Characterization of rabbit urine-derived stem cells for potential application in lower urinary tract tissue regeneration

Yang, Huai; Chen, Bote; Deng, Jing; Zhuang, Guiwu; Wu, Shijian; Liu, Guihua; Deng, Chao; Yang, Guosheng; Qiu, Xiaoyu; Wei, Pinqing; Wang, Xisheng; Zhang, Yuanyuan

doi:10.1007/s00441-018-2885-z

Cited by 21 publications

(19 citation statements)

References 27 publications

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“…Urine-derived stem cells (USCs) are emerging as a promising cell resource for their multiple applications in cell therapy and tissue engineering due to their non-invasive obtaining procedure, potent proliferation capabilities, and ability to serve as original cells for reprogramming into disease-specific iPSCs (Ji et al, 2017;Wu et al, 2019). In addition, since USCs are highly homologous with the urinary system, they have a promising potential in bladder tissue regeneration and urethral reconstruction and can effectively reduce inflammation and fibrosis (Yang et al, 2018). Shou Fu et al suggested that USCs may promote kidney repair and improve function following ischemic AKI (Tian et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Transfer of MicroRNA-216a-5p From Exosomes Secreted by Human Urine-Derived Stem Cells Reduces Renal Ischemia/Reperfusion Injury

Zhang

Wang

Yang

et al. 2020

Front. Cell Dev. Biol.

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Human urine-derived stem cells (USCs) protect rats against kidney ischemia/reperfusion (I/R) injury. Here we investigated the role of USCs exosomes (USCs-Exos) in protecting tubular endothelial cells and miRNA transfer in the kidney. Human USCs and USCs-Exos were isolated and verified by morphology and specific biomarkers. USC-Exos played a protective role in human proximal tubular epithelial cells (HK-2) exposed to hypoxia/reoxygenation (H/R). USCs-Exos were rich in miR-216a-5p, which targeted phosphatase and tensin homolog (PTEN) and regulated cell apoptosis through the Akt pathway. In HK-2 cells exposed to H/R, incubation with USC-Exos increased miR-216-5p, decreased PTEN levels, and stimulated Akt phosphorylation. Exposure of hypoxic HK-2 cells to USCs-Exos pretreated with anti-miR-216a-5p can prevent the increase of miR-216-5p and Akt phosphorylation levels, restore PTEN expression, and promote apoptosis. The dual-luciferase reported gene assay in HK-2 cells confirmed that miR-216a-5p targeted PTEN. In rats with I/R injury, intravenous infusion of USCs-Exos can effectively induce apoptosis suppression and functional protection, which is associated with decreased PTEN. Infusion of exosomes from anti-miR-216a-5p-transfected USCs weakened the protective effect in the I/R model. Therefore, USCs-Exos can reduce renal I/R injury by transferring miR-216a-5p targeting PTEN. Potentially, USCs-Exos rich in miR-216a-5p can serve as a promising therapeutic option for AKI.

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Section: Introductionmentioning

confidence: 99%

Transfer of MicroRNA-216a-5p From Exosomes Secreted by Human Urine-Derived Stem Cells Reduces Renal Ischemia/Reperfusion Injury

Zhang

Wang

Yang

et al. 2020

Front. Cell Dev. Biol.

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“…The median age was 11 years (range 10-14) at the time of surgery and median follow-up was 71 months (range 36-76 months. The median end maximum urinary flow rate was 27.1 mL/s (range [16][17][18][19][20][21][22][23][24][25][26][27][28], and serial radiographic and endoscopic studies showed the maintenance of wide urethral calibres without strictures. Urethral biopsies showed that the engineered grafts had developed a normal appearing architecture by 3 months after implantation [29].…”

Section: Tissue Engineeringmentioning

confidence: 97%

“…Another approach was the use of acellular bladder matrix (ABM) harvested from porcine or leporine bladder and seeded with autologous oral keratinocytes, adipose-derived stem cells, autologous bladder smooth muscle cells and urothelial cells [ 19 , 20 ] and likewise, acellular bladder submucosa matrix (BSM) seeded with autologous minced urethral tissue linked to BSM with fibrin glue [ 21 ]. More recently, the use of urine-derived stem cells in a rabbit model has been reported with the potential for this being of value for future research in this area [ 22 ]. Translation of these techniques from basic experiments to clinical experience poses numerous challenges for the future.…”

Section: Tissue Engineeringmentioning

confidence: 99%

Tissue engineering of the urethra: where are we in 2019?

