Characterization of pyruvate kinase isolated from the adductor muscle of the mollusc Scapharca inaequivalvis

Cortesi, P.; Fabbri, Daniele; Isani, Gloria; Cattani, Otello; Carpenè, Emilio

doi:10.1016/0305-0491(85)90172-5

Cited by 5 publications

(1 citation statement)

References 14 publications

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“…In addition, molluscan pyruvate kinase is regulated by the phosphorylation/dephosphorylation process (Marie et al, 1979;Siebenaller 1979;Kiener and Westhead, 1980;Nakashima et al, 1982;Hakim et al, 1984;Holwerda et al, 1989;Carillo et al, 2001). Moreover, there are many physiological factors such as temperature, anaerobic conditions, tidal cycle, seasonal and ion concentration variations that can alter pyruvate kinase activity in molluscs (Cortesi and Carpene, 1981;Carpene et al, 1984;Hakim et al, 1984;Cortesi et al, 1985;Ibarguren et al, 1990;Simpfendorfer et al, 1997).…”

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confidence: 99%

Cadmium induces both pyruvate kinase and Na+/H+exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cells ofMytilus galloprovincialis(L.)

Dailianis

Kaloyianni

2004

Journal of Experimental Biology

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SUMMARYThe present study investigates the transduction pathway mediated by cadmium in isolated digestive gland cells of mussel Mytilus galloprovincialis. The effects of cadmium treatment on a key glycolytic enzyme, pyruvate kinase (PK), and on Na+/H+ exchanger activity were examined. Cadmium (50 μmol l–1) caused a significant elevation of intracellular pH (pHi) and a rise (176%) of Na influx relative to control values. The amiloride analogue, EIPA (20 nmol l–1), a Na+/H+ exchanger blocker,together with cadmium, significantly reduced the effect of treatment by cadmium alone on both Na+ influx and pHi. In addition, PK activity was significantly increased after treatment with cadmium. PK activity was inhibited after treatment of cells with amiloride or EIPA together with cadmium. Moreover, phorbol-ester (PMA), a potent activator of protein kinase C(PKC), caused a significant rise in both pHi and PK activity, while staurosporine or calphostin C reversed both events. Adrenaline, isoprenaline and phenylephrine alone or together with cadmium also significantly increased the pHi and PK activity of isolated digestive gland cells. The latter effectors in combination with cadmium showed a synergistic effect on pHi and PK. These responses seem to be blocked by propranolol, metoprolol and prazosin. Our findings suggest a hormone-like effect of cadmium on digestive gland cells. The signal transduction pathway induced by cadmium involves the stimulation of PK, PKC and Na+/H+ exchanger in isolated digestive gland cells of Mytilus galloprovincialis.

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