Characterization of radiation sensitivity of human squamous carcinoma A431 cells

Ng, C. E.; Keng, Peter C.; Sutherland, Robert M.

doi:10.1038/bjc.1987.193

Cited by 17 publications

(4 citation statements)

References 18 publications

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“…Repopulation of tumor cells during treatment, tumor hypoxia, and resistance to radio-C therapy have all been implicated as causes of treatment failure after primary radiotherapy. [1][2][3] Accelerated fractionation irradiation, which shortens the total time of treatment, has been employed to increase the probability of locoregional control by reducing the risk of tumor repopulation. With hyperfractionated irradiation, multiple small fractions of radiation are given each day to increase the total dose but not the risk of long-term toxicity.…”

Section: Discussionmentioning

confidence: 99%

Hyperfractionated Irradiation with or without Concurrent Chemotherapy for Locally Advanced Head and Neck Cancer

et al. 1998

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Section: Discussionmentioning

confidence: 99%

Hyperfractionated Irradiation with or without Concurrent Chemotherapy for Locally Advanced Head and Neck Cancer

et al. 1998

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“…It is reported that retinoic acid compounds play an important role in cell proliferation, diferentiation, and skin infammation and have a strong role in inducing diferentiation and inhibiting proliferation of tumor cells [38]. Gong [39] showed that combined application of retinoic acid and TNF-α can activate the expression of Caspase-3 protein, increase the expression of Caspase-3, and promote the apoptosis of human epidermal squamous cell carcinoma cell A431, which is a feasible way to treat squamous cell carcinoma. Pubescenol is a lactone.…”

Section: Discussionmentioning

confidence: 99%

UPLC-Q-Exactive-MS Combined with Network Pharmacology to Explore the Antitumor Effect of Polygonatum sibiricum Leaf Tea

Chen,

Xia,

Yin

et al. 2023

Journal of Food Biochemistry

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Background. Currently, the substance basis and function of Polygonatum sibiricum leaf remain unknown. Objective. This study aims to investigate the antitumor mechanism of P. sibiricum leaf tea through network pharmacology. Methods. Ultraperformance liquid chromatography quadrupole exactive mass spectrometry (UPLC-Q-Exactive-MS) was employed to analyze the chemical components in the extract of P. sibiricum leaf tea. Compounds were screened using the PubChem database and SwissADME platform. The compound targets were identified using the Swiss Target Prediction database, while the disease targets were obtained from the GeneCards database. Subsequently, a compound-protein interaction network was constructed, and KEGG pathway enrichment analysis was performed to elucidate the antitumor activity pathway of P. sibiricum leaf tea. Molecular docking verification was carried out as well. Results. From the extract of P. sibiricum leaf tea, a total of fifty-six components were screened, including 27 active components. Seventy-two corresponding targets closely associated with the antitumor activity of P. sibiricum leaf tea were identified. By constructing a compound-target-pathway network diagram, three key compounds (pubescenol, avenanthramide E, and 13-cis-acitretin) and eleven key targets (AKT1, EGFR, CASP3, CCND1, MTOR, MMP9, ERBB2, BCL2L1, MAPK1, PPARG, and PIK3CA) were determined. Additionally, KEGG pathway analysis identified 30 antitumor related pathways. The results of molecular docking between the three key compounds and the top three targets (AKT1, EGFR, and CASP3) were consistent with the findings of network pharmacology. Conclusion. This study highlights the distinctive features of P. sibiricum leaf tea extract, which possesses multiple components, targets, and pathways. It successfully identifies the active components of P. sibiricum leaf tea that exhibit antitumor properties, along with their potential mechanisms of action. These findings offer valuable insights and inspiration for further research on novel mechanisms of action.

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“…Both in vivo and ex vivo analysis revealed that the tumor line responded to the single high dose fraction of radiation. A 10 Gy dose was chosen based on an earlier study characterizing the radiation sensitivity of human squamous carcinoma A431 cells 26 . The use of in vivo CELI to track the diffusive spread of phosphorescent tattoo ink as a measure of treatment response in an A431 mouse xenograft model was successful after two days.…”

Section: Discussionmentioning

confidence: 99%

Tracking tumor radiotherapy response in vivo with Cherenkov-excited luminescence ink imaging

et al. 2020

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Purpose: This study demonstrates remote imaging for in vivo detection of radiation-induced tumor microstructural changes by tracking the diffusive spread of injected intratumor UV excited tattoo ink using Cherenkov-excited luminescence imaging (CELI). Methods: Micro-liter quantities of luminescent tattoo ink with UV absorption and visible emission were injected at a depth of 2mm into mouse tumors prior to receiving a high dose treatment of radiation. X-rays from a clinical linear accelerator were used to excite phosphorescent compounds within the tattoo ink through Cherenkov emission. The in vivo phosphorescence was detected using a time-gated intensified CMOS camera immediately after injection, and then again at varying time points after the ink had broken down with the apoptotic tumor cells. ex vivo tumors were imaged post-mortem using hyperspectral cryo-fluorescence imaging to quantify necrosis and compared to Cherenkov-excited light imaging of diffusive ink spread measured in vivo. Results: Imaging of untreated control mice showed that ink distributions remained constant after four days with less than 3% diffusive spread measured using full width at 20% max. For all mice, in vivo CELI measurements matched within 12% of the values estimated by the high-resolution ex vivo sliced luminescence imaging of the tumors. The tattoo ink spread in treated mice was found to correlate well with the nonperfusion necrotic core volume (R 2 =0.92) but not well with total tumor volume changes (R 2 =0.34). Conclusion: In vivo and ex vivo findings indicate that the diffusive spread of the injected tattoo ink can be related to radiation-induced necrosis, independent of total tumor volume change. Tracking the diffusive spread of the ink allows for distinguishing between an increase in tumor size due to new cellular growth and an increase in tumor size due to edema. Furthermore, the imaging resolution of CELI allows for in vivo tracking of subtle microenvironmental changes which occur earlier than tumor shrinkage and this offers the potential for novel, minimally invasive radiotherapy response assay without interrupting a singular clinical workflow.

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Characterization of radiation sensitivity of human squamous carcinoma A431 cells

Cited by 17 publications

References 18 publications

Hyperfractionated Irradiation with or without Concurrent Chemotherapy for Locally Advanced Head and Neck Cancer

Hyperfractionated Irradiation with or without Concurrent Chemotherapy for Locally Advanced Head and Neck Cancer

UPLC-Q-Exactive-MS Combined with Network Pharmacology to Explore the Antitumor Effect of Polygonatum sibiricum Leaf Tea

Tracking tumor radiotherapy response in vivo with Cherenkov-excited luminescence ink imaging

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