Chapple

2019

World J Urol

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Purpose The purpose of this review is to assess the potential role of tissue engineering for urethral reconstruction. It is well-recognised that urethrotomy remains the first-line therapy in the treatment of urethral stricture. Following on from the randomised study which recommended no difference between urethrotomy and urethral dilation, Steenkamp et al. reported long-term success rates of only 20%. Patients with longer strictures, penile or distal urethral strictures, and extensive periurethral spongiofibrosis typically do not respond well to repeated incisions. This report reviews the potential role of tissue engineering as applied to augmentation urethroplasty, which is the treatment of choice following failed urethrotomy. Methods A review of the literature was carried out. The principal emphasis was on tissue engineering as applied to augmentation urethroplasty, but an introductory section reviews the use of urethrotomy and the background to contemporary practise with augmentation urethroplasty using oral mucosa. Results It is evident that a cellular matrix which requires the ingrowth of cells is unlikely to be successful except for very short strictures. Other approaches such as injection of stem cells have not been adequately trialled in humans to date. Tissueengineered substitute for autologous oral mucosa has been used and the results relating to this are reviewed. Conclusions Tissue engineering of autologous tissue for urethroplasty is expensive. It is unnecessary for the majority of cases, but could be potentially useful for very lengthy strictures, for instance, relating to lichen sclerosis. Whilst tissueengineered oral mucosa has been successfully used, a great deal more work would be necessary to develop an appropriate matrix. Another study has looked at a larger series using an alternative tissue-engineered substitute, but the results have been very disappointing. At present, it has to be concluded that there is no effective and validated tissue engineering solution for the management of urethral stricture disease.

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“…Mesenchymal stem cells were also positive for CD90 and CD45. Yang et al [67] used flow cytometry, immunocytochemistry, and cell proliferation assay to characterize UDSCs harvested from rabbits through either voided urine or bladder washes. Successful differentiation into urothelial, smooth muscle, and osteogenic cell lines was achieved.…”

Section: Urine-derived Stem Cells In Urethral Regenerationmentioning

confidence: 99%

Urine as a Main Effector in Urological Tissue Engineering—A Double-Edged Sword

Abbas

Ali

Uddin

2020

Cells

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In order to reconstruct injured urinary tract tissues, biodegradable scaffolds with autologous seeded cells are explored in this work. However, when cells are obtained via biopsy from individuals who have damaged organs due to infection, congenital disorders, or cancer, this can result in unhealthy engineered cells and donor site morbidity. Thus, neo-organ construction through an alternative cell source might be useful. Significant advancements in the isolation and utilization of urine-derived stem cells have provided opportunities for this less invasive, limitless, and versatile source of cells to be employed in urologic tissue-engineered replacement. These cells have a high potential to differentiate into urothelial and smooth muscle cells. However, urinary tract reconstruction via tissue engineering is peculiar as it takes place in a milieu of urine that imposes certain risks on the implanted cells and scaffolds as a result of the highly cytotoxic nature of urine and its detrimental effect on both growth and differentiation of these cells. Both of these projections should be tackled thoughtfully when designing a suitable approach for repairing urinary tract defects and applying the needful precautions is vital.

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Characterization of rabbit urine-derived stem cells for potential application in lower urinary tract tissue regeneration

Cited by 21 publications

References 27 publications

Transfer of MicroRNA-216a-5p From Exosomes Secreted by Human Urine-Derived Stem Cells Reduces Renal Ischemia/Reperfusion Injury

Transfer of MicroRNA-216a-5p From Exosomes Secreted by Human Urine-Derived Stem Cells Reduces Renal Ischemia/Reperfusion Injury

Tissue engineering of the urethra: where are we in 2019?

Urine as a Main Effector in Urological Tissue Engineering—A Double-Edged Sword

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