Characterization of rainbow trout (Oncorhynchus mykiss) intestinal microbiota and inflammatory marker gene expression in a recirculating aquaculture system

Mansfield, Graeme S.; Desai, Atul R.; Nilson, S. A.; Kessel, Andrew G. Van; Drew, M. D.; Hill, Janet E.

doi:10.1016/j.aquaculture.2010.07.014

Cited by 90 publications

(70 citation statements)

References 41 publications

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“…Previous evaluations of gut microbiota composition in wild rainbow trout identified many of the bacterial genera that we observed in the shared core in this study (e.g., Aeromonas, Acinetobacter, Escherichia, Pseudomonas, Streptococcus, and Enterococcus) (50). These and many other genera observed within the shared core reported here have also been identified in cultureindependent and culture-based evaluations of gut microbiota composition in aquacultured trout and other salmonids (9,22,30,(52)(53)(54)(55)(56)(57). However, these previous reports did not identify these genera in all animals within the respective studies.…”

Section: Discussionsupporting

confidence: 55%

“…Our current information on the gut microbiota of rainbow trout (Oncorhynchus mykiss) is derived from analysis of culturable microorganisms (22)(23)(24) and culture-independent studies using fingerprinting and sequencing of 16S rRNA and other microbial genes (9,23,(25)(26)(27). These studies have revealed that the rainbow trout gut microbiota is dominated by the bacterial phyla Proteobacteria and Firmicutes, the same phyla that dominate the intestines of many other fishes (16,28,29).…”

mentioning

confidence: 99%

“…In contrast to these methodologies, high-throughput pyrosequencing of 16S rRNA genes permits unbiased identification of rare, as well as abundant, bacterial members of the gut microbiota at low cost per sequence. The gut microbiota of aquacultured trout has previously been analyzed by pyrosequencing of the cpn60 gene (9,30), but not the more commonly studied 16S rRNA gene. In this study, we tested whether long-term differences in rearing density and diet, alone and in combination, lead to alterations in animal performance, welfare, fillet quality, or gut microbiota using 16S rRNA gene pyrosequencing.…”

mentioning

confidence: 99%

“…Much research has focused on all-plant-protein diets and their impact on fish performance (4), palatability (5), and digestibility (6); water quality (7); intestinal inflammation (8); and the community of microorganisms residing in the intestine (microbiota) (9). Overall, significant advances have been made in alternative protein diet formulations in recent years, so that the growth performance of fish fed grain-based diets has been reported to be comparable to that of fish fed traditional fishmeal-based diets (7,10).…”

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confidence: 99%

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Aquacultured Rainbow Trout (Oncorhynchus mykiss) Possess a Large Core Intestinal Microbiota That Is Resistant to Variation in Diet and Rearing Density

Wong

Waldrop

Summerfelt

et al. 2013

Appl Environ Microbiol

202

181

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h As global aquaculture fish production continues to expand, an improved understanding of how environmental factors interact in fish health and production is needed. Significant advances have been made toward economical alternatives to costly fishmealbased diets, such as grain-based formulations, and toward defining the effect of rearing density on fish health and production. Little research, however, has examined the effects of fishmeal-and grain-based diets in combination with alterations in rearing density. Moreover, it is unknown whether interactions between rearing density and diet impact the composition of the fish intestinal microbiota, which might in turn impact fish health and production. We fed aquacultured adult rainbow trout (Oncorhynchus mykiss) fishmeal-or grain-based diets, reared them under high-or low-density conditions for 10 months in a single aquaculture facility, and evaluated individual fish growth, production, fin indices, and intestinal microbiota composition using 16S rRNA gene sequencing. We found that the intestinal microbiotas were dominated by a shared core microbiota consisting of 52 bacterial lineages observed across all individuals, diets, and rearing densities. Variations in diet and rearing density resulted in only minor changes in intestinal microbiota composition despite significant effects of these variables on fish growth, performance, fillet quality, and welfare. Significant interactions between diet and rearing density were observed only in evaluations of fin indices and the relative abundance of the bacterial genus Staphylococcus. These results demonstrate that aquacultured rainbow trout can achieve remarkable consistency in intestinal microbiota composition and suggest the possibility of developing novel aquaculture strategies without overtly altering intestinal microbiota composition.

