Characterization of rat and human Kupffer cells after cryopreservation

Walbrun, Peter; Hellerbrand, Claus; Weiss, Thomas S.; Netter, Susanne; Gaebele, Erwin; Wiest, Reiner; Schöelmerich, Juergen; Froh, Matthias

doi:10.1016/j.cryobiol.2006.12.005

Cited by 12 publications

(10 citation statements)

References 24 publications

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“…However, the slow-cooling rate allows the cells to dehydrate by maintaining equilibrium with the partially frozen extra-cellular solution [37]. Although with only a few exceptions, a cooling rate of 1 C/min is recommended [38]. However, a regimen found effective for one cell type may not be effective for others.…”

Section: Discussionmentioning

confidence: 98%

Cryopreservation based on freezing protocols for the long-term storage of microencapsulated myoblasts

Murua¹,

Orive²,

Hernández³

et al. 2009

Biomaterials

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Section: Discussionmentioning

confidence: 98%

Cryopreservation based on freezing protocols for the long-term storage of microencapsulated myoblasts

Murua¹,

Orive²,

Hernández³

et al. 2009

Biomaterials

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“…KCs, the resident hepatic macrophages, are considered HCC-specific TAMs. KCs make up less than 5 % of the volume of the liver; however, they represent about 80 % of the total fixed macrophage population in the human body [11]. Owing to their wide range of biological functions in immune regulation, inflammation, and oncogenesis, KCs are involved in the pathogenesis of several liver diseases.…”

Section: Introductionmentioning

confidence: 99%

Deletion of interleukin-6 in monocytes/macrophages suppresses the initiation of hepatocellular carcinoma in mice

Kong

Zhou

et al. 2016

J Exp Clin Cancer Res

127

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BackgroundHepatocellular carcinoma (HCC) is associated with inflammation, and roughly 30 % of the global population shows serological evidence of current or past infection with hepatitis B or hepatitis C virus. Resident hepatic macrophages, known as Kupffer cells (KCs), are considered as the specific tumor-associated macrophages (TAMs) of HCC, and can produce various cytokines—most importantly interleukin (IL)-6—to promote tumorigenesis of HCC. However, the roles of KCs and IL-6 in carcinogenesis in the liver are still unclear.MethodsWe analyzed leukocyte-related peripheral blood data of 192 patients and constructed a mouse model in which the bone marrow was cleared out by irradiation and reconstructed using bone marrow donated from IL-6-deficient mice to further elucidate the hepatic pathological changes in response to toxic challenge and oncogenic gene mutation.ResultsPeripheral monocyte counts and serum IL-6 levels were significantly higher in patients with HCC than in those without HCC. In addition, there was a significant difference in the levels of IL-6 among individuals with different histopathological grades. In mice with selective IL-6 ablation in monocytes/KCs, we observed decreased toxic liver injury, inflammatory infiltration, and systemic inflammation. In Mdr2-deficient mice, which spontaneously developed HCC, the loss of IL-6 in monocytes/KCs resulted in inhibition of IL-6/signal transducer and activator of transcription 3 signaling, decreased serum IL-6 levels, and delayed tumorigenesis.ConclusionsOur findings demonstrate that increased TAM-derived IL-6 had an amplifying effect on the inflammation response, thereby promoting the occurrence and development of HCC.

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“…The isolation of NPC is a younger discipline and cryopreservation is still under development. Successful cryopreservation of rat and human Kupffer cells with high post-thawing cell survival and no alterations in physiological characteristics were introduced by Walbrun et al, 17 using a standard cryopreservation technique based on RPMI medium with FCS and DMSO frozen down to -80 C using a cooling gradient. The cryopreservation of hepatic stellate cells requires more elaborated preservation methods due to the sensitivity of intracellular lipid droplets to freezing.…”

Section: Cryopreservation Of Primary Human Liver Cellsmentioning

confidence: 99%

Cell sources forin vitrohuman liver cell culture models

Zeilinger

Freyer

Damm

et al. 2016

Exp Biol Med (Maywood)

186

160

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In vitro liver cell culture models are gaining increasing importance in pharmacological and toxicological research. The source of cells used is critical for the relevance and the predictive value of such models. Primary human hepatocytes (PHH) are currently considered to be the gold standard for hepatic in vitro culture models, since they directly reflect the specific metabolism and functionality of the human liver; however, the scarcity and difficult logistics of PHH have driven researchers to explore alternative cell sources, including liver cell lines and pluripotent stem cells. Liver cell lines generated from hepatomas or by genetic manipulation are widely used due to their good availability, but they are generally altered in certain metabolic functions. For the past few years, adult and pluripotent stem cells have been attracting increasing attention, due their ability to proliferate and to differentiate into hepatocyte-like cells in vitro. However, controlling the differentiation of these cells is still a challenge. This review gives an overview of the major human cell sources under investigation for in vitro liver cell culture models, including primary human liver cells, liver cell lines, and stem cells. The promises and challenges of different cell types are discussed with a focus on the complex 2D and 3D culture approaches under investigation for improving liver cell functionality in vitro. Finally, the specific application options of individual cell sources in pharmacological research or disease modeling are described.

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Characterization of rat and human Kupffer cells after cryopreservation

Cited by 12 publications

References 24 publications

Cryopreservation based on freezing protocols for the long-term storage of microencapsulated myoblasts

Cryopreservation based on freezing protocols for the long-term storage of microencapsulated myoblasts

Deletion of interleukin-6 in monocytes/macrophages suppresses the initiation of hepatocellular carcinoma in mice

Cell sources forin vitrohuman liver cell culture models

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