Characterization of Ralstonia solanacearum Causing Bacterial Wilt Disease of Tomato in Coimbatore District of Tamil Nadu, India

Balamurugan, A.; Muthamilan, M.; Kamalakannan, A.; Shanthi, A.; Arumugam, T.

doi:10.20546/ijcmas.2020.902.345

Cited by 8 publications

(3 citation statements)

References 12 publications

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“…Most species of Ralstonia are related to plant pathogens. For instance, Ralstonia solanacearum can cause bacterial wilt disease (Balamurugan et al, 2020 ). An increase in the abundance of Ralstonia may have been the reason for the increase in the abundance of plant–pathogen functional groups (Figure 6b ).…”

Section: Discussionmentioning

confidence: 99%

Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation

Liu

Zhu

et al. 2021

MicrobiologyOpen

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Cigar tobacco leaves (CTLs) contain abundant bacteria and fungi that are vital to leaf quality during fermentation. In this study, artificial fermentation was used for the fermentation of CTLs since it was more controllable and efficient than natural aging. The bacterial and fungal community structure and composition in unfermented and fermented CTLs were determined to understand the effects of microbes on the characteristics of CTLs during artificial fermentation. The relationship between the chemical contents and alterations in the microbial composition was evaluated, and the functions of bacteria and fungi in fermented CTLs were predicted to determine the possible metabolic pathways. After artificial fermentation, the bacterial and fungal community structure significantly changed in CTLs. The total nitrate and nicotine contents were most readily affected by the bacterial and fungal communities, respectively. FAPROTAX software predictions of the bacterial community revealed increases in functions related to compound transformation after fermentation. FUNGuild predictions of the fungal community revealed an increase in the content of saprotrophic fungi after fermentation. These data provide information regarding the artificial fermentation mechanism of CTLs and will inform safety and quality improvements.

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Section: Discussionmentioning

confidence: 99%

Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation

Liu

Zhu

et al. 2021

MicrobiologyOpen

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“…This disease has become a major constraint for tomato growers worldwide with yield losses estimated to range from 35% to 90% in India alone (Gupta & Kamalachandran, 2018). In fact, R. solanacearum is ranked as the second‐most important bacterial plant pathogen globally in terms of scientific and economic significance (Balamurugan et al., 2020). It causes rapid wilting in more than 310 species of plants belonging to 42 plant families (Paudel et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antagonistic activity of DAPG‐producing fluorescent pseudomonads from rhizospheres of Solanaceous crops for biocontrol of bacterial wilt

Suansia,

Senapati,

Samantaray

et al. 2024

Journal of Phytopathology

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Pseudomonads produce a wide array of antimicrobial compounds including 2,4‐diacetylphloroglucinol (DAPG), which possess a broad‐spectrum antagonistic effect against phytopathogens. Rhizospheric soil samples were collected from the Solanaceous crops for isolation of fluorescent pseudomonads (FPs). Out of the 32 fluorescent bacterial isolates, 21 showed antagonistic activity against Ralstonia solanacearum (NAIMCC‐B‐01630) with the highest zone of inhibition at 38.33 mm. Among them, 11 bacterial isolates depicted the production of DAPG in Kings' B medium. DAPG production was detected by TLC and quantified through HPLC analysis. Moreover, the bacterial isolate OD13 exhibited higher DAPG production. Further, the presence of the DAPG‐encoding phlD gene within the size of 629 bp and 725 bp was also validated through amplification. These bacterial isolates belonged to the genera Pseudomonas as confirmed by morpho‐physiological characterization using the VITEK 2 system. The higher DAPG‐producing bacterial isolate was identified as Pseudomonas aeruginosa by polyphasic approach with GenBank accession number OQ781265. Thus, the DAPG‐producing rhizospheric Pseudomonas strains can be used as biocontrol agents for bacterial wilt. However, field study is highly essential before its successful application.

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“…The bacteria enter the plant roots from the soil and multiply in and gradually block the vascular system causing wilting within a few days of infection often associated with yellowing of the youngest leaves. Under favorable conditions, the infected plant suddenly wilts and dies [3]. There is no efficient method to control bacterial wilt in tomato due to the broad host range, complex diversity of the pathogen, long-term survival in the soil, and latent infection in the host [4].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Biocontrol Agents Against Bacterial Wilt in Tomato Using Seedling Screening

Maxwell¹,

Chang²,

Chen³

et al. 2022

Proceedings of the International Symposium Southeast Asia Vegetable 2021 (SEAVEG 2021)

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Tomato is among the most cultivated vegetable crops worldwide, and bacterial wilt (BW) caused by the Ralstonia solanacearum species complex (RSSC) is the most devastating disease affecting tomato, impacting food and nutrition security in many areas. Pesticides used for controlling plant diseases are hazardous to producers, consumers, and the environment, whereas biological control is potentially a sustainable and environmentally safe alternative for disease management. To identify efficient biocontrol agents (BCAs), twenty-five potential BCA isolates were screened for control efficacy to BW on ten-day-old tomato seedlings of highly susceptible (L390) and moderately resistant (L180) cultivars previously inoculated with R. solanacearum strain PSS4 (=Asian origin, Race 1, Phylotype I; Biovar 3). After ten days incubation at 28 °C in the growth chamber, wilting (W%) and biocontrol efficacy (BE%) percent were evaluated. Of the 25 BCAs tested, four significantly reduced W%, with BE% ranging from 50% to 80% for both varieties. The four BCA isolates were identified as Talaromyces sp., Trichoderma sp., Bacillus sp., and Variovorax sp. The seedling method allows the rapid and cost-effective in vivo screening of many potential BCAs to reliably identify those with higher bacterial wilt control efficacy for further testing.

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Characterization of Ralstonia solanacearum Causing Bacterial Wilt Disease of Tomato in Coimbatore District of Tamil Nadu, India

Cited by 8 publications

References 12 publications

Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation

Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation

Evaluation of antagonistic activity of DAPG‐producing fluorescent pseudomonads from rhizospheres of Solanaceous crops for biocontrol of bacterial wilt

Evaluation of Biocontrol Agents Against Bacterial Wilt in Tomato Using Seedling Screening

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