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Section: Discussionsupporting

confidence: 55%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Aquacultured Rainbow Trout (Oncorhynchus mykiss) Possess a Large Core Intestinal Microbiota That Is Resistant to Variation in Diet and Rearing Density

Wong

Waldrop

Summerfelt

et al. 2013

Appl Environ Microbiol

202

181

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“…Wong et al (Wong et al, 2013) found a core of intestinal bacteria that were resistant to dietary changes in rainbow trout. However, several other studies demonstrated the impact of feed on gut microbiota in rainbow trout, especially linked to the introduction of vegetable ingredients (Desai et al, 2012;Heikkinen et al, 2006;Mansfield et al, 2010;Merrifield et al, 2009;Navarrete et al, 2012). In our study, the high dissimilarity between individual bacterial profiles does not allow us to confirm the hypothetical core microbiota in rainbow trout, but DGGE can detect only the most abundant taxa.…”

Section: Long-term Effects Of the Early Nutritional Stimulicontrasting

confidence: 43%

High or low dietary carbohydrate:protein ratios during first-feeding affect glucose metabolism and intestinal microbiota in juvenile rainbow trout

Geurden

Mennigen

Plagnes-Juan

et al. 2014

Journal of Experimental Biology

104

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Based on the concept of nutritional programming in mammals, we tested whether an acute hyperglucidic-hypoproteic stimulus during first feeding could induce long-term changes in nutrient metabolism in rainbow trout. Trout alevins received during the five first days of exogenous feeding either a hyperglucidic (40% gelatinized starch + 20% glucose) and hypoproteic (20%) diet (VLP diet) or a high-protein (60%) glucose-free diet (HP diet, control). Following a common 105-day period on a commercial diet, both groups were then challenged (65 days) with a carbohydrate-rich diet (28%). Short-and long-term effects of the early stimuli were evaluated in terms of metabolic marker gene expressions and intestinal microbiota as initial gut colonisation is essential for regulating the development of the digestive system. In whole alevins (short term), diet VLP relative to HP rapidly increased gene expressions of glycolytic enzymes, while those involved in gluconeogenesis and amino acid catabolism decreased. However, none of these genes showed persistent molecular adaptation in the liver of challenged juveniles (long term). By contrast, muscle of challenged juveniles subjected previously to the VLP stimulus displayed downregulated expression of markers of glycolysis and glucose transport (not seen in the short term). These fish also had higher plasma glucose (9 h postprandial), suggesting impaired glucose homeostasis induced by the early stimulus. The early stimulus did not modify the expression of the analysed metabolism-related microRNAs, but had short-and long-term effects on intestinal fungi (not bacteria) profiles. In summary, our data show that a short hyperglucidic-hypoproteic stimulus during early life may have a long-term influence on muscle glucose metabolism and intestinal microbiota in trout. KEY WORDS: Nutritional programming, Rainbow trout, Carbohydrates, Protein, Metabolism INTRODUCTIONPrenatal or early nutritional neonatal events exerted during critical developmental windows may result in permanent changes in postnatal growth potential, health and metabolic status in mammals (Burdge and Lillycrop, 2010;Lucas, 1998;Patel and Srinivasan, 2002;Patel et al., 2009;Metges et al., 2014;Duque-Guimarães and Ozanne, 2013;Devaskar and Thamotharan, 2007). It has been RESEARCH ARTICLE 1 INRA, UR1067 Nutrition Metabolism and Aquaculture, F-64310 Saint-Pée-surNivelle, France. suggested that this process of developmental plasticity utilizes the early nutritional cues to prepare individual phenotypes to better match the predicted future nutritional environment (Gluckman et al., 2005). Possible biological mechanisms for 'imprinting' the nutritional event until adulthood in mammalian vertebrates comprise adaptive changes in gene expression pattern or cellular phenotype (epigenetic phenomenon), nutrient-sensitive signalling pathways and adaptive clonal selection, which may be transmitted to future offspring (Lucas, 1998;Symonds et al., 2009;Waterland and Jirtle, 2003;Gut and Verdin, 2013). Experimental data on the concept...

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The Gut Microbiota of Fish

Romero

Ringø

Merrifield

2014

Aquaculture Nutrition

146

105

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Characterization of rainbow trout (Oncorhynchus mykiss) intestinal microbiota and inflammatory marker gene expression in a recirculating aquaculture system

Cited by 90 publications

References 41 publications

Aquacultured Rainbow Trout (Oncorhynchus mykiss) Possess a Large Core Intestinal Microbiota That Is Resistant to Variation in Diet and Rearing Density

Aquacultured Rainbow Trout (Oncorhynchus mykiss) Possess a Large Core Intestinal Microbiota That Is Resistant to Variation in Diet and Rearing Density

High or low dietary carbohydrate:protein ratios during first-feeding affect glucose metabolism and intestinal microbiota in juvenile rainbow trout

The Gut Microbiota of Fish

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