Characterization of quinolone resistance in Salmonella spp. isolates from food products and human samples in Brazil

Pribul, Bruno Rocha; Festivo, Márcia Lima; Souza, Miliane Moreira Soares de; Rodrigues, Dália dos Prazeres

doi:10.1016/j.bjm.2015.04.001

Cited by 34 publications

(30 citation statements)

References 26 publications

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“…Nevertheless, plasmid‐mediated quinolone resistance in Salmonella is an alternative resistance mechanism that employs an enzyme to shield DNA gyrase and type IV topoisomerase from quinolone inhibition (Al‐Gallas et al, ; van Hoek et al, ). The horizontal transmission of qnr genes increases resistance to quinolones such as ciprofloxacin, which could explain why one serovar was only resistant to ciprofloxacin but not to nalidixic acid (Aldred et al, ; Ata, Yibar, Arslan, Mustak, & Gunaydin, ; Cavaco & Aarestrup, ; Ferrari et al, ; Pribul et al, ).…”

Section: Discussionmentioning

confidence: 99%

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Salmonellosis detection and evidence of antibiotic resistance in an urban raccoon population in a highly populated area, Costa Rica

Baldi

Barquero-Calvo

Hutter

et al. 2019

Zoonoses and Public Health

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Wild animals are involved in zoonotic disease transmission cycles. These are generally complex and poorly understood, especially among animals adapted to life in human ecosystems. Raccoons are reservoirs and effective carriers for infectious agents such as Salmonella throughout different environments and contribute to the transference of resistance genes. This study examined the presence of circulating Salmonella sp. in a population of raccoons in a tropical urban environment and evaluated resistance to antibiotics commonly used to treat salmonellosis. A total of 97 raccoons of different ages and sex were included in this study. 49% (38–60 CI) of the faecal samples were positive for Salmonella spp. The study identified 15 circulating serovars with the most prevalent being S. Hartford (7/15), S. Typhimurium (4/15) and S. Bovismorbificans (4/15). These serovars correspond to the serovars detected in humans with clinical symptoms in Costa Rica. 9.5% of the Salmonella strains recovered demonstrated ciprofloxacin resistance, and 7.1% showed resistance to nalidixic acid. This study provides evidence of multiple Salmonella serovars circulating in a population of urban raccoons in Costa Rica. Furthermore, the study confirms the existence of antimicrobial resistance to two antibiotics used to treat human salmonellosis. The findings emphasize the role of the raccoon as a reservoir of Salmonella in the Greater Metropolitan Area of Costa Rica (GAM) and stress the need for active monitoring of the presence and possible spread in antibiotic resistance due to this peri‐domestic carnivore.

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Section: Discussionmentioning

confidence: 99%

“…The horizontal transmission of qnr genes increases resistance to quinolones such as ciprofloxacin, which could explain why one serovar was only resistant to ciprofloxacin but not to nalidixic acid (Aldred et al, 2014;Ata, Yibar, Arslan, Mustak, & Gunaydin, 2014;Cavaco & Aarestrup, 2009;Ferrari et al, 2013;Pribul et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Salmonellosis detection and evidence of antibiotic resistance in an urban raccoon population in a highly populated area, Costa Rica

Baldi

Barquero-Calvo

Hutter

et al. 2019

Zoonoses and Public Health

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“…; Pribul et al . ); for intron (Firoozeh et al . ) and for efflux pumps ( qnrA, qnrB, qnrS, qnrC, QnrD, AAC(6′)‐Ib‐cr, armA, rmtA, rmtB, rmtC, rmtD, npmA, qepA, qacE‐sul1 ) (Whichard et al .…”

Section: Methodsmentioning

confidence: 99%

“…Different genes reported for conferring antimicrobial resistance to various drugs were targeted: for beta-lactams (carbapenems, cephalosporins, penicillins) (bla TEM-1 , (Firoozeh et al 2012) and for efflux pumps (qnrA, qnrB, qnrS, qnrC, QnrD, AAC(6 0 )-Ib-cr, armA, rmtA, rmtB, rmtC, rmtD, npmA, qepA, qacE-sul1) (Whichard et al 2007;Ma et al 2009;Al Sheikh et al 2014;Pribul et al 2016). The primer sequences and annealing temperatures are described in Table 1.…”

Section: Detection Of Drug Resistance Genesmentioning

confidence: 99%

Detection and characterization of multidrug‐resistant Salmonella enterica serovar Infantis as an emerging threat in poultry farms of Faisalabad, Pakistan

et al. 2019

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Aims The aim of this study was the molecular identification of Salmonella enterica serovar Infantis (S. Infantis) isolated from poultry samples and their antimicrobial resistance and virulence profiling. Methods and Results A total of 149 isolates, belonging to genus Salmonella, originally isolated from 340 suspected poultry post mortem specimens reported by us earlier were preliminary identified as Salmonella by biochemical methods and confirmed by polymerase chain reaction targeting genus‐specific gene invA. Targeting serovar‐specific gene fragment (fljB) resulted in confirmation of 54 isolates as S. Infantis which were further confirmed by sequencing of 16S RNA and fljB genes. Swimming and swarming motilities were detected in 98·1 and 11·1% isolates respectively. Phenotypic disc diffusion assay against 23 antimicrobial agents showed the highest resistance against pefloxacin (PEF) (94·4%), chloramphenicol (83·3%) and imipenem (77·7%) while 5·3% isolates showed extended‐spectrum β‐lactamase production. Fifty‐nine genes reported for antimicrobial resistance and 12 for conferring virulence were targeted. The most prevalent resistance gene for aminoglycosides was aadA (42·3%), for quinolone resistance determining region parE (62·5%), for penicillin's Int1 (62·9%), for chloramphenicol cat3 (66·1%) and for beta‐lactams blaTEM‐1 (44·4%). Among efflux pump coding genes, armA showed highest (74·2%) prevalence and for virulence, a high prevalence of SopE (89·2%) showed the zoonotic potential of the isolates. The activity of efflux pumps was detected through Ethidium Bromide‐agar method. Conclusions Poultry could act as reservoirs of multidrug resistance Salmonella. Significance and Impact of the Study We firstly report the prevalence and molecular characterization of virulence/drug resistance in S. Infantis from this region and the results may contribute to designing precisely targeted therapy. This study has also highlighted the possible emergence of S. Infantis with zoonotic potential.

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“…According to Pribul et al (2016), this test was performed using representatives of the quinolone class (OXOID) for human and veterinary therapeutic use, such as Nalidixic Acid (NAL), Ciprofloxacin (CIP), Enrofloxacin (ENO), Ofloxacin (OFL), and Levofloxacin (LVX).…”

Section: Methodsmentioning

confidence: 99%

Characteristics of Quinolone Resistance in Salmonella spp. Isolates from the Food Chain in Brazil

et al. 2017

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Salmonella spp. is an important zoonotic pathogen related to foodborne diseases. Despite that quinolones/fluoroquinolones are considered a relevant therapeutic strategy against resistant isolates, the increase in antimicrobial resistance is an additional difficulty in controlling bacterial infections caused by Salmonella spp. Thus, the acquisition of resistance to quinolones in Salmonella spp. is worrisome to the scientific community along with the possibility of transmission of resistance through plasmids. This study investigated the prevalence of plasmid-mediated quinolone resistance (PMQR) in Salmonella spp. and its association with fluoroquinolone susceptibility in Brazil. We evaluated 129 isolates, 39 originated from food of animal sources, and 14 from environmental samples and including 9 from animals and 67 from humans, which were referred to the National Reference Laboratory of Enteric Diseases (NRLEB/IOC/RJ) between 2009 and 2013. These samples showed a profile of resistance for the tested quinolones/fluoroquinolones. A total of 33 serotypes were identified; S. Typhimurium (63) was the most prevalent followed by S. Enteritidis (25). The disk diffusion test showed 48.8% resistance to enrofloxacin, 42.6% to ciprofloxacin, 39.53% to ofloxacin, and 30.2% to levofloxacin. According to the broth microdilution test, the resistance percentages were: 96.1% to nalidixic acid, 64.3% to enrofloxacin, 56.6% to ciprofloxacin, 34.1% to ofloxacin, and 30.2% to levofloxacin. Qnr genes were found in 15 isolates (8 qnrS, 6 qnrB, and 1 qnrD), and the aac(6′)-Ib gene in 23. The integron gene was detected in 67 isolates with the variable region between ±600 and 1000 bp. The increased detection of PMQR in Salmonella spp. is a serious problem in Public Health and must constantly be monitored. Pulsed-field gel electrophoresis was performed to evaluated clonal profile among the most prevalent serovars resistant to different classes of quinolones. A total of 33 pulsotypes of S. Typhimurium were identified with a low percentage of genetic similarity (≤65%). This result demonstrates the presence of high diversity in the resistant clones evaluated in this study.

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Characterization of quinolone resistance in Salmonella spp. isolates from food products and human samples in Brazil

Cited by 34 publications

References 26 publications

Salmonellosis detection and evidence of antibiotic resistance in an urban raccoon population in a highly populated area, Costa Rica

Salmonellosis detection and evidence of antibiotic resistance in an urban raccoon population in a highly populated area, Costa Rica

Detection and characterization of multidrug‐resistant Salmonella enterica serovar Infantis as an emerging threat in poultry farms of Faisalabad, Pakistan

Characteristics of Quinolone Resistance in Salmonella spp. Isolates from the Food Chain in Brazil

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