Characterization of radioligand binding to a transmembrane receptor reconstituted into Lipobeads

Park, Paul S.‐H.; Ng, Charlene Cheuk Wing; Buck, Sean; Wells, James W.; Cheng, Yu; Pennefather, Peter

doi:10.1016/j.febslet.2004.03.124

Cited by 9 publications

(10 citation statements)

References 23 publications

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“…Liposomes were first reported for drug delivery in 1965 72. Liposome‐based templates include lipospheres and lipobeads 73–78. Liposome probes comprise an outer phospholipid layer forming a spherical vesicle that has an aqueous core, while lipobeads possess an inner polymeric core (see Figure 3 for examples of liposomes, lipobeads, and other non‐protein‐based DDS that have previously been developed).…”

Section: Liposome‐based Nanomedicine Platformmentioning

confidence: 99%

Protein‐Based Nanomedicine Platforms for Drug Delivery

Maham

Tang

Wang

et al. 2009

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Protein-based nanomedicine platforms for drug delivery comprise naturally self-assembled protein subunits of the same protein or a combination of proteins making up a complete system. They are ideal for drug-delivery platforms due to their biocompatibility and biodegradability coupled with low toxicity. A variety of proteins have been used and characterized for drug-delivery systems, including the ferritin/apoferritin protein cage, plant-derived viral capsids, the small Heat shock protein (sHsp) cage, albumin, soy and whey protein, collagen, and gelatin. There are many different types and shapes that have been prepared to deliver drug molecules using protein-based platforms, including various protein cages, microspheres, nanoparticles, hydrogels, films, minirods, and minipellets. The protein cage is the most newly developed biomaterial for drug delivery and therapeutic applications. The uniform size, multifunctionality, and biodegradability push it to the frontier of drug delivery. In this Review, the recent strategic development of drug delivery is discussed with emphasis on polymer-based, especially protein-based, nanomedicine platforms for drug delivery. The advantages and disadvantages are also discussed for each type of protein-based drug-delivery system.

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Section: Liposome‐based Nanomedicine Platformmentioning

confidence: 99%

Protein‐Based Nanomedicine Platforms for Drug Delivery

Maham

Tang

Wang

et al. 2009

Small

464

375

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“…Our Lipobead membrane lipid composition was 48% ePC, 48% PS, and 4% cholesterol. This composition was selected in anticipation of experiments with transmembrane receptor constituted proteoliposomes (see Park et al, 2004). Thus, the high fraction of phosphatidylserine used here may have further restricted probe diffusivity.…”

Section: Figurementioning

confidence: 99%

Properties of a Self-Assembled Phospholipid Membrane Supported on Lipobeads

Cheng

Pennefather

2004

Biophysical Journal

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The overall objective of our work was to make a hydrogel-supported phospholipid bilayer that models a cytoskeleton-supported cell membrane and provides a platform for studying membrane biology. Previously, we demonstrated that a pre-Lipobead, consisting of phospholipids covalently attached to the surface of a hydrogel, could give rise to a Lipobead when incubated with liposomes because the attached phospholipids promote self-assembly of a phospholipid membrane on the pre-Lipobead. We now report the properties of that Lipobead membrane. The lateral diffusion coefficient of fluorescently labeled phosphatidylcholine analogs in the membrane was measured by fluorescence recovery after photobleaching and was found to decrease as the surface anchor density and hydrogel crosslinking density increased. Results from the quenching of phosphatidylcholine analogs suggest that the phospholipid membrane of the Lipobead was composed mostly of a semipermeable lipid bilayer. However, the diffusional barrier properties of the Lipobead membrane were demonstrated by the entrapment of 1.5-3.0 K dextran molecules in the hydrogel core after liposome fusion. This hydrogel-supported bilayer membrane preparation shows promise as a new platform for studying membrane biology and for high throughput drug screening.

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“…With the latter two methods, the lipobeads of 1-μm diameter are produced [2,7,23,25,28,31]. To prepare giant lipobeads with a diameter up to 100 μm, the inverse suspension polymerization (ISP) method is commonly applied [6,24,35,[43][44][45].…”

Section: Hydrogel/liposome Mixingmentioning

confidence: 99%

“…The first proteo-lipobeads were prepared when transmembrane receptors were reconstituted into the lipid bilayer of lipobeads [45]. It was found that the receptors retained their nativespecific binding.…”

Section: Proteins Within Lipid Bilayermentioning

confidence: 99%

“…Herein, it was found that permeability of the lipid membrane was similar to the free bilayer. To incorporate transmembrane proteins into the peripheral membrane of lipobeads, the hydrogels core particles were mixed and incubated with liposomes containing the proteins of interest within their lipid bilayer [45].…”

Section: Loading Of Lipobeads Prepared By Hydrogel/liposome Mixingmentioning

confidence: 99%

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Lipobeads

Казаков¹

2017

Liposomes

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A combination of lipid bilayer and cross-linked polymer network is the logical step in development of polymeric and liposomal nanoscopic systems to provide the natural level of functionality. From liposomal systems, lipobeads borrow the welldeveloped methods for preparation, diversity of lipids to control the properties of a lipid bilayer, biocompatibility of the lipid bilayer, possibility to vary size and morphology (passive targeting), availability of the external surface for attachment of various ligands (active targeting), encapsulation efficiency of both hydrophilic and hydrophobic molecules. Mechanical stability of the lipid bilayer and environmental responsiveness of the whole structure are the properties that hydrogel core brings to the new construct. The reports reviewed in this chapter demonstrate that lipobeads of nanometer and micrometer sizes can be prepared in different media, retain their stimuli responsiveness under physiological conditions, exhibit both reversible and irreversible aggregation, can be loaded with both small and high molecular weight molecules. As a platform for drug delivery systems, lipobeads have already been loaded with chemotherapeutics, malaria vaccine, and dermatological agent providing different controlled release profiles without leakage. Consecutive multistep triggering, new schemes of drug release, combined drug delivery, vesobeads, proteolipobeads, enzyme-containing lipobeads, and hemi-lipobeads are the directions for their future development.

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Characterization of radioligand binding to a transmembrane receptor reconstituted into Lipobeads

Cited by 9 publications

References 23 publications

Protein‐Based Nanomedicine Platforms for Drug Delivery

Protein‐Based Nanomedicine Platforms for Drug Delivery

Properties of a Self-Assembled Phospholipid Membrane Supported on Lipobeads

Lipobeads

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