator HxnR, together with AN11196/hxnZ, AN11188/hxnY, AN11189/hxnP and AN9177/hxnT, are clustered and stringently co-induced by a nicotinate derivative and subject to nitrogen metabolite repression mediated by the GATA factor AreA. These genes are strictly co-regulated by HxnR. Within the hxnR gene, constitutive mutations map in two discrete regions. Aspergillus nidulans is capable of using nicotinate and its oxidation products 6-hydroxynicotinic acid and 2,5-dihydroxypyridine as sole nitrogen sources in an HxnR-dependent way. HxnS is highly similar to HxA, the canonical xanthine dehydrogenase (XDH), and has originated by gene duplication, preceding the origin of the Pezizomycotina. This cluster is conserved with some variations throughout the Aspergillaceae. Our results imply that a fungal pathway has arisen independently from bacterial ones. Significantly, the neo-functionalization of XDH into nicotinate hydroxylase has occurred independently from analogous events in bacteria. This work describes for the first time a gene cluster involved in nicotinate catabolism in a eukaryote and has relevance for the formation and evolution of co-regulated primary metabolic gene clusters and the microbial degradation of N-heterocyclic compounds.

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“…Instead, we mined six genes for xanthine dioxygenase (XDO) based on the gene annotations. XDO can catalyze both hydroxylation of hypoxanthine to xanthine and hydroxylation of xanthine to uric acid 24,25 . XDO is a fungal enzyme that lacks a molybdopterin cofactor but is dependent on α-ketoglutarate and has the TauD motif 25 .…”

Section: Resultsmentioning

confidence: 99%

Genome sequence analysis of the fairy ring-forming fungus Lepista sordida and gene candidates for interaction with plants

Takano

Yamamoto

Suzuki

et al. 2019

Sci Rep

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Circular patterns called “fairy rings” in fields are a natural phenomenon that arises through the interaction between basidiomycete fungi and plants. Acceleration or inhibition of plant vegetative growth and the formation of mushroom fruiting bodies are both commonly observed when fairy rings form. The gene of an enzyme involved in the biosynthesis of these regulators was recently isolated in the fairy ring-forming fungus, Lepista sordida . To identify other genes involved in L. sordida fairy ring formation, we used previously generated sequence data to produce a more complete draft genome sequence for this species. Finally, we predicted the metabolic pathways of the plant growth regulators and 29 candidate enzyme-coding genes involved in fairy-ring formation based on gene annotations. Comparisons of protein coding genes among basidiomycete fungi revealed two nitric oxide synthase gene candidates that were uniquely encoded in genomes of fairy ring-forming fungi. These results provide a basis for the discovery of genes involved in fairy ring formation and for understanding the mechanisms involved in the interaction between fungi and plants. We also constructed a new web database F-RINGS ( http://bioinf.mind.meiji.ac.jp/f-rings/ ) to provide the comprehensive genomic information for L. sordida .

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Characterization of Purine Hydroxylase I from Aspergillus nidulans

Cited by 5 publications

References 24 publications

Cross-Species Hybridization with Fusarium verticillioides Microarrays Reveals New Insights into Fusarium fujikuroi Nitrogen Regulation and the Role of AreA and NMR

Cross-Species Hybridization with Fusarium verticillioides Microarrays Reveals New Insights into Fusarium fujikuroi Nitrogen Regulation and the Role of AreA and NMR

A eukaryotic nicotinate-inducible gene cluster: convergent evolution in fungi and bacteria

Genome sequence analysis of the fairy ring-forming fungus Lepista sordida and gene candidates for interaction with plants